J. Chinese Trad. Patent Med. (Zhongchengyao) 17 (10), 13-14 (1995). TLC on silica with cyclohexane - acetone 7:3. Detection under 365 nm. Quantification of 3,4,5-trimethoxy-trans-ethyl cinnamate by densitometry at 365 nm.

Anal. Biochem. 242, 152-155 (1996). HPTLC of phosphatidylinositol 3,4,5-trisphosphospate, phosphatidylinositol 3,4-bisphosphosphate and phosphatidylinositol 4,5-bisphosphosphate on silica gel impregnated with 5% boric acid solution in methanol, and of phospatidylinositol 4-phosphate and phosphatidylinositol 3-phosphate on NH2 impregnated with 5% boric acid solution in methanol. Development with 1-propyl acetate - 2-propanol - ethanol - 6% NH3 1:3:1:3. Visualization by immersion in 5% CuSO4 solution in 8% aqueous H3PO4 and heating at 180°C for 15 min. Quantitative determination by autoradiography and by densitometry at 366nm.

J. Chromatogr. B 706, 362-366 (1998). TLC on silica gel with ethyl acetate - methanol - NH3 conc. 8:1:1. Quantitation by densitometry at 238 nm. Detection limit 20 mg and limit of quantitation 40 mg.

J. Chinese Trad. Patent Med. (Zhongchengyao) 20 (5), 11-13 (1998). TLC on silica gel with 1) chloroform - ethyl acetate - methanol - formic acid 400:50:100:2, 2) methanol - acetone - chloroform - NH3 6:8:26:1, 3) chloroform - methanol 5:1, 4) cyclohexane - ethyl acetate 1:1. Detection 1) by spraying with 5% vanillin-sulfuric acid reagent and heating at 105°C for 5 min, 2) by spraying with 0.5% ninhydrin in ethanol and heating at 105°C for 10 min. Identification by finger print technique. Quantitation of paeoniflorin by spectrophotometry at 230 nm after elution. Recovery 97.3 ± 1.1% (n=3).

CBS 90, 6-7 (2003). HPTLC phospholipids and glycolipids from rape seed on lichrospher silica gel with chloroform - methanol - acetone - water 18:15:2:1 for phospholipid separation and with acetone - chloroform - water 30:15:2 for glycolipid separation, developing distance 70 mm each. Detection by dipping in molybdatophosphoric acid reagent (5 % in ethanol) followed by heating at 120 °C for 15 min. Quantitative determination by absorbance measurement at 720 nm.

Analysis of choline esterase inhibitors. CBS 83, 4-5 (1999) HPTLC-AMD of choline esterase inhibitors on silica gel with a 15-step gradient from methanol via dichloromethane to hexane. Detection by spraying with butyryl choline esterase solution followed by incubation at 37 °C for 30 min, and spraying with fast blue salt mixed with alpha-naphthyl acetate followed by incubation at 37 °C for 5 min. Quantitative determination by absorbance measurement at 254 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (8), 627-629 (2004). TLC on silica gel 2-fold with petroleum ether - ethyl acetate 9:1. Detection 1) under UV 254 nm. Identification by comparison with the standards. Quantitation by densitometry at 300 nm. Validation of the procedure by investigation of its precision, specificity, repeatability, reproducibility, recovery, etc.

CBS 91, 12-13 (2003). HPTLC of flavonoids with tetrahydrofuran - toluene - formic acid - water 16:8:2:1, diterpenes with toluene - ethyl acetate 9:2, and iridoids with ethyl acetate - methanol - water 77:15:8 (with chamber saturation), in horizontal developing chamber over 52 mm. Detection of flavonoids by dipping warm plate in natural products reagent (0.5 % in ethyl acetate) followed by dipping in PEG 400 solution (5 % in dichloromethane), of diterpenes by dipping in 10 % methanolic sulfuric acid followed by heating at 105 °C, and iridoids by dipping in 4-dimethylaminobenzaldehyde reagent (1 % in 1 N methanolic HCl). Visual evaluation at 366 nm (flavonoids), white light (diterpenes), and white light with sharp cut filter 560 nm (iridoids). Stability of extracts was investigated under drastic stress conditions (acid, base, light, heat, humidity).