Budapest Chromatography Symposium, June 13, 1985. Separation of serum neutral lipids and cholesterol ester on silica with toluene - CCl4 1:3 and toluene. Separation of phospholipids with chloroform - methanol - acetic acid - acetone - water 30:9:4:2.5:2.

J. Chromatogr. 342, 385-392 (1985). TLC of neutral glycolipids and peracetylated neutral glycolipids on silica with a) chloroform - methanol - water 65:24:4, b) dichloromethane - methanol 98:2, c) chloroform - methanol water - NH3 (28 %) 40:10:0.9:0.15. Comparison of the results obtained with or without peracetylation, on silica with or without borate impregnation.

Acta Alimentaria 16, 111-127 (1987). TLC of extracted lipids on silica with petrol ether - diethyl ether - acetic acid 80:20:2. Detection after spraying with either saturated SbCl3 solution in chloroform or 1 % solution of vanillin in alcohol containing 10 % sulfuric acid, and heating the plates to maximum color intensity at about 70 °C.

Pharmazie 42, 341-344 (1987). TLC of phospholipids on 1% potassium oxalate-impregnated silica with chloroform - acetone - methanol - acetic acid - water 40:15:13:12:8. Location by autoradiography or by iodine staining. Quantification by liquid scintillation after elution.

Einfache dünnschicht-chromatographische Identifizierung von fetten Ölen, Wachsen und häufig verwendeten Salbengrundlagen. (TLC in the pharmacy. Simple TLC identification of fatty oils, waxes and often used base compounds of ointments.) Dtsch. Apoth. Ztg. 128, 1689-1693 (1988). TLC of fatty oils (extract of linseed and oils of avocado, peanuts, cacao butter, laurel, maize, almonds, olives, castor, safflor, sesame, sunflower) on paraffin- impregnated cellulose with acetic acid. Detection with iodine and spraying with 1% starch solution. TLC of waxes and other base compounds of ointments (e.g. white wax, cetaceum, artificial cetaceum, cetyl palmitate, glycerol monostearate, cetyl stearyl alcohol etc.) on silica with n-hexane - ether - acetic acid 90:10:1. Detection with 10% ethanolic molybdatophosphoric acid, heating for 5-10 min to 150°C, improvement of color development by treatment with NH3 chamber; 0.1% aq. solution of ammonium 8-anilino-naphthalene-1-sulfonate.

J. Chromatogr. 454, 432-437 (1988). TLC on sintered silica with 1.) ethyl acetate - propanol - chloroform - methanol - water - acetic acid 25:25:25:10:6:0.5, 2.) chloroform - methanol - water - acetic acid 45:15:2:0.2, 3.) chloroform - methanol - water - 28% NH3 45:15:2:0.1. Detection by spraying with a) 5 mL of 1% aqueous ammonium molybdate + 5 mL of 25% HCl + 90 mL acetone, b) the mixture of 2 g Brilliant Green + 350 mL water and 4 g ammonium molybdate + 40 mL water + 10M HCl. Determination by densitometry at 620 nm.

J. Planar Chromatogr. 2, 158-160 (1989). TLC of lypophilic fraction of hydrobionts on different commercial plates with various physical characteristics. For hydrocarbons (hexadecylbenzene, dimethyl naphthalene, phenanthrene and 3,4 a-benzopyrene) the selectivity does not change with the grain diameter of the sorbent (5 - 25 µm). The Rf-value is markedly influenced by chamber saturation and specific surface areas. Plates containing a silicium acid sol as binder may be used 10 - 12 times after regeneration (increase of reproducibility).

Optimization of mobile phase, detection and direct evaluation. J. Chromatogr. 502, 385-392 (1990). TLC of phospholipids on silica with a mixture of chloroform – methanol – water. Optimization of the mobile phase by using a mobile solvent composition triangle. Improvement of separation of phospholipids by using acetate buffer (pH 4) in the mobile phase instead of water and by impregnation of the silica layer with phosphoric acid. Detection by spraying with Dittmer-Lester reagent. Quantification by densitometry at 720 nm.