J. Pharm. Biomed. Anal 36, 617-620 (2004) HPTLC of caffeine in herbal products and energy drinks, on silica gel with ethyl acetate - methanol 17:3. Solid samples (capsules) were extracted with methanol, filtered and applied whereas liquid samples (coca cola) were applied after the effervescence has ceased. Quantitative determination by absorbance measurement at 275 nm. The developed method was validated for specificity, repeatability (CV < 5 %), recovery (98.90) and accurary (99.84). The levels of caffeine were 4.76-13.29 % and 0.011-0.032 % for the herbal products and the energy drinks resp.

Indian Drugs 42 (6), 345-352 (2005). TLC fingerprint identification of methanolic extracts of Adhatoda vasica, Lawsonia inermis and Alkanna tinctoria, on silica gel. For Lawsonia inermis and Alkanna tinctoria the developing solvent toluene - acetone - acetic acid 90:10:1 was used, and for Adhatoda vasica n-hexane - acetone - diethyl ether 3:1:1 for. Detection of alkannin by spraying with 10 % methanolic KOH.

Indian Drugs 42 (7), 424-427 (2005) HPTLC of fexofenadine HCl from tablet dosage form on silica gel with dichloromethane - methanol 13:7. Quantitative determination by absorbance measurement at 260 nm. The linear detector response was observed between 0.2 and 1.0 µg. The method was validated to determine its accuracy and precision. The LOD was found to be 0.08 ng/µL, LOQ was 0.02 ng/µL. The recovery was carried out by standard addition method and was found to be 100.82 %.

Acta Chrom. 11, 102-107 (2001). The snail Biomphalaria glabrata is medically important because it serves as the intermediate host for the development and transmission of the human blood fluke parasite Schistosoma mansoni. The purpose of this study was to use HPTLC to determine the effects of S. mansoni larval infection on the maltose and glucose content of the hemolymph and digestive gland–gonad complex of B. glabrata. TLC on silica gel with pre-adsorbent zone and 19 channels, with ethyl acetate – acetic acid – methanol – water 13:3:3:2. Detection by spraying with 1-naphthol – sulfuric acid reagent, quantitative determination by absorbance measurement at 515 nm.

J. Sep. Sci. 29, 2716-2724 (2006). HPTLC of alfuzosin hydrochloride subjected to stress conditions (alkaline, acidic, oxidative, thermal, and photo-degradation) on silica gel with methanol - ammonia 125:3. Quantitative determination by absorbance measurement at 245 nm. Linearity of determination of alfuzosin is between 0.5 and 7.0 µg and its average percentage recovery is 98.5 - 101.8 %. The drug is well separated from its degradation products upon applying the two methods.

Acta Chrom. 12, 143-150 (2002). HPTLC of caffeine in pharmaceutical preparations on silica gel with ethyl acetate – methanol 17:3. Densitometry at 275 nm. Tablet, coated tablet, and coated caplet products containing caffeine as the active ingredient were analyzed to test the applicability of the new method.

J. Liq. Chromatogr. & Relat. Technol. 29, 2997-3007 (2006). TLC of pyrocatechol on silica gel (prewashed with methanol - chloroform 1:1), with methanol - chloroform 1:9. Densitometric measurement at 200 nm. Investigation of stability on silica gel, as well as in solutions, in relation to different storage conditions.

Indian J. Pharm. Sci. 68 (6), 793-796 (2006). HPTLC of atorvastatin calcium and ezetimibe in combined dosage form on silica gel with chloroform - benzene - methanol - acetic acid 60:30:10:1. Detection under UV 250 nm.The method was validated in terms of linearity, accuracy, precision and specificity.The calibration curve was found to be linear between 0.8 and 4.0 µg/spot for atorvastatin calcium and 0.1 and 1.0 µg/spot for ezetimibe. The limit of detection and the limit of quantification for atorvastation calcium were found to be 170 ng/spot and 570 ng/spot, respectively, and for ezetimibe 20 ng/spot and 70 ng/spot, respectively.The recovery was in the range of 99.9 - 102.7 %.