J. Chromatogr. 483, 369-378 (1989). TLC and circular reversed-phase TLC of ethyl- and 2-chloroethyl esters of fatty acids. and cholesterol esters on silver nitrate-impregnated silica, and on octadecyl-bonded silica with various solvent systems. Detection by spraying with copper(II)sulfate reagent and heating at 100-120°C for 5 min., or by exposure to iodine vapor.

J. Chromatogr. 498, 423-427 (1990). TLC of neutral lipids on silica with 1) ether – benzene – ethanol – triethylamine 40:50:2:1, 2) ether – hexane – triethylamine 10:90:1, 3) ether – hexane – acetic acid 75:25:2 for the tree steps of development. Detection by charring with 95% sulfuric acid.

J. Agric. Food Chem. 39, 451-457 (1991). 2D-TLC of phosphatidyl choline, p-ethanolamine, p-inositol, p-serine, sphingomyelin, cardiolipin, lysophosphatidylcholine and lysophosphatidylethanolamine on silica impregnated with 7.5% magnesium acetate with chloroform - methanol - NH3 5:25:5 (1st dimension) and chloroform - acetone - methanol - acetic acid - water 6:8:2:2:1. Visualization by exposure to iodine vapor, comparison against standard mixture. Quantification after elution using perchloric acid digestion method. TLC analysis of fatty acids acc. to same method; scraping of phospolipids, transmethylation with BF3-methanol in benzene and GC. Thiobarbituric acid assay after TLC.

Chinese Anal. Chem. (Fenxi Huaxue) 19, 733-736 (1991). Two-dimensional TLC of phospholipids on silica with chloroform – methanol – NH3 (28%) 13:7:1 for the first dimension, and chloroform – acetone – methanol – acetic acid 10:4:4:2:1 for the 2nd. Detection by densitometry at 700 nm, and by 31P NMR. Comparison of the two methods.

J. Chromatogr. 615, 142-147 (1993). HPTLC of lipids on silica with 1) chloroform - ethanol - triethylamine - water 30:34:30:8, and 2) hexane ether 50:5 as the initial and final mobile phases, respectively. Detection by spraying with 10 -% copper sulfate in 8% phosphoric acid, and heating to 120 °C. Quantification by densitometry at 310 nm. Detection limit 20 ng/spot.

Biochemical Systematics and Ecology 23, 201-207 (1995). TLC of lipids on silica with hexane - ether - acetic acid 90:10:1. Visualization by spraying with 2,7-dichlorofluorescein solution in ethanol and under UV 360 nm. Purification of the sterol fraction by preparative TLC on AgNO3 - silica 2:8. GC analysis.

Chinese J. Pharm. Ind. (Zhongguo Yiyao Gongye Zazhi) 27, 222-224 (1996). TLC on silica with chloroform - methanol - ethanol - acetic acid - water 50:16:4:12:1. Detection by exposure to iodine vapor. Quantification by densitometry at 385 nm. Precision 3.56% (n=6). 97.7 ± 3.93% (n=6).

J. Planar Chromatogr. B, 155-158 (1999). HPTLC of neutral lipids (cholesteryl oleate, methyl oleate, triolein, oleic acid, and cholesterol) on silica gel with petroleum ether - diethyl ether - acetic acid - 40:10:1. To test for the presence of cholesteryl esters, plates were developed with n-hexane - petroleum ether - diethyl ether - acetic acid 50:20:5:1. All developments were performed at 20°C and 60% relative humidity in a vapor-equilibrated, paper-lined chamber. Neutral lipids were detected as blue zones on a yellow background by spraying with 5% ethanolic phosphomolybdic acid and drying in an oven at 110°C for 15 min. Densitometry of neutral lipid zones at 700 nm. TLC of phospholipids on silica gel with chloroform - methanol - water 65:25:4; detection as black zones on a white background by spraying with 10% copper(II) sulfate in 8% phosphoric acid reagent and heating at 140°C for 15 min. Quantitation by densitometry at 370 nm.