Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (4), 343-345 (2004). TLC of ephedrine chloride on silica gel with chloroform - methanol - ammonia 200:35:6. Detection by spraying with 0.5 % ninhydrin in ethanol followed by heating at 105 ºC for a few minutes. Quantitative determination at 510 nm. Validation of the method by investigation of linear range of the calibration curve (0.42 - 2.10 µg/spot, R = 0.999), precision (RSD = 2.1 %, n=6 within plate and 2.7 %, n = 5 plate-to-plate), reproducibility (RSD = 2.3 %, n = 5), and recovery (98.1 %, n = 6). The results obtained by using the method are given towards some real-life samples.

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (1), 16-19 (2004). TLC of neutral sugars, rhamnose, xylose, and glucose, extracted from Ulva pertusa alga, on silica gel with n-butanol - ethyl acetate - isopropanol - acetic acid - water - pyridine 7:20:12:7:6:6. Detection by spraying with a solution of aniline hydrogen phthalate (0.93 g) – phthalic acid (1.66 g) – water saturated n-butanol (100 mL), followed by heating at 110 ºC for 10 min. Identification and quantification by comparison with the standards. Application of an orthogonal test to investigate the effects of four factors (volume of water, temperature, extraction time, and pH) on the yield of the compounds, and determination of the optimum extraction conditions.

J. Planar Chromatogr. 17, 181-185 (2004). HPTLC of an inclusion complex of coenzyme Q10 with beta-cyclodextrin on silica gel by one-dimensional, two-dimensional, and multi-dimensional separation with 1) dioxane - water 1:1 and 2) chloroform - methanol 11:9. Detection by spraying with 5 % phosphomolybdic acid in ethanol and drying at 110 °C, followed by spraying with 50 % sulfuric acid and heating at 120 °C for 5 min.

IPC 56th 2004, Abstract No. G-20. Simultaneous HPTLC determination of loratadine and ambroxol in combined dosage form on silica gel with n-hexane - dichloromethane - triethanolamine 11:8:1.The Rf value of loratadine and ambroxal was found to be 0.40 and 0.16 respectively. Quantitative evaluation by scanning at 254 nm.The method was linear in the range of 0.2 -1 mg/spot for loratadine and 1.2 - 6 mg/spot for ambroxol with recovery of 98.2 - 98.5 %. The method was validated for accuracy, precision, linearity, specificity, LOD, and LOQ.

56th IPC 2004, Abstract No. GP-36. HPTLC for the standardization of gallic acid in alcoholic extracts of Aphanamixis polystachya (Meliceae) on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:4:1. Rf value of gallic acid was 0.45, linearity was 15 - 75 mg/mL. Formulations were found to contain 9.56 % of gallic acid. Gallic acid is the main phenolic compound and can be used for standardization of the crude drug.

- Part 1. Fundamentals of systematic quantitative errors. J. Planar Chromatogr. 18, 51-56 (2005). First part of a series covering systematic errors arising in separation systems, evaluation and calibration errors, nonlinear separation and quantitation techniques, the sf4-procedure for finding total systematic errors, systematic errors caused by regulations, and conclusions and proposals for quantitative planar liquid chromatography (PLC). 1) Nonsystematic errors: 1.1 Finding systematic errors hidden in nonsystematic errors, 1.2 Are random errors unstable? 1.3 Errors caused by (false) statements. 2) The main sources of systematic errors in quantitative PLC: 2.1 Chromatography, 2.2 Physics (baseline - baseplane; practical example). 2.3 Mathematics (detectability limit of the systematic error and the standard certainty STC). 3) Sampling chromatography.

CBS 95, 2-4 (2005). TLC of seven biogenic amines (extracted with trichloroacetic acid from fish, followed by dansylation with dansyl chloride) on silica gel (prewashed with developing solvent) with benzene - chloroform - triethylamine 10:6:7 or 10:6:2 in horizontal developing chamber over 90 mm. Quantitative determination by fluorescence measurement at 365 nm/> 400 nm. Calibration using peak area, LOD 7.5 mg/kg, LOQ 56 mg/kg.

J. AOAC Int. 87, 827-833 (2004). TLC of trimetazidine dihydrochloride and degradation products (e. g. piperazine and 2,3,4-trimethoxymethyl benzene) on silica gel with methanol - ammonia 200:3. Detection under UV light at 254 nm and densitometry at 215 nm. The method was applicable over a concentration range of 2.00-9.00 µg/spot with a mean percentage accuracy of 99.86 +/- 0.92.