J. Planar Chromatogr. 6, 307-312 (1993). Part of this paper is the comparison of TLC-FID with classical TLC. Two-dimensional TLC of lipid extracts (e.g. diphosphatidylglycerides, phosphatidylglycerides, phosphatidylethanolamine, phosphatidylcholine) on silica with chloroform - methanol - 28% NH3 13:5:1 and chloroform - acetone - methanol - acetic acid - water 6:8:2:2:1. Visualization by exposure to iodine vapor. After elution determination by gravimetry.

J. Planar Chromatogr. 8, 475-479 (1995). HPTLC of phospholipid hydroperoxides and their parent phospholipids (e. g. sphingomyelin, phosphatidylcholine, phosphatidylethanolamine) on silica with hexane - ether 3:2 for the removal of all neutral lipids; HPTLC of phospholipids and PLHP with chloroform - ethanol - methanol - triethylamine - water 30:25:10:35:8. Detection by dipping in a freshly prepared solution of 1.00 g N,N-dimethyl-p-phenylenediamine and 1 mL of acetic acid in 50% aqueous methanol. After immersion for 60 s and drying for 60 min at 30 °C quantification by densitometry at 654 resp. 547 nm. Simple quantitative HPTLC method.

Proc. 9th Internat. Symp. Instr. Chromatogr., Interlaken, April 9.-11., 341-351 (1997). TLC and HPTLC of rodenticides and lipids for identification or characterization of feed source; HPTLC of rodenticides (ANTU, brodifacoum, bromadiolone, chlorophacinone, crimidine, coumachlor, coumatetralyle, difenacoum, pindone, pyranocoumarine, warfarin) on silica with hexane - ethyl acetate 7:3 and on RP-18 silica with methanol - water - acetic acid 75:25:0.6 and methanol - ammoniumacetic-triethylamine buffer 4:1. Quantification by densitometry at 310 nm (absorbance). TLC of lipids acc. to U. de la Vigne, D.E. Jänchen, Internat. Lab., 22-29 (1991).

J. Lipid. Res. 38, 1702-1706 (1997). Two-dimensional TLC of 1,2- and 1,3-diacylglycerols, and of ceramides containing a-hydroxy and normal fatty acids, on silica gel in the first direction with 1) chloroform - methanol 10:1. In the second direction development with 2) hexane - ethyl ether - acetic acid 80:20:1, and after drying once more with 3) heptane - diisopropyl ether - acetic acid 15:10:1. Visualization with Dittmer and Lester reagent. For liquid scintillation counting the radioactive spots were visualized by exposure to iodine vapor, and scraped into counting vials.

J. Liq. Chrom. & Rel. Technol. 24, 1467-1478 (2001). HPTLC of neutral lipids (with free fatty acids, free sterols, triacylglycerols, methyl esters, cholesteryl esters as standards) on silica gel with petroleum ether - diethyl ether - acetic acid 40:10:1, preequilibrated for 15 min. Detection by spraying with 5% ethanolic phosphomolybdic acid, and heating 15 min at 115°C. Quantification by densitometry at 700 nm. HPTLC of phospholipids (with cholesterol, phosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine as standards) with chloroform - methanol - water. Detection by spraying with a 10% solution of copper sulfate in 8% phosphoric acid and heating at 160°C for 10 min. Quantification by densitometry at 370 nm. TLC of lipophilic pigments (with b-carotene and lutein as standards) on RP-18 with petroleum ether (35-60°C) - acetonitrile - methanol 1:2:2. Detection in visible light and quantification by densitometry at 448 nm for lutein and 455 nm for b-carotene. Quantitative TLC of carbohydrates on silica gel with ethyl acetate - acetic acid - methanol - deionized water, double development. Detection by spraying with 1-naphthol - sulfuric acid reagent and quantification by densitometry at 515 nm.

CBS 90, 2-4 (2003). HPTLC-AMD on silica gel with a 11-step gradient with chloroform - ethanol - acetone followed by 3 isocratic steps with chloroform for separation of cholesterol, cholesterol sulfate and various ceramide classes. For separation of cholesterol, fatty acids, triacylglycerol, cholesteryl esters, and squalene a 2-step gradient with n-hexane - ethyl acetate followed by an isocratic step with n-hexane. Conditioning between single runs with 4 M acetic acid. Detection by dipping in copper sulfate reagent followed by heating at 150 °C for 20 min. Quantitative determination by absorbance measurement at 546 nm.

Juss) as determined by a combination of chromatographic and spectral techniques. J. Liq. Chromatogr. & Relat. Technol. 30,11-25 (2007). TLC on silica gel with petroleum ether - acetone 25:2; detection by spraying with 50 % ethanolic sulfuric acid and heating at 200°C. Isolation, purification and quantification of lipid classes by preparative TLC; detection by spraying the edges with 2’,7’-dichlorofluorescein for visualization under UV. Preparative separation of acylglycerols, free fatty acids, and polar lipids on silica gel with petroleum ether - acetone - acetic acid 70:30:1. Quantitative Ag-TLC on silica gel impregnated by dipping into a 0.5 % methanolic silver nitrate solution - also preparative Ag-TLC. Quantitative TLC on RP by densitometry at 450 nm. Ag-TLC provided the quantitative data for the TAG classes differing in unsaturation, and RP-TLC for the TAG species differing in chain length within a given class.

J. Lipid Res. 38, 1482-1488 (1997). TLC of phospholipids and neutral lipids on EDTA-impregnated silica gel and after pre-concentration with chloroform - methanol - water 60:40:10 with five step-wise developments: i) chloroform - methanol - water 65:40:5 to 2 cm, ii) ethyl acetate - 2-propanol - ethanol - chloroform - methanol - 0.25% KCl 35:5:20:22:15:9 to 5 cm, iii) toluene -diethyl ether - ethanol 60:40:3 to 7.5 cm, iv) n-heptane - diethyl ether 94:8 to 10.5 cm, v) pure n-heptane to 12.5 cm. Charring by dipping in a solution of 10% cupric sulfate in 8% phosphoric acid for 10 s and heating at 200°C for 2 min. Quantitative determination with an image analyzer in transmission mode.