Nature - Sci. Rep. 11, 62 (2021). Samples were isopropylacetate – methanol 3:2 fractions of (1) n-hexane extracts of larvae of Apis mellifica carnica (Apidae) from hives exposed to different concentrations of neonicotinoid pesticide clothianidin in their food, as well as (2) worker jelly adsorbed from brood combs of the same hives on adsorptive filter strips (unused filter strip parts were kept as background control). HPTLC on silica gel with chloroform – methanol – water – ammonia 30:17:2:1 for (1), and with an 8-step gradient based on methanol, chloroform, toluene, and n-hexane for (2, see CBS 105: Optimization of an AMD 2 method for determination of stratum corneum lipids). Visualization at UV 366 nm before and after derivatization by immersion into primuline reagent (primuline 0.5 g/L in acetone – water 4:1). Furthermore, antibacterial activity of (2) was assessed by recording the bioluminescence on the HPTLC plates, neutralized after elution and immersed into Aliivibrio fischeri suspension. Semi-quantitative comparison showed that a higher exposure to clothianidin was correlated with a decrease in lipid composition as well as in antibacterial activity.

J. Planar Chromatogr. 34, 411-418 (2021). HPTLC of sphingomyelin in erythrocyte membranes of patients on silica gel with chloroform - methanol - acetic acid - water 60:50:1:4. Detection by exposure to iodine vapor for 30 min. Quantitative determination by densitometric measurement of the intensity of individual zones and the red, green and blue values of the component colors. The hRF value for sphingomyelin was 86. Linearity was between 0.25 and 10 μg/zone. LOD and LOQ were 140 and 410 ng/zone, respectively. Intermediate precisions were below 2 % (n=3). Recovery was between 85 and 97 %.

Rapid Commun. Mass Spectrom. 34, 8875 (2020). HPTLC of glycolipids in sperm from different fish species on silica gel with chloroform - ethanol - water - triethylamine 30:35:7:35. Detection by dipping into primuline (dissolved in acetone - water 4:1). Further analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and electrospray ionization ion trap (ESI-IT)MS.

J. Food Compos. Anal. 99, 103869 (2021). HPTLC of phosphatidylcholine (1), phosphatidylethanolamine (2), phosphatidylserine (3), lysophosphatidylcholine (4), and cardiolipin (5) in edible insects (crickets, migratory locusts, and silkworms) on silica gel with chloroform - methanol - 28 % ammonia 13:7:1. Detection by exposure to iodine. The hRF values for (1) to (5) were 24, 51, 13, 15 and 70, respectively. 

Food Chem. 375, 131824 (2022). HPTLC of triacyclglycerols (1), diglycerides (2), monoglycerides (3) and medium and long chain free fatty acids (4) in an alternative
functional food through bulk compound chocolate on silica gel with hexane - ethyl ether - acetic acid 80:20:1. Detection by spraying with 50 % sulfuric acid, following by heating at 150 °C for 10-15 min. Quantitative determination by absorbance measurement at 500 nm.

Food Chem. 357, 129135 (2021). HPTLC of cinnamon on silica gel with toluene - ethyl acetate - methanol 6:5:3. Nine detection modes were used: 1) white light illumination, 2) UV 366 nm, 3) UV 254 nm, and six different derivatization reagents applied by immersion: 4) primuline reagent (100 mg primuline, 20 mL water and 80 mL acetone), 5) p-anisaldehyde sulfuric acid reagent (1 mL methoxy benzaldehyde, 140 mL methanol, 16 mL acetic acid and 8 mL sulfuric acid), 6) vanillin sulfuric acid reagent (1 g vanillin, 80 mL ethanol and 0.8 mL sulfuric acid), 7) diphenylamine aniline o-phosphoric reagent (2 % each of diphenylamine and aniline in 100 mL isopropanol plus 20 mL o-phosphoric acid), 8) Fast Blue B salt reagent (100 mg Fast Blue B salt in 100 mL ethanol, 70 %) and 9) natural product reagent (1 g 2-aminoethyl diphenyl borate in 100 mL ethanol), followed by heating at 110 °C (5), 120 °C (4, 6) or 140 °C (7, 8) for 3-5 min. Effect-directed profiling was performed through eight different assays: HPTLC–Aliivibrio fischeri bioassay, HPTLC–Bacillus subtilis bioassay, HPTLC–tyrosinase inhibition assay and densitometric evaluation, HPTLC–α–glucosidase and β–glucosidase inhibition assays, HPTLC–AChE and BChE inhibition assays, HPTLC–DPPH assay. Compounds were further characterized by heated electrospray ionization high–resolution mass spectrometry (HESI–HRMS).

Chemosphere. 90, 2157-2171 (2013). HPTLC of glycerophospholipids in liver and brain of male Atlantic cod (Gadus morhua) on silica gel (washed with methyl acetate - isopropanol - chloroform - methanol - 0.25 % KCl 25:25:25:10:9 and activated at 120 ºC for 30 min) with hexane - diethylether - acetic acid 40:10:1. Detection by spraying with 0.1 % dichlorofluorescin in 98 % methanol and 0.001 % 3,5-di-tert-4butylhydroxytoluene (BHT). Lipid classes were futher analyzed by gas chromatography with a flame ionization detector. 

Anal. Bioanal. Chem. 413, 1941-1954 (2021). HPTLC of sulfoquinovosylmonoacylglycerol (1) and sulfoquinovosyldiacylglycerol (2) in a cyanobacterium (Arthrospira sp.), a microalgae preparation (Chlorella vulgaris) and selected leafy vegetables (spinach, basil) on silica gel with chloroform - methanol - acetic acid - water 425:75:50:18. Detection by dipping into  5 % copper sulfate in 8 % phosphoric acid, followed by heating at 160 ºC for 30 min.