J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (9), 771-773 (2004). TLC on silica gel with isooctane - dibutyl ether - glacial acetic acid 8:5:5. Detection by spraying with 10 % H2SO4 in ethanol and heating at 105 ºC. Identification by standard comparison. Quantification of cholic acid by densitometry.

J. Planar Chromatogr. 19, 200-203 (2006). TLC of 18 phenolic acids (salicylic, m-hydroxybenzoic, p-hydroxybenzoic, protocatechuic, alpha-resorcylic, beta-resorcylic, gallic, vanillic, syringic, gentisic, veratric, cinnamic, o-coumaric, m-coumaric, p-coumaric, caffeic, ferulic, and sinapic acid) an aminopropyl silica gel, prewashed with methanol and acetone, in a horizontal DS-chamber with mobile phases comprising mixtures of diisopropyl ether and acetic acid with toluene, petroleum ether, or heptane, partly with two developments. The best separations were obtained with heptane - diisopropyl ether - acetic acid 4:5:1, or petroleum ether - diisopropylether - acetic acid 6:3:1. Detection under UV light at 254 or 366 nm.

Chem.) 34, 707-711 (l985). (Japanese) (Determination of higher fatty acids in phospholipid subfractions of human platelets using thin-layer chromatography and gas chromatography.) TLC of phospholipid subfractions on silica with chloroform - methanol acetic acid - formic acid 50:30:4.5:6.5. Detection by spraying with 8-anilinonaphtalenesulfonic acid ammonium salt. Quantification by GC after extraction and methylation. Phospholipids were extracted from silica with chloroform - methanol 1:4 once and methanol twice. Methylation was done with boron trifluoride and methanol. The method is highly reliable.

J. Liq. Chromatogr. Relat. Technol. 30, 2267-2285 (2007). Analytical and preparative TLC of lipid classes, their fatty acid profiles, and the triacylglycerol and sterol composition on silica gel and modified silica gel (impregnated with silver nitrate for Ag-TLC or dimethyldichlorosilane for RP-TLC). TLC of lipid reference mixture on silica gel with hexane - acetone 25:4. Detection by spraying with 50 % ethanolic sulfuric acid and heating at 200 °C. Preparative TLC for isolation and quantification, followed by detection under UV light, spraying the edges with 2’,7’-dichlorofluorescein, scraping off, elution with diethyl ether and weighting. Quantitative Ag-TLC (impregnated by dipping into 0.5 % or 2 % methanolic solution of silver nitrate) followed by detection with bromine and sulfuryl chloride vapor for 30 min each, followed by heating at 180-200 °C. Preparative Ag-TLC with 4 different mobile phases. Quantitative RP-TLC on Kieselguhr treated for 6 h with vapors of dimethyldichlorosilane and washed with methanol using acetone - acetonitrile - water. Quantitative determination by absorbance measurement at 450 nm.

J. Planar Chromatogr. 26, 202-208 (2013). HPTLC of egg yolk lipid fractions on silica gel with hexane - diethyl ether - formic acid 40:10:1. Detection by srpaying with 10 % copper(II) sulfate in 8 % phosphoric acid. Quantitation by scanning with a flatbed scanner and a gel analysis software. Intermediate/interday/intra-day precision was below 10 % CV (n=6).

Marine Drugs 11 (2), 363-376 (2013). Lipid A fraction was isolated from Escherichia coli strain W3110 and from its plasmid-transformed derivatives HW000, HW001 and HW002 through an adapted three-step version of the Bligh and Dyer’s method. To control the transformation, TLC of the lipid extract, dissolved in chloroform – methanol 4:1, on silica gel with chloroform – methanol – water – ammonia 40:25:4:2. Detection by spraying with 10 % sulfuric acid in ethanol and heating at 175 °C; lipids were visualized as dark zones. Lipid A (hRf 40) was well separated from monophosphoryl lipid A (MPLA) (hRf 60) and from pentaacylated MPLA (hRf 50)._x000D_

Separations 5, 1-11 (2018). HPTLC of lipid content in yeast (clonal prion-infected and prion-free cells) on silica gel with 1) petroleum ether – diethyl ether – glacial acetic acid 80:20:1 for free sterols, free fatty acids, and triacylglycerols, 2) hexane – petroleum ether – diethyl ether – glacial acetic acid 50:20:5:1 for steryl esters, methyl esters, and squalene and 3) chloroform – diethyl ether – acetic acid 130:50:9 for phospholipids (phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol). Detection of neutral lipids by spraying with 5 % phosphomolybdic acid in ethanol, followed by heating at 120 °C for 30 min. Detection of phospholipids by spraying with 10 % cupric sulfate in 8 % phosphoric acid, followed by heating at 140 °C for 30 min. Evaluation at 370 nm (deuterium lamp) for phospholipids and at 610 nm (halogen-tungsten lamp) for neutral lipids. The hRf values for neutral lipids were 10 for cholesterol, 33 for oleic acid and 51 for triolein as well as 41 for methyl oleate, 56 for cholesteryl oleate and 77 for squalene. The hRf values of phospholipids were 21 for phosphatidylinositol, 27 for phosphatidylethanolamine and 48 for phosphatidylcholine. HPTLC demonstrated to be a powerful tool for revealing subtle changes in the physiology of yeast.

Proc. Intern. Symposium on TLC with special Emphasis on OPLC, Szeged, 109, (1984). TLC of neutral lipids on silica with petrol ether - ether - acetic acid 85:15:1 and two-dimensional TLC of phospholipids on silica with 1) chloroform - methanol - water 65:25:4; 2) diisobutylketone - acetic acid - water 8:5:1. Detection with iodine vapors for neutral lipids, with iodine vapors or ammonium molybdate-perchloric acid reagent for phospholipids.