J. Chinese Trad. Patent Med. (Zhongchengyao) 20 (5), 11-13 (1998). TLC on silica gel with 1) chloroform - ethyl acetate - methanol - formic acid 400:50:100:2, 2) methanol - acetone - chloroform - NH3 6:8:26:1, 3) chloroform - methanol 5:1, 4) cyclohexane - ethyl acetate 1:1. Detection 1) by spraying with 5% vanillin-sulfuric acid reagent and heating at 105°C for 5 min, 2) by spraying with 0.5% ninhydrin in ethanol and heating at 105°C for 10 min. Identification by finger print technique. Quantitation of paeoniflorin by spectrophotometry at 230 nm after elution. Recovery 97.3 ± 1.1% (n=3).

CBS 90, 6-7 (2003). HPTLC phospholipids and glycolipids from rape seed on lichrospher silica gel with chloroform - methanol - acetone - water 18:15:2:1 for phospholipid separation and with acetone - chloroform - water 30:15:2 for glycolipid separation, developing distance 70 mm each. Detection by dipping in molybdatophosphoric acid reagent (5 % in ethanol) followed by heating at 120 °C for 15 min. Quantitative determination by absorbance measurement at 720 nm.

Analysis of choline esterase inhibitors. CBS 83, 4-5 (1999) HPTLC-AMD of choline esterase inhibitors on silica gel with a 15-step gradient from methanol via dichloromethane to hexane. Detection by spraying with butyryl choline esterase solution followed by incubation at 37 °C for 30 min, and spraying with fast blue salt mixed with alpha-naphthyl acetate followed by incubation at 37 °C for 5 min. Quantitative determination by absorbance measurement at 254 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 26 (2), 172-173 (2004). TLC on silica gel with 1) benzene - acetone - ethyl acetate - conc. ammonia 10:15:20:2. Detection by spraying with 5 % potassium iodobismuthate solution. Identification by comparison with the standard. Quantification by densitometry at 524 nm. The procedure is validated by investigation of its repeatability, reproducibility, and recovery. Discussion of the merits of the procedure compared to that in China Pharmacopeia (2000 ed).

CBS 83, 12-13 (1999) HPTLC of levamisol in pig tissue on silica gel with chloroform over a developing distance of 70 mm, then with chloroform - methanol - formic acid 400:50:1 over 50 mm. Quantitative determination by absorbance measurement at 220 nm. Limits of detection are 0.6 - 1.5 µg/kg.

CBS 93, 10-12 (2004). HPTLC of progesterone on silica gel prewashed by development in AMD2 first with chloroform - methanol 1:1 and then with the mobile phase, followed by drying at 80 °C for 15 min. Development in AMD2 with toluene - 2-propanol 10:1 without preconditioning over 60 mm. Quantitative determination by absorbance measurement at 252 nm followed by spectra recording from 200 to 360 nm. The linear working range is 25.7-154.5 ng/zone. Repeatability (standard deviation calculated from the amounts of seven simulated progesterone samples determined on the same plate at three concentration levels in the lower, middle and upper range) is 0.26-1.29 %. Recovery is 99.88-100.97 %. Reproducibility was performed with recycled HPTLC plates.

J. Liq. Chrom. Rel. Technol. 27, 2057-2070 (2004). HPTLC of pioglitazone hydrochloride ((±)-5-[p-[2-(ethyl-2-pridinyl)ethoxy]-benzyl]-2,4-thiazolidinedione hydrochloride), rosiglitazone maleate ((±)-5-[4-[2-[N-methyl-N-(2--pyridinyl)amino]-ethoxy]benzyl]-2,4-thiazolidinedione maleate), and repaglinide S(+)-2-ethoxy-4-[N-[1-(2-piperidinophenyl)-3-methyl-1-butyl]-aminocarbonylmethyl]benzoic acid on cyano-phase with 1,4-dioxane - buffer pH 2.8/4.4/6.4/7.9 1:4; 2:3; 1:1; 3:2; 2:1 in horizontal chambers without saturation. Quantitative determination of pioglitazone by reflectance/transmittance measurement at 266 nm.

J. Planar Chromatogr. 17, 75-78 (2004). HPTLC of montelukast sodium (1-[[[(1R)-1-[3-[(1E)-2-(7-chloro-2-quinolinyl)ethenyl]phenyl]-3-[2-(1-hydroxy-1-methylethyk)phenyl]-propyl]thio]methyl] cyclopropane acetic acid monosodium salt) and nimesulide (N-(4-nitro-2-phenoxyphenyl)methane sulfonamide or 4-nitro-2-phenoxymethanesulfonanilide) as internal standard on pre-washed silica gel with toluene - ethyl acetate - glacial acetic acid 60:34:1 in a twin-trough chamber. Quantitative determination at 344 nm.