62nd Indian Pharmaceutical Congress Abstract No. F-245 (2010). TLC of amlodipine besylate and nebivolol hydrochloride on silica gel with methylene chloride – methanol – 25 % ammonia 17:2:1. Both drugs were well resolved with hRf values of 19 and 41 for amlodipine besylate and nebivolol hydrochloride respectively. Quantitative determination by absorbance measurement at 285 nm. The method was linear in the range of 200-600 ng/band for both drugs. Recovery was 99.9-102.1 %.

62nd Indian Pharmaceutical Congress Abstract No. F-381 (2010). TLC of citicoline sodium on silica gel with chloroform – methanol – water 3:7:3. The hRf value was 53. Quantitative determination by absorbance measurement at 280 nm. The results of the method were comparable with the results of a RP-HPLC method.

J. Chem. Pharm. Res. 2(1), 155-161 (2010). A chroman derivative (C16O4H22) was isolated from the ethanolic extract of dried seeds of Ensete superbum. HPTLC on silica gel with toluene – ethyl acetate – formic acid 5:4:1. Quantitative determination by absorbance measurement at 254 nm. The linear range was 300-900 ng/band. The amount of the chroman in different fractions of the extract was 1.83 % (ethanol fraction), 1.74 % (ethyl acetate fraction) and 0.74 % (methanol fraction).

J. Chromatogr. A 1218 (19), 2745-2753 (2011). HPTLC of sucralose in waste water on silica gel with isopropyl acetate – methanol – water 15:3:1. The developing time was 15 min. Detection with p-aminobenzoic acid reagent. Quantification by absorbance measurement at 400 nm. The limit of quantification was 100 ng/L at a recovery rate of 80 % and the extraction of a 0.5 L water sample. An interlaboratory trial in 2008 showed good agreement of the sucralose content determined in four water samples by HPTLC and other methods (HPLC–MS/MS or HPLC–TOF-MS). The good accuracy and high sample throughput capacity proved HPTLC as a well suited method for quantification of sucralose in various aqueous matrices.

J. Planar Chromatogr. 24, 211-213 (2011). HPTLC of exo-polysaccharides from bacterial fermentation and a series of mono-, oligo-, and polysaccharide reference solutions on silica gel with chloroform - toluene - 35 % formic acid - methanol 10:2:2:7 in a twin-trough chamber saturated for 1 h. Detection of starch-type polysaccharides by placing the plate into a twin-trough chamber saturated with iodine vapor. After drying determination by densitometry in absorbance mode at 400 nm. The plates were then immersed for 1 min in a solution of 10 % concentrated sulfuric acid in n-propanol - toluene 1:1, followed by heating at 115 °C for 10 min. The zones were evaluated visually and densitometrically at 400 nm.

J. Planar Chromatogr. 24, 524-528 (2011). TLC of cefixime on silica gel with toluene - ethyl acetate - formic acid - water 5:29:11:5 with chamber saturation for 30 min at 25 +/- 2 °C. The hRf value was 54. Quantitative determination by densitometry in absorbance mode at 293 nm. Linearity was between 500 and 1500 ng. The repeatability (%RSD) was below 2 %. The limit of detection and the limit of quantification was 9 and 42 ng/zone, respectively. The recovery was between 98.9-100.3 %.

J. Planar Chromatogr. 24, 373-375 (2011). HPTLC of red clover capsule extracts and formononetin, biochanin A, daidzein, glycitein, and genistein on silica gel, prewashed with methanol, with dichloromethane - glacial acetic acid - ethyl acetate 12:2:1 in a horizontal chamber saturated for 15 min. Quantitative determination by densitometry at 260 nm. The hRf value was 29, 34, 41, 48, and 59 for daidzein, glycitein, genistein, formononetin, and biochanin A, respectively. The two major isoflavones are formononetin and biochanin A. The limit of detection and quantification was 14 and 47 ng/band for formononetin and 12 and 40 ng per band for biochanin A, respectively. The recovery was 93.3-100.7 % for formononetin and 102.0-109.4 % for biochanin A.

J. Planar Chromatogr. 24, 376-380 (2011). HPTLC of stem bark extracts from D. melanoxylon and lupeol and betulin on silica gel, prewashed with methanol, with ethyl acetate - hexane 9:41 with chamber saturation for 3 min at 29 +/- 4 °C and 65 +/- 5 % relative humidity. The hRf value was 46 and 25 for lupeol and betulin, respectively. Quantitative determination by densitometry in absorption mode at 560 nm for lupeol and 510 nm for betulin. Detection by derivatization with 5 % methanol-sulfuric acid reagent. The LOD and LOQ was 40 and 100 ng/zone for lupeol and 50 and 100 ng/zone for betulin, respectively. The instrument precision and repeatability (n = 6) were 0.8 and 1.3 % for lupeol and 1.1 and 1.2 % for betulin, respectively. The linearity range was 100-500 ng/zone for both lupeol and betulin. The intra-day and inter-day precision was 1.1-1.7 % and 1.3-2.0 % for lupeol and 0.8-1.9 % and 1.9-2.2 % for betulin.