J. Liq. Chrom. & Rel. Technol. 24, 1467-1478 (2001). HPTLC of neutral lipids (with free fatty acids, free sterols, triacylglycerols, methyl esters, cholesteryl esters as standards) on silica gel with petroleum ether - diethyl ether - acetic acid 40:10:1, preequilibrated for 15 min. Detection by spraying with 5% ethanolic phosphomolybdic acid, and heating 15 min at 115°C. Quantification by densitometry at 700 nm. HPTLC of phospholipids (with cholesterol, phosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine as standards) with chloroform - methanol - water. Detection by spraying with a 10% solution of copper sulfate in 8% phosphoric acid and heating at 160°C for 10 min. Quantification by densitometry at 370 nm. TLC of lipophilic pigments (with b-carotene and lutein as standards) on RP-18 with petroleum ether (35-60°C) - acetonitrile - methanol 1:2:2. Detection in visible light and quantification by densitometry at 448 nm for lutein and 455 nm for b-carotene. Quantitative TLC of carbohydrates on silica gel with ethyl acetate - acetic acid - methanol - deionized water, double development. Detection by spraying with 1-naphthol - sulfuric acid reagent and quantification by densitometry at 515 nm.

CBS 90, 2-4 (2003). HPTLC-AMD on silica gel with a 11-step gradient with chloroform - ethanol - acetone followed by 3 isocratic steps with chloroform for separation of cholesterol, cholesterol sulfate and various ceramide classes. For separation of cholesterol, fatty acids, triacylglycerol, cholesteryl esters, and squalene a 2-step gradient with n-hexane - ethyl acetate followed by an isocratic step with n-hexane. Conditioning between single runs with 4 M acetic acid. Detection by dipping in copper sulfate reagent followed by heating at 150 °C for 20 min. Quantitative determination by absorbance measurement at 546 nm.

Juss) as determined by a combination of chromatographic and spectral techniques. J. Liq. Chromatogr. & Relat. Technol. 30,11-25 (2007). TLC on silica gel with petroleum ether - acetone 25:2; detection by spraying with 50 % ethanolic sulfuric acid and heating at 200°C. Isolation, purification and quantification of lipid classes by preparative TLC; detection by spraying the edges with 2’,7’-dichlorofluorescein for visualization under UV. Preparative separation of acylglycerols, free fatty acids, and polar lipids on silica gel with petroleum ether - acetone - acetic acid 70:30:1. Quantitative Ag-TLC on silica gel impregnated by dipping into a 0.5 % methanolic silver nitrate solution - also preparative Ag-TLC. Quantitative TLC on RP by densitometry at 450 nm. Ag-TLC provided the quantitative data for the TAG classes differing in unsaturation, and RP-TLC for the TAG species differing in chain length within a given class.

J. Lipid Res. 38, 1482-1488 (1997). TLC of phospholipids and neutral lipids on EDTA-impregnated silica gel and after pre-concentration with chloroform - methanol - water 60:40:10 with five step-wise developments: i) chloroform - methanol - water 65:40:5 to 2 cm, ii) ethyl acetate - 2-propanol - ethanol - chloroform - methanol - 0.25% KCl 35:5:20:22:15:9 to 5 cm, iii) toluene -diethyl ether - ethanol 60:40:3 to 7.5 cm, iv) n-heptane - diethyl ether 94:8 to 10.5 cm, v) pure n-heptane to 12.5 cm. Charring by dipping in a solution of 10% cupric sulfate in 8% phosphoric acid for 10 s and heating at 200°C for 2 min. Quantitative determination with an image analyzer in transmission mode.

J. Planar Chromatogr. 30, 460-466 (2017). HPTLC of cholesterol oleate, glycerol trioleate, squalene, β-sitosterol, linoleic acid, glycosylsterol (β-sitosterol glucoside), glycosylceramide, protocatechuic acid and quercetin 4ʹ-O-β-d-glucopyranoside (2) on silica gel with chloroform ‒ methanol 17:3. Detection by dipping into a solution of copper(II) sulfate (10 %), phosphoric acid (8 %), and methanol (5 %) in water, followed by heating at 150 °C for 10 min. Quantitative determination by absorbance measurement at 546 nm. Linearity was between 25 and 1000 ng/zone. LOD and LOQ were between 50 and 125 ng/band and 125 and 250 ng/band, respectively. The intermediate precision was <2 % (n=6). Recovery ranged from 106.5 % to 112.1 %.

Anal. Biochem. 227, 195-200 (1995). TLC mapping of the title compounds on NH2-bonded silica with chloroform - methanol - 1% diethylamine 50:47:15 for the 1st dimension, transporting the acid glycosphingolipids on this plate to a silica plate by developing with chloroform - methanol - NH3 2:5:3 after NH2-plate placed face to face on top of silica plate, and developing the plate with chloroform - methanol - 0.2% CaCl2 6:4:1 for the 2nd dimension. With this method it is possible to compare detailed membrane constituents, including minormolecular species with regard to acidic glycosphingolipids.

Giovanni Olini (1200 AD) and St. Nicolosa Bursa (1500 AD). J. Planar Chromatogr. 28, 205-212 (2015). TLC of (1) proteins, (2) resins, (3) sugars, and (4) waxes from fragments of the surface-coating material found on mummified bodies on silica gel for (2), (3), and (4), and on cellulose phase for (1) with n-butanol - acetic acid - water 4:1:1 for (1), benzene - methanol 19:1 for (2), acetonitrile - water 17:3 for (3), and petroleum ether - diethyl ether - acetic acid 90:10:1 for (4). Detection was performed after derivatization with ninhydrine reagent for (1), and iodine reagent for (2), (3) and (4). The combination of TLC and other chemical methods proved to be an effective and low-cost tool for obtaining valuable information during the archaeological investigation.

Clin. Chim. Acta 118, 109-120 (1982). TLC of phosphatidylglycerin on silica (containing 5 % ammoniumsulfate) with 1a) THF- dimethoxymethane -methanol - NH3 30:20.6:5.6:3, 1b) chloroform - methanol 60:9.