Biochem. Biophys. Res. Commun. 536, 73-79 (2021). HPTLC of ceramide dihexosides (lactosylceramide and its structural isomer, galabiosylceramide) in human cerebrospinal fluid on silica gel with chloroform - methanol - water 65:25:3. Detection by spraying with orcinol reagent, followed by heating at 120 ºC for 10 min.

Biochem. Biophys. Res. Commun. 525, 61-66 (2020). HPTLC of complete precursors of glycosylphosphatidylinositol (GPI) in Gpi8 deficient strains of Candida albicans on silica gel with chloroform - methanol - water 4:4:1. Metabolic labeling was performed using [2-3H]-myo-inositol. Detection of radiolabeled glycolipid intermediates by scanning for 5 min.

J. Sep. Sci. 43, 1566-1575 (2020). HPTLC of phenyl myristate in the bark and leaves of Homalium nepalense on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 254 nm. The hR_F value for phenyl myristate was 49. Linearity was in the range of 100-500 ng/5 µL. Intermediate precisions were below 1 % (n=5). The LOD and LOQ were 3 and 10 ng/5 µL, respectively. Average recovery was  between 90.1 and 95.6 %.

Rapid Commun. Mass Spectrom. 33, 60-65 (2019). TLC of oxidation products of 1‐palmitoyl‐2‐oleoyl‐sn‐phosphatidylcholine (POPC) and 1‐palmitoyl‐2‐linoleoyl‐sn‐phosphatidylcholine (PLPC) on silica gel (NP) and RP-18 with chloroform - ethanol - water - triethylamine 20:35:7:35 for NP-TLC and methanol - chloroform - water 10:1:1 for RP-TLC. Detection for NP-TLC under UV 366 nm after spraying with primuline, and for RP-TLC under white light after spraying with molybdatophosphoric acid solution, followed by heating at 130 ºC. Lipids zones from both plates were eluted with methanol and further analyzed by electrospray ionization mass spectrometry.

J. Food Compos. Anal. 77, 39-43 (2019). TLC of marine oil omega-3 supplements on silica gel with hexane - diethyl ether - glacial acetic acid 50:10:1. Detection by spraying with anisaldehyde solution, following by heating using a heat gun. Analysis of samples allowed the identification of different sources of omega-3 polyunsaturated fatty acids (PUFA) in their natural triacylglycerol (TAG) or concentrated ethyl ester (EE) forms.

J. Liq. Chromatogr. Relat. Technol. 42, 1-8 (2019). HPTLC of phospholipids  (phosphatidylcholines, phosphatidylethanolamines, cardiolipins and phosphatidylglycerols) associated to membrane proteins in Rhodobacter (Rb.) blasticus, Rhodospirillum (R.) rubrum and Rhodobaca (Rbc.) bogoriensis on silica gel with a 7-step gradient based on methanol - water - ethyl acetate. HPTLC was coupled to electrospray mass spectrometry (ESI-MS) using an elution head-based interface for the identification of several phospholipid species.

J. Liq. Chromatogr. Relat. Technol. 42, 249-257 (2019). HPTLC of methanolic extracts from the leaves of Paulownia tomentosa on silica gel with chloroform - ethyl acetate - methanol 20:3:2. HPTLC-direct bioautography by dipping into B. subtilis cell suspension, followed by incubation at 28 °C for 2 h. Then the bioautograms were dipped into an aqueous solution of the MTT vital dye (1 mg/mL (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide), followed by incubation at 28 °C for 30 min. Further analysis by using a HPLC-DAD-MS system allowed the identification of apigenin and p-coumaric acid as highly abundant antibacterial components.

Planta Med. 83(17), 1321-1328 (2017). Benzoyl-aconine esters (lipo-alkaloids) produced by transesterification of aconitine (isolated from Aconitum sp.) with long-chain fatty acids were purified by a multistep chromatographic method, including cyclodextrane gel filtration chromatography, centrifugal planar chromatography on aluminium oxide layer using cyclohexane – chloroform – methanol 70:30:1 followed by 70:30:3 and/or preparative thin-layer chromatography on aluminium oxide layer with toluene – acetone – ethanol – concentrated ammonia 70:40:10:3.