J. AOAC Int. 93, 820-824 (2010). HPTLC of mexiletine hydrochloride on RP-18 with tetrahydrofuran - citrate buffer (pH 4.45) 3:7 and on amino phase with chloroform - tetrahydrofuran - hexane - ethylamine 30:20:50:1. Quantitative determination by absorbance measurement at 217 nm. Linearity was between 0.5 and 8.0 µg/spot. The accuracy was 99.6 % for the amino phase and 99.5 % for the RP-18 phase. The %RSD of intra-day and inter-day precision was 1.2 and 2.7 %, respectively; for both layers LOD and LOQ were 100 and 300 ng/zone, respectively.

J. Planar Chromatogr. 24, 53-56 (2011). HPTLC of the pesticides temephos and fenitrothion on silica gel, prewashed with methanol, with acetone - hexane 3:7 in an unsaturated twin-trough chamber. Quantitative determination by densitometry in absorbance mode at 290 nm. The hRf values were 55 and 69. LOD was 20 ng for temephos and 10 ng for fenitrothion. Recovery was 80-107 % with relative standard deviations of 4.4-20.2 %.

J. Planar Chromatogr. 24, 40-43 (2011). TLC of fluoxetine and citalopram on RP-18 with methanol - 0.05 M phosphate buffer (pH 5) - triethylamine 68:27:5 after chamber saturation for 15 min. Detection under UV light at 254 nm. Quantitative determination by densitometry in absorbance mode at 230 nm. Linearity was between 500 and 5000 ng/zone. Recovery was in the range of 100.8-101.1 % for fluoxetine and 99.7-100.9 % for citalopram. Corresponding %RSD values were less than 1.6 % for fluoxetine and 0.8 % for citalopram.

J. Liq. Chromatogr. Relat. Technol. 34, 1664-1675 (2011). HPTLC of pramipexole in pharmaceutical formulations on silica gel with ethyl acetate - toluene - methanol 16:3:1 + 1 drop ammonia. Quantitative determination by absorbance measurement at 263 nm. The hRf value of pramipexole was 32. Precision was below 2 %. Linearity was 200-2000 ng/zone. LOD and LOQ were found to be 30 and 200 ng/zone. The intermediate/inter-day/intra-day precision (n = 6) was 0.4 %. Recovery (by standard addition) was in the range of 98.5-99.1 %.

Anal. Chim. Acta 716, 54-60 (2012). Demonstration of the separation and detection capability of micro-TLC technique involving simple one step liquid extraction protocols of complex materials without multi-step sample pre-purification. Isolation of the target components (cyanobacteria pigments, lipids and fullerenes) from complex matrices including spirulina dried cells, birds’ feathers, fatty oils, and soot samples derived from biomass fuel and fossils-fired home heating systems. Completion of an isocratic separation protocol involving less than 1 mL of one component or binary mixture mobile phases within times of 5–8 min in each case, e.g. 1) micro-TLC of dyes and low-molecular mass compounds of cyanobacteria cells (S. platensis) extracted from pharmaceutical formulations on RP-18W phase with acetone – n-hexane 3:7 detection under daylight and densitometric evaluation; 2) separation of the main lipids fraction derived from bird feathers and oil samples (rapeseed, grapeseed, sunflower and olive oil) on RP-18 phase with dichloromethane – methanol 3:17, detection by spraying with 10 % phosphomolybdic acid in methanol and heating at 80 °C for 20 min; 3) screening of soot residues in dust samples derived from biomass fuel and fossils home heating systems for the presence of C60/C70 fullerenes on RP-18W phase with n-hexane, detection by densitometry at visible light, or at UV 254 nm or UV 366 nm.

CBS 109, 2-4 (2012). Extraction of arabica coffee with 2 to 50 % robusta coffee by accelerated solvent extraction with tBME. A part was saponified with10 % ethanolic KOH solution for 2 h. HPTLC of coffee extracts and standards 16-O-methylcafestol (16-OMC) and 16-OMC esters on silica gel with toluene - ethyl acetate - acetic acid 93:7:1 for 16-OMC esters and with tBME - chloroform 1:1 for 16-OMC. Detection by spraying with vanillin sulfuric acid (1 g in 250 mL ethanol and 2 mL of sulfuric acid, prepared freshly) and heating for 1 min at 80 °C. Quantitative absorption measurement at 530 nm. The LOD was 163 ng/band. After saponification the LOD of free 16-OMC was 43 ng/band. Thus 2 % robusta can be detected in a coffee blend.

J. AOAC Int. 95, 1614-1617 (2012). HPTLC of (E)-4-(3,4-dimethoxyphenyl)butadiene (DMPBD) in the rhizome of Zingiber cassumunar on silica gel with hexane - dichloromethane 3:2. Quantitative determination by absorbance measurement at 289 nm. The hRf value of DMPBD was 30. Linearity was between 130 and 703 ng/zone. LOD and LOQ were 40 and 10 ng/zone, respectively. The interday and intra-day precisions were 0.9 and 0.3 % (n=3). Average recovery (by standard addition) was 103.1 %.

J. Planar Chromatogr. 27, 204-209 (2014). HPTLC of naringin in the leaves and stems of Rumex vesicarius on silica gel with ethyl acetate - glacial acetic acid - methanol - water 30:10:5:1. Quantitative determination by absorbance measurement at 275 nm. The hRF value for naringin was 46. Linearity was in the range of 100-1000 ng/zone. The intermediate/interday/intra-day precisions were below 2 % (n=6). The LOD and LOQ were 37 and 111 ng/zone, respectively. The average recovery was 99.5 %.