J. Chinese Herb. Med. (Zhongcaoyao) 28, 470-471 (1997). TLC on silica gel with cyclohexane - ethyl acetate - diethyl amine 6:4:1. Detection by spraying with potassium iodobismuthate reagent. Quantitation by densitometry at 505 nm. Precision within plate 0.52%, 0.46% resp. (n=4), precision plate-to-plate 1.23%, 1.46%, resp. (n=5). Recoveries 98.2 ± 1.6% (n=5), 98.6 ± 1.7 (n=5), resp.

CBS 82, 12-15 (1999). HPTLC of ergometrine, ergotamine, and ergocryptine on silica gel with dichloromethane - methanol 9:1 with chamber saturation. Quantification by fluorescence measurement at 245/>310 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 26 (1), 29-32 (2004). TLC on silica gel with 1) chloroform - ethyl acetate - methanol - water 15:40:22:10, 2) chloroform - methanol - water 13:7:2. Detection 1) by spraying with 10 % H2SO4 in ethanol and heating at 105 ºC, 2) under UV light. Identification by fingerprint technique. Quantification of chlorogenic acid by HPLC.

CBS 86, 10-12 (2001) HPTLC of valerian root extracts on silica gel (prewashed with methanol) with chloroform - ethyl acetate - propanol 15:10:1. Detection by dipping for 1 s in 15 % sulfuric acid in methanol followed by heating at 120 °C for 10 min and cooling to room temperature. Evaluation with video technology (VideoStore/VideoScan).

CBS 93, 14-15 (2004). HPTLC of heterocyclic aromatic amines on silica gel WRF (prewashed with methanol by chromatography followed by drying at 120 °C for 30 min) with a 6 step gradient with diethyl ether, methanol and chloroform, with alkaline conditioning via gas phase over max. 60 mm. Quantitative determination by multi-wavelength scan in absorbance mode at 252, 262, 316 nm and fluorescence measurement at 366/>400 nm. Polynomial evaluation via peak area and height. Limit of quantitation is between 1-45 ng absolute on the plate. Online coupling with MS by use of a new extractor developed by Luftmann.

Indian J. Pharm. Sci. 66 (3), 306-308 (2004). HPTLC on silica gel with n-butanol - methanol - 6 M ammonia 5:1:2. Gatifloxacin showed Rf values of 0.47 ± 0.03. Quantitative determination by absorbance measurement at 292 nm. The method was validated in terms of linearity (400-1200 ng/spot), precision (intra-day variation 1.3 to 3.2 %, inter-day variation 3.9 to 5.0 %), accuracy (93.3 to 99.4 %), and specificity. The limit of detection and limit of quantification for gatifloxacin were found to be 10 ng/spot and 50 ng/spot respectively. The method is simple, sensitive, precise, and can be used for the routine quality control testing of marketed tablet formulations (400 mg).

Indian Drugs 41 (6), 354-357 (2004). HPTLC on silica gel with toluene - methanol - acetone - 10 % ammonia 80:40:20:1. Quantitative determination by absorbance measurement at 254 nm. The Rf value of pioglitazone hydrochloride was found to lie between 0.49-0.55. The linear dynamic response was found to be 2.0-4.0 µg/spot for pioglitazone hydrochloride. The results of the analysis have been validated statistically and by recovery studies. A simple, fast, specific and precise HPTLC method has been developed for the estimation of pioglitazone hydrochloride in its tablet dosage form.

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (3), 248-249 (2004). TLC of astragaloside on silica gel with the lower phase of chloroform - methanol - water 13:6:2. Detection by spraying with 10 % H2SO4 in ethanol and heating at 105 ºC. Identification by comparison with the standard. Quantification by densitometry at 530 nm. Validation of the method by investigation of precision (RSD=2.2 % n=5 within plate and 3.0 % n=5 plate-to-plate), linearity range (0.45-2.28 µg/spot r=0.998), repeatability, reproducibility, and recovery (98.1 %, n=5, RSD=3.4 %).