J. Planar Chromatogr. 12, 196-201 (1999). HPTLC of sterols, triacylglycerols, phosphatidylcholine, phosphatidylethanolamine on silica gel; neutral lipids with petroleum ether - ether - acetic acid 80:20:1 in a solvent-equilibrated chamber. Detection by spraying with 5% phosphomolybdic acid in ethanol and heating at 110°C for 10 min. Quantitation by densitometry at 700 nm. Separation of phospholipids with chloroform - methanol - water 65:25:4, detection by spraying with 10% cupric sulfate in 8% phosphoric acid with heating for 20 min; quantitation by densitometry at 370 nm. Qualitative determination of monoacylglycerols and diacylglycerols with a dual-solvent system (acc. to Sipski): Development to a distance of 7 cm with isopropyl ether - acetic acid 24:1, drying with a hair dryer, and development again in the same direction for a distance of 9 cm with petroleum ether - ether - acetic acid 90:10:1.

JAOAC 84, 1277-1282 (2001). HPTLC of 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG), cholesterol, alkylphosphocholines, and analogues and their related compounds (main degradation products) on silica gel with chloroform (for cholesterol), chloroform - methanol - 25% NH3 (for DPPG-sodium) and chloroform - methanol - sodium acetate 1 mol/l in 25% NH3 7:4:1 for miltefosine and perifosine. For AMD, 2 new gradient systems were developed. Visualization by dipping into a copper (II) sulfate solution (10 g copper sulfate dissolved in 100 ml 8% phosphoric acid) for a few seconds and heating of the plates to 180°C for 20 min. Quantification by densitometry at 500 nm.

J. Planar Chromatogr. 18, 141-146 (2005). TLC of fatty acids (ethanoic, propanoic, butanoic, pentanoic, hexanoic, heptanoic, and octanoic acid) on silica gel with hexane - acetone 4:1 or acetone - water - chloroform - ethanol - aqueous ammonia 30:1:3:5:11 with chamber saturation for 30 min. Only bromocresol green, bromophenol blue, potassium permanganate, and methyl red can be used for the detection. Among the new visualizing agents dipping of the plates in an aqueous solution of alkaline blue enables the detection of the free fatty acids from propanoic to octanoic. Dipping generated better contrast regarding the spots than spraying.

Biochem. Biophys. Res. Commun. 399, 716-720 (2010). HPTLC of globotriaosylceramide (Gb3) in mouse tissues on silica gel with chloroform - methanol - water 65:25:4. Detection by HPTLC-immunostaining with an anti-Gb3 monoclonal antibody. Quantification by densitometry with a luminescent image analyzer. The limit of detection of Gb3 was 50 ng/zone.

Anal. Biochem. 218, 224-226 (1994). Reverse-phase TLC of the products of title compounds incubated with CMP-N-acetylneuraminic acid (5x104 cpm) on RP-18 with water. Quantification by computed radiography involved photostimulated luminescence. The procedure is reliable for the simultaneous and rapid measurement of a large number of samples.

J. Lipid Res. 36, 1848-1855 (1995). HPTLC of glycosphingolipids on silica gel with chloroform - methanol - 0.2% CaCl2 60:35:8 and of gangliosides with chloroform - methanol - 0.2% CaCl2 55:45:8. Visualization of glycosphingolipids by spraying with 0.1% 5-hydroxy-1-tetralone in 80% H2SO4. Heating at 120°C for 10 min. Detection under UV 365 nm. Quantification by fluorodensitometry at 470/>500 nm. Lower limits of detection 2 - 5 pmol. New sensitive fluorescent labeling reagent for glycosphingolipids.

Anal. Biochem. 360 (1), 130-137 (2007). Determination of cholesteryl ester hydroperoxides, especially cholesteryl linoleate hydroperoxide isomers, by combination of TLC blotting with diphenyl-1-pyrenylphosphine fluorescent detection (DPPP-TLC blotting) and GC-electron ionization-MS (GC-EI-MS). After DPPP-TLC blotting of cholesteryl ester hydroperoxides fluorescent zones were obtianed which were extracted and derivatized by hydrogenation, transmethylation, and trimethylsilylation. The resulting methyl ester/trimethylsilylether derivatives of hydroxyoctadecenoic acid were then analyzed by GC-EI-MS using selected ion monitoring of isomer-specific fragment ions originating from the alpha-cleavage of the trimethylsilyloxyl group.

nauk SSSR, serija chimichestkaja 6,1405-1408 (1982) (Russian). (IR and UV spectrum of alpha and beta-methylhydrazides of aromatic carboxylic acids.) TLC of N'N-dimethylhydrazide, p,p'-diphenyloxydicarboxylic acid on silica with chloroform - ethanol 7:1. Detection with ninhydrin.