Biochem. Biophys. Res. Commun. 399, 716-720 (2010). HPTLC of globotriaosylceramide (Gb3) in mouse tissues on silica gel with chloroform - methanol - water 65:25:4. Detection by HPTLC-immunostaining with an anti-Gb3 monoclonal antibody. Quantification by densitometry with a luminescent image analyzer. The limit of detection of Gb3 was 50 ng/zone.

Analytical Chemistry - An Indian Journal 8(1), 77-81 (2009). An HPTLC method is reported for estimation of lupeol and beta-sitosterol in the whole plant powder of Asteracantha longifolia (Acanthaceae). The proposed method was also employed for estimation of beta-sitosterol in herbal formulation containing Asteracantha longifolia. Methanolic extracts of the sample were used for chromatography. HPTLC on silica gel with toluene - ethyl acetate - methanol 75:15:7. Derivatization with Liebermann-Burchard reagent. Densitometric quantification at 366 nm. The concentration of lupeol and beta-sitosterol was 0.162 mg/g and 0.045 mg/g, respectively in the whole plant, while the concentration of beta-sitosterol was 0.039-0.048 mg/g in the formulation. The method was suitable for the quality control of the herbal formulation.

Indian Drugs 47(5), 42-45 (2010). HPTLC of lamivudine and zidovudine on silica gel with acetone - toluene - methanol 2:1:2. The hRf of lamivudine was 34 and of zidovudine 74. Densitometric quantification at 267 nm for zidovudine and 272 nm for lamivudine. The method was linear in the range of 10-210 ng/band for lamivudine and 30-420 ng/band for zidovudine. The recovery was between 99.5-100.4 % for the compounds. The method is suitable for estimation of compounds in combined formulations.

J. Pharma. Sci. & Res. 2(8), 477-483 (2010). TLC on silica gel with ethyl acetate - glacial acetic acid - methanol - water 10:5:3:2. The hRf value was 51 and 74 for amoxicillin and bromhexine, respectively. Densitometric evaluation at 260 nm (bromhexine) and at 320 nm (amoxicillin). The method was linear in the range of 200-1000 ng/band and 10-30 µg/band for bromhexine and amoxicillin, respectively. The recovery was between 98.1-101.5 %.

Research J. Pharm. and Tech. 3(2), 559-561 (2010). TLC on silica gel with toluene - acetonitrile - formic acid 50:50:3 with chamber saturation for 30 min. The hRf value was 27 for perindopril and 55 for losartan. Densitometric evaluation at 212 nm. The method was linear in the range of 5-30 µg/band and 1-10 µg/band for losartan and perindopril respectively. The average recovery was 98.2-99.9 %.

Journal of Pharmaceutical Research 9(2), 60-62 (2010). TLC on silica gel with chloroform - ethyl acetate - methanol - ammonia 20:20:10:1 with chamber saturation for 30 min. Densitometric evaluation at 232 nm. The method was linear in the range of 100-800 ng/band for amlodipine, 400-2400 ng/band for hydrochlorothiazide and 1000-8000 ng/band for amlodipine. The average recovery was 99.1-100.4 % for all three compounds. The method was suitable for stability studies (stress conditions: acid, base, thermal, UV).

62nd Indian Pharmaceutical Congress Abstract No. F-259 (2010). Health drinks usually contain several phytopharmaceuticals with immunomodulatory and antioxidant activities. TLC of gallic acid on silica gel with toluene – ethyl acetate – methanol – formic acid 15:15:1:4. The gallic acid content was established and the identity of the gallic acid zone in sample and standard was confirmed by UV spectra comparison.

62nd Indian Pharmaceutical Congress Abstract No. F-384 (2010). TLC of isotretinoin on silica gel with toluene – ethyl acetate 4:1. The hRf value was 54. Quantitative determination by absorbance measurement at 345 nm. The method was linear in the range of 20-100 ng/band. The sample was analysed by RP-HPLC and the result was comparable with the TLC method.