Rec. Nat. Prod. 3/4, 178-186 (2009). An HPTLC method has been reported for quantitative estimation of alpha-mangostin in fruit pericarp of Garcinia mangostana (Hypericaceae). HPTLC on silica gel with chloroform - methanol 9:1 in a saturated chamber (10 min). The plate was derivatized with anisaldehyde sulfuric acid reagent. Densitometric quantification at 382 nm. Alpha-mangostin was detected as a compact band with hRf value of 46. The method was linear in the range of 1-5 µg/band. The maximum yield of alpha-mangostin was obtained from the plant by hot extraction with methanol.

B. Fried, R. Toledo (Eds): Biomphalaria snails and larval trematodes. Springer 20119. Chapter 4. This book chapter reviews TLC, HPTLC and other methods for the analysis of larval trematodes from Biomphalaria snails. The analysis of different classes of organic compounds is described: neutral and polar lipids, glycolipids, lutein, beta-carotene, sugars and amino acids. Various TLC and HPTLC phases and developing solvents were used.

62nd Indian Pharmaceutical Congress Abstract No. F-252 (2010). TLC of alizarin and betulinic acid on silica gel with toluene – ethyl acetate – formic acid 18:3:1. The hRf values were 53 and 58 for betulinic acid and alizarin, respectively. Quantitative determination by absorbance measurement at 287 nm. The method was linear in the range of 60-160 ng/band for alizarin and 300-800 ng/band for betulinic acid. The average recovery was in the range of 99.4-99.6 % for both compounds.

62nd Indian Pharmaceutical Congress Abstract No. F-244 (2010). TLC of fluvoxamine maleate on silica gel with benzene – methanol 5:4. The hRf value was 52. Quantitative determination by absorbance measurement at 256 nm. The linearity was in the range of 500-3000 ng/band with r2=0.998. The drug was subjected to acidic, alkaline and oxidative, dry heat, UV and photolytic stress. Since the method could effectively separate the drug from its degradation products, it can be used for stability studies.

62nd Indian Pharmaceutical Congress Abstract No. F-263 (2010). TLC of desloratadine on silica gel with methanol – chloroform – toluene – ammonia 50:50:10:3. Quantitative determination by absorbance measurement at 254 nm. The hRf value was 61. The linearity was in the range of 150-750 ng/zone with r2 = 0.9997. The limit of detection was 21 ng/zone, whereas the limit of quantitation was 65 ng/zone.

Acta Chromatographica 20(4), 673-683 (2008). Presentation of a quantitative method for the analysis of lycopene in nutritional supplements consumed to reduce the risk of prostate cancer and other forms of cancer and cardiovascular disease. HPTLC on silica gel with petroleum ether – dichloromethane 9:1. Quantification by densitometry at 416 nm. Four products containing 300 µg, 3 mg, 5 mg, or 10 mg lycopene plus other ingredients were quantified using a lycopene standard: the measured amounts ranged from 77.7 to 98.1 % of the stated label values. The accuracy by spiked blank analysis was within 1.90 % of theoretical values for the 3 mg softgels and 1.10 % of theoretical values for the 10 mg softgels. The precision of replicate analyses showed a RSD of 1.44 % for the 10 mg softgels and 2.39 % RSD for the spiked blank for the 3 mg softgels. The results obtained for Lycopene standards available from two other companies showed 55.6, 57.6, and 20.0 % of the minimum amount expected from the stated label values.

Acta Chromatographica 20(4), 625-636 (2008). TLC of of aceclofenac, paracetamol, and chlorzoxazone on silica gel (prewashed with methanol) with toluene – 2-propanol – ammonia 10:10:1. Detection and quantification by densitometry at 274 nm. The hRf values of aceclofenac, paracetamol, and chlorzoxazone were 28, 72 , and 51, respectively. The linearity was in the range of 400–1400 ng/band for aceclofenac, 2–7 µg/band for paracetamol, and 1–3.5 µg/band for chlorzoxazone, with r=0.9995, 0.9993, and 0.9996, respectively. The recovery of aceclofenac was 99.5-100.4 %, for paracetamol 100.0-100.5 %, and for chlorzoxazone 99.4-99.8 %.

Planta Med. 75, 711-718 (2009). For many decades, planar chromatography has been used for the analysis of plants, in particular today in its most advanced form of HPTLC. The technique is e. g. used for the identification of medicinal plants and dietary supplements, and for the detection of adulteration and quantitative determination of marker substances. Reliable qualitative and quantitative results can be achieved based on suitable instrumentation and adequate methodological concepts. The manageability of the entire planar chromatographic process has improved. Integration of biological detection systems as well as hyphenation to mass spectroscopy has widened the applicability of planar chromatography as an important analytical technique. The introduction is followed by explanation of HPTLC, use of HPTLC in plant analysis, limitations, applications (identification, detection of adulteration and quantitation), and instrumentation (chromatogram development, documentation, detection and evaluation).