Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
- Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
- Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.
Microbiol. Res. 166, 255-267 (2011). TLC of lipopeptides (produced by Pseudomonas species in cultures of Pleurotus ostreatus; Pseudomonas reactans was used as a reference) on silica gel with chloroform – methanol – ammonia 80:25:4. Detection by spraying with 0.1 % bromothymol blue in ethanol, followed by heating.
Pharm. Res. 33, 1587-1601 (2016). HPTLC study of the cleavability of the disulfide bond in a conjugate siRNA-S-S-PE (incubated in 10 mM glutathione at 37 °C for 4 h) on silica gel with chloroform – methanol 4:1. Detection by spraying with molybdenum blue dye for the cleaved phospholipids. This test confirmed the potential utility of this system for siRNA delivery in vivo.
J. Agric. Food Chem. 33, 1093-1096 (1985). Two-dimensional TLC of radioactive total polar lipids on silica using chloroform - methanol - water 65:25:4 and chloroform - methanol - NH3 65:25:5 as eluents. Visualization with Dragendorff and ninhydrin reagents, also radioscanning.
I. Separation and identification of prostaglandin A1 from the common onion, Allium cepa.) (Chinese). J. Chinese Herb. Med. 19, 146-147 (1988) (Zhongcaoyao). Preparative TLC on silica with chloroform - THF - acetic acid 10:2:1. Elution with chloroform - methanol 1:1. Identification by UV, IR, NMR, MS.
J. Planar Chromatogr. 2, 24-27 (1989). HPTLC of glycerylmonostearate on silica with hexane - ethyl acetate - methanol 7:2:1. Postchromatographic derivatization by dipping into 0.05% methanolic solution of 6-(p-toluidino)-2-naphtalene-sulfonic acid (potassium salt) and then under UV 365 nm. HPTLC of glyceryl monodiricinolate on silica with ethyl acetate - dichloromethane - methanol - formic acid 13:6:1:0.1. Post-chromatographic derivatization by dipping into 3% ethanolic solution of molybdato phosphoric acid and heating to 120°C for 15 min.
J. Lipid Res. 38, 1482-1488 (1997). TLC of phospholipids and neutral lipids on EDTA-impregnated silica gel and after pre-concentration with chloroform - methanol - water 60:40:10 with five step-wise developments: i) chloroform - methanol - water 65:40:5 to 2 cm, ii) ethyl acetate - 2-propanol - ethanol - chloroform - methanol - 0.25% KCl 35:5:20:22:15:9 to 5 cm, iii) toluene -diethyl ether - ethanol 60:40:3 to 7.5 cm, iv) n-heptane - diethyl ether 94:8 to 10.5 cm, v) pure n-heptane to 12.5 cm. Charring by dipping in a solution of 10% cupric sulfate in 8% phosphoric acid for 10 s and heating at 200°C for 2 min. Quantitative determination with an image analyzer in transmission mode.
Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 97-105. HPTLC of Venticute on silica gel with chloroform - methanol - acetic acid - water 65:20:8:5. Visualization with 10% copper sulfate in 4% phosphoric acid. Quantification by absorbance measurement at 365 nm. Intermediate precision 1-4%, recovery 98-103%, limit of quantification 0.1-0.4% (ca. 15-40 µg/spot). Immuno TLC on diol plates with chloroform - methanol - 8% NH3 65:30:6. Immunological staining according to conventional western plot. Visualization via enhanced chemiluminescence detection system. Densitometric evaluation via peak area of the x-ray film including background subtraction. Recovery 65-120% and 75-110% (inter-assay, n=6, 3 concentrations), repeatability 24-28% and intermediate precision 22-52% (n=6, 3 concentrations), detection limit 0,125 ng HCP (host cell derived proteins), quantitation limit 0,02% and specification limit 0,1%. This HPTLC procedure can also be used to separate the hydrophobic protein from impurities and modification (which can not be separated by HPLC). Visualization via coommassie brilliant blue (0,15 %). Repeatability ca. 10% (n=8).