J. Chromatogr. A 1529, 93-106 (2017). Presentation of the quantitative effect-directed profiles of 77 industrially and freshly extracted botanicals like herbs, spices, vegetables and fruits, widely used as food ingredients, dietary supplements or traditional medicine for their quality assessment with regard to potential health-promoting activities. Fast assignment of single active compounds and evaluation of their contribution to the overall activity, originating from a food or botanical sample by combination of HPTLC hyphenated with UV/Vis/FLD detection and effect-directed analysis, using the 2,2-diphenyl-1-picrylhydrazyl radical, Gram-negative Aliivibrio fischeri, Gram-positive Bacillus subtilis, acetylcholinesterase and tyrosinase assays. Characterization of bioactive compounds of interest eluted using an elution head-based interface by HPTLC-UV/Vis/FLD-EDA-ESI-(HR)MS method. Demonstration of the excellent quantification power of the method by applying for rosmarinic acid, contents ranged from 4.5 mg/g (rooibos) to 32.6 mg/g (rosemary), for kaempferol-3-glucoside from 0.6 mg/g (caraway) to 4.4 mg/g (wine leaves), and for quercetin-3-glucoside from 1.1 mg/g (hawthorn leaves) to 17.7 mg/g (thyme). The mean repeatabilities (%RSD, n=18) were ≤ 2.2 % for the three compounds and the mean intermediate precision (%RSD, n=3) was 5.2 % over three different days.

J. Chromatogr. Sci. 56 (1), 81-91 (2018). HPTLC of mixures of tamsulosin hydrochloride (TAM) with either tolterodine tartrate (TOL) or solifenacin succinate (SOL) in bulk drug and in combined dosage forms on on silica gel with ethyl acetate – methanol – ammonia 120:80:1. Quantification by densitometry at 224 nm. Linearity was between 0.1-0.7, 0.4-4 and 1-6 μg/band for TAM, TOL and SOL, respectively.

J. Chromatogr. Sci. 56, 317-326 (2018). Development of an accurate, sensitive and highly selective stability-indicating method for simultaneous determination of agomelatine (AGM) and its forced degradation products (Deg I and II) by HPTLC on silica gel with chloroform – methanol – ammonia 90:10:1. Quantitative determination by densitometry at 230 nm over the concentration range of 0.2-1.2 μg/band for AGM in pure form and human plasma, and 0.1-1 μg/band for both Deg I and II. The method was successfully applied for the analysis of AGM in pharmaceutical formulations. Statistical comparison of the results to those obtained by HPLC revealed its high accuracy and good precision.

varieties by analytical OPLC. Proc. Intern. Symposium on TLC with special Emphasis on OPLC, Szeged, 46 (1984). OPLC (OPTLC) of trimethyl-L-lysine on silica with propanol - methanol - 0.l M sodium acetate in water 20:3:30. Detection with Dragendorff reagent. Densitometry by absorbance at 525 nm.

J. Chromatogr. 291, 464-470 (1984). TLC of carbapenem antibiotics on silica with chloroform - methanol - 0.01 M phosphate buffer at pH 7.5 8:6:1. Detection by dipping the plate in Ehrlich reagent. Quantification by scanning by absorbance at 575 nm. Also bioautography with comamonas terrigena. Comparison of densitometry with bioautographic analysis.

J. High Resol. Chromatogr. 8, 126-131 (1985). HPTLC of carboxylic acids on cellulose with ethyl acetate - toluene - water - formic acid 60:20:20:15. Detection by staining with xylose - aniline reagent. Densitometry by absorbance at 546 nm. The method is quick, sensitive and has a wide dynamic range for the acids analyzed.

Budapest Chromatography Symposium, June 13, 1985. EGYT 1855: TLC on silica with chloroform in the first dimension, ethyl acetate - propanol -10 % acetic acid 2:2:1 in the second dimension. Staining with Dragendorff reagent; densitometry by absorbance at 450 nm. GYKI 51 189: TLC on silica with benzene in the first dimension, benzene - ethyl acetate 20:8 in the second dimension; detection by UV 254 nm, densitometry by absorbance at 300 nm.

Shenyang Pharm. Coll. (Shenyang Yaoxueyuan Xuebao) 1, 147-151 (1984). (Chinese) (Determination of curecumol in the volatile oil of curecuma aromatica by thin-layer chromatography.) TLC of curecumol on silica with petrol ether - ethyl acetate 95:5. Detection by spraying with 10 % vanillin in acetic acid - HClO4 15:1. Determination by densitometry.