Food Chem. 95, 58-64 (2006). HPTLC of nicotinic acid (1) and pyrazole-3(5)-carboxylic acid (2) of Volvariella volvacea on silica gel with chloroform – methanol 17:3 with one drop of formic acid added. Quantitative determination by absorbance measurement at 190 nm for (1) and 262 nm for (2). The hRf values for (1) and (2) were 30 and 40, respectively. Linearity was between 400 and 7000 ng/zone (1) and 200 and 2500 ng/zone for (2). The limits of detection and quantification were 50 and 400 ng/zone for (1) and 20 and 200 ng/zone for (2). Recoveries of both compounds were between 96 and 102 %.

59th Indian Pharmaceutical congress E-243, 283, (2007). HPTLC of Bergenia ciliata leaves and rhizomes successively (Soxhlet) extracted with petroleum ether (40-60°C), chloroform, n-butanol, and ethyl acetate, on silica gel with ethyl acetate - glacial acetic acid - formic acid - water 128:50:50:122. Evaluation under UV 254 nm.

59th Indian Pharmaceutical Congress F-57, 403, (2007). HPTLC of duloxetine hydrochloride on silica gel with chloroform - methanol 4:1. Densitometric evaluation at 217nm. The hRf value of duloxetine was 45. The limit of detection and quantification was 120 and 240 ng/zone, respectively. Degradation products (acid, alkali, oxidative and thermal) were well separated from the main component. The proposed HPTLC method was routinely applied for identification and quantification of the drug in the formulation.

Chromatographia 66 (1-2), 95-102 (2007). HPTLC of dasatinib in the presence of its degradation products, on silica gel sheets with toluene - chloroform 7:3. Quantification by densitometry at 280 nm. The hRf value of dasatinib was 23 and selectivity regarding matrix was given. Validation of the method as per the ICH guidelines. Dasatinib was subjected to acid–alkali hydrolysis, oxidation, dry heat, wet heat and photodegradation. The drug was susceptible to acid–alkali hydrolysis and oxidation. The drug was found to be stable in neutral, wet heat, dry heat and photo-degradation conditions.

CBS 98, 2-4 (2007). HPTLC of amino-3-propan-1-ol, a degradation product of dexpanthenol, on silica gel with ethanol - water - acetic acid 16:3:1. To the mobile phase the derivatization reagent was added, i.e. 0.5 g ninhydrin were dissolved in 100 mL solvent mixture. Development in the horizontal developing chamber from both plate sides over 40 mm. Detection by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 486 nm. The hRf value of amino-propanol was 50. The mean repeatability was 4.9 % at 5 different concentration levels. The relative standard deviation of the intermediate precision (n=9) was 5.7 %. The limit of detection and quantification was 4.5 µg/mL and 15 µg/mL, respectively (related to the application volume of 2 µL). Recovery (n=15) was 102 %.

J. Planar Chromatogr. 20, 203-207 (2007).. HPTLC of epalrestat (5-[(1Z,2E)-2-methyl-3-phenylpropenylidene]-4-oxo-2-thioxo-3-thiazolidineacetic acid) with nitrofurantoin as internal standard on silica gel with ethyl acetate - toluene - acetic acid 30:20:1. Densitometric scanning in absorbance mode at 290 nm.

J. Planar Chromatogr. 20, 275-277 (2007). HPTLC of bergenin and gallic acid on silica gel with ethyl acetate - formaldehyde - acetic acid - water 80:1:2:1 in a saturated twin-trough chamber. Quantitation was performed by scanning at 260 nm in absorption mode.

Chromatographia 67 (3-4), 269-274 (2008). Presentation of a quantitative method using silica gel HPTLC plates, automated bandwise sample application, and automated visible mode densitomety for the determination of 24beta-ethylcholesta-5,22E,25-triene-3beta-ol (ECTO) in the aerial part of Clerodendrum phlomidis, which was used as a chemical marker for the standardization of C. phlomidis plant extracts. HPTLC on silica gel with chloroform - methanol 197:3. Detection by derivatization with anisaldehyde reagent. Quantitative determination by absorbance measurement at 650 nm. Linearity was between 150 and 400 ng/band with good correlation (r2 = 0.996).