Scholars Research Library 2(3), 163-167 (20100. TLC on silica gel with n-butanol - water - methanol - ammonia 8:1:1:2 with chamber saturation for 10 min. The hRf value was 33 for gatifloxacin and 89 ambroxol HCl. The method was linear in the range of 100-600 ng/band for gatifloxacin and 12.5-62.5 ng/band for ambroxol HCI. The recovery was 99.3-100.3 %. Densitometric evaluation at 310 nm.

62nd Indian Pharmaceutical Congress Abstract No. F-332 (2010). TLC of ramipril and hydrochlorothiazide on silica gel with ethyl acetate – methanol – chloroform – glacial acetic acid 11:3:7:2. The hRf values were 28 and 49 for ramipril and hydrochlorothiazide,respectively. Quantitative determination by absorbance measurement at 210 nm. The method was linear in the range of 500-1900 ng/band for both compounds. The recovery was 98-102 % for both drugs. The results were comparable when the sample was analysed by a dual wave-length method. The proposed method can be used for analysis of formulation without any interference from excipients.

leaves and its geographical variation using HPTLC fingerprint. Trends in Natural Product Research, NRP-2011 abstract No. SNP-NRP-11/069. TLC of ferulic acid in ethanolic extracts of Ricinus communis leaves on silica gel with chloroform - methanol 19:1. Quantitative determination by densitometry at 366 nm. The method was linear in the range of 300-900 ng/band. LOD and LOQ were 4 and 11 ng/zone, respectively. The plant collected from different geographic locations showed variations in the amount of ferulic acid. The content of ferulic acid was 2.87 µg/g in leaves collected from Delhi, which was higher than those from Guwahati and Jhansi.

J. Planar Chromatogr. 27, 174-180 (2014). HPTLC of betulinic acid (1), 24beta-ethylcholesta-5,22E,25-triene-3beta-ol (2) and lupeol (3) in Clerodendrum phlomidis on silica gel with chloroform - methanol 49:1. Detection by dipping into vanillin - sulfuric acid reagent (vanillin - ethanol - sulfuric acid 1:95:5, w/V/V), followed by heating at 110 ºC for 3 min. Quantitative determination by absorbance measurement at 600 nm. The hRF values for (1) to (3) were 30, 52 and 70. Linearity was in the range of 300-1500 ng/mL. The intermediate/interday/intra-day precisions were below 2 % (n=5). The LOD and LOQ were 22 and 74 ng/zone for (1), 20 and 66 ng/zone for (2) and 30 and 100 ng/zone for (3), respectively. Average recovery was 99.0 % for (1), 98.5 % for (2) and 98.2 % for (3).

Anal. Chem. 56, 1035-1037 (1984). Improved detection system suitable for DEAE-cellulose in formic acid media. The new reagent is 1 or 2 orders of magnitude more sensitive than Ni(II)-DMG depending on the type of chelants. The 7 chelants identified were iminodiacetic acid, nitrilotriacetic acid, N-hydroxy-ethylenediamine-N,N',N-triacetic acid, ethylenediamimetetraacetic acid, (glycol ether) diamine-N,N,N',N'-tetraacetic acid, trans-1,2-cyclo-hexanediamine-N,N,N',N-tetraacetic acid, and diethylene-triamine-N,N,N',N-pentaacetic acid.

J. Agric. Food Chem. 34, 1016-1018 (1986). TLC determination of linoleic acid and reaction products (peroxides, ketodienes) on silica with petrol ether - ether - acetic acid 80:20:1. Visualization by spraying with 5 % ethanolic phosphomolybdic acid and heating at 110 °C. Detection of peroxides and ketodienes by spraying with ferrous thiocyanate and 2,4-dinitrophenyl hydrazine, respectively.

Chromatographia 23, 432-434 (1987). Discussion of TLC of carboxylic acids on barium sulfate - calcium sulfate in different proportions with various solvents.

(Japanese). Shimadzu Review 44, 243-246 (1987) (Shimazu Hyoron). TLC after esterification with G-anthryldiazomethane on silica with benzene - acetone system and on chemically bonded silica with methanol. Quantification by fluorodensitometry.