CBS 89, 10-11 (2002). HPTLC on silica gel with 1) ethyl acetate - chloroform 1:1 over 30 mm followed by drying at room temperature for 15 min and 2) ethyl acetate - chloroform 9:1 over 50 mm in horizontal development chamber. Detection by dipping in anisaldehyde - sulfuric acid reagent followed by heating at 60 °C for 5 min. Quantitative determination by absorbance measurement at 440 nm. Precision (n=6 at 100 mg/kg feed) is found to be 2.7 %.

CBS 81, 14-15 (1998). HPTLC-AMD of extracts on silica gel with 16-step gradient based on methanol containing 0.1 % acetic acid via dichloromethane to n-hexane. Quantification by absorbance measurement at 255, 260, 290 and 310 nm (multi wavelength scan). Precision is determined to be 3%.

CBS 83, 14-15 (1999) HPTLC of a mixture of the reaction product and its educts on cyano phase with cyclohexane - ethyl acetate - acetic acid 400:100:1 over 40 mm. Quantitative determination by absorbance measurement at 233 nm.

J. Liq. Chrom. Rel. Technol. 27, 2039-2045 (2004). HPTLC of cholesteryl oleate, methyl oleate, triolein, oleic acid, and cholesterol on pre-washed silica gel with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 in a twin-trough chamber with saturation. Detection with phosphomolybdic acid reagent. Quantitative determination at 610 nm.

J. AOAC Int. 86, 935-940 (2003). TLC of indapamide (4-chloro-N-(2-methylindolin-1-yl)-3-sulfamoylbenzamide), its degradation product, and related substance 2-methylnitrosoindoline on silica gel using toluene - ethyl acetate - glacial acetic acid 69:30:1 after pre-saturation for 1 h. Detection under UV light at 254 nm. Quantitative determination at 424 nm. Limit of detection 0.11 µg/spot. The method was validated according to the guidelines of the USP.

J. Liq. Chrom. Rel. Technol. 27, 2047-2056 (2004). TLC of bromhexin on silica gel with n-butanol - glacial acetic acid - water 260:77:75 in a twin-trough chamber after at least 3 h of saturation. Quantitative determination at 325 nm by absorbance measurement. The method is selective, precise, and accurate and can be used for routine analysis of pharmaceutical preparations in pharmaceutical industry quality control laboratories.

J. Planar Chromatogr. 17, 261-263 (2004). HPTLC of aglycones and glycosides (flavone, hesperetin, naringenin, apigenin, kaempferol, quercetin, myricetin, tiliroside, apigenin 7-glucoside, myricitrin, kaempferol 3,7-dirhamnoside, hyperoside, hesperidin, rhoifolin, rutin, naringin) on silica gel by using mixtures of dichloromethane and ethyl acetate for aglycones and mixtures of ethyl acetate and formic acid for glycosides. The plates were conditioned for 15 min and developed face down in a horizontal chamber. To separate mixtures of flavonoids two-step gradient devlopment was used. In the first step (for gycosides) the plates were developed to a distance of 65 mm with ethyl acetate - formic acid - water 170:30:1. In the second step (for aglycones ) the plates were developed to a distance of 95 mm with dichloromethane - ethyl acetate - formic acid 170:30:1. Quantitation by densitometry at 254 nm.

J. Planar Chromatogr. 17, 479-482 (2004) HPTLC of beta-boswellic acid (BA), acetyl-beta-BA, keto-BA, and acetyl-keto-BA and ethanolic extracts of olibanum resin on silica gel with toluene - ethyl acetate - formic acid - heptane 80:20:3:10 in a twin trough chamber without chamber saturation. Quantitative determination by reflectance measurement at 245 and 285 nm. Also two dimensional development with the same mobile phase in the second direction.