PLoS ONE 10(10), e0140782 (2015). HPTLC of lipids, extracted from several cancerous cell lines by an adapted Bligh and Dyer’s method, on silica gel with chloroform – methanol 4:1. Detection under UV after spraying with thioflavine S (10 μg/mL in acetone 80 %), glycosylated lipids were hued in red-rose by spraying with orcinol 0.5 % in sulfuric acid 0.5 M followed by heating; some of those lipids were absent from the elisidepsin-resistant strains. These lipids were purified by scraping off the plate and by extracting with chloroform – methanol 2:1, then they were applied on a nitrocellulose membrane for an overlay assay for binding with an elisidepsin-biotin derivative (dot-blotting). One lipid was positive and was identified by NMR as probably a glucosylceramide.

Food Res. Int. 111, 361-370 (2018). HPTLC of triacylglycerols in unfermented and fermented cocoa beans on silica gel with hexane – diethyl ether – glacial acetic acid 80:20:1 and then with chloroform – methanol – glacial acetic acid 97:3:1. Detection by spraying with cerium molybdate reagent. Direct comparison of fermented and unfermented beans revealed the absence of cacaoic acid at hRf 55._x000D_

Lipids 17, 469-475 (1982). The total methyl esters of the fatty acids of tissue glycerides were converted into methoxy-acetoxy-mercury adducts. The adducts were fractionated into groups of equal numbers of ethylenic bands on silica with hexane - dioxane 6:4. Separation of the monoethylenic acids of equal chain length into the respective cis-and trans isomers by TLC on AgNO3-silica, acc. to known procedure.

of Jp. Biochem. Soc. (Saikagaku) 54, 133-135 (1985). (Japanese) (Sensitive enzyme-immunostaining and densitometric determination on thin-layer chromatography of glycosphingolipids by using affinity-purified antiglycosphingolipid antibodies.).

Improvement and evaluation of a method using molybdenum blue reagent for detection. J. Chromatogr. 375, 255-266 (1986). TLC of phospholipids on silica with chloroform - methanol - water 65:25:4, and chloroform - acetone - methanol - acetic acid - water 50:20:15:10:5. Detection by spraying, with molybdenum blue reagent. Quantification by densitometry at 700 nm. Derivatization also by spraying with 3 % copper acetate solution in 8 % aqueous phosphoric acid and heating at 140 °C for 20 min. Densitometry at 450 nm.

Quantification of the phosphorus content of each fraction by colorimetry after reaction with perchloric acid. 5 different developing conditions were used with soybean lecithin and egg yolk lecithin etc. The repeatability and the reproducibility of the determination were acceptable for the lecithins.

J. Chromatogr. 424, 445-448 (1988). TLC of phosphoinositides on silica with chloroform - methanol - 5m MEDTA containing 3.3M NH3 100:80:25 after being labelled with 32PO4 and extracted with chloroform - methanol - hydrochloric acid 20:40:1 containing Janus Green. Measurement by autoradiography.

J. Chromatogr. 454, 425-431 (1988). Two dimensional TLC on plates, containing two parts formed by derivatization of part of the silica layer with ODS and impregnating the remainder with silver nitrate. Development with chloroform - methanol 95:5 followed by chloroform - methanol 98.75:1.25 for argentation TLC and twice with acetonitrile - tetrahydrofuran 4:1 for reversed-phase TLC. Detection by autoradiography.