J. Chromatogr. 363, 462-467 (1986). Conversion of phospholipids to dimethyl esters of phosphatidic acids with the exception of phosphatidylinositol which was converted to the pentaacetyl derivatives. TLC on AgNO3-impregnated silica with mobile phases containing chloroform - methanol. The method was used for the analysis of molecular species of egg lecithin.

H. TRAITLER, A. STUDER, R.E. KAISER (eds): Instrumental HPTLC, Institute for Chromatography, Bad Dürkheim, FRG (1987), 379-387. Anticircular TLC of impurities of Jojoba wax esters and decomposed triglycerides on very thin silica layer with petrol ether - ether 6:4. Detection by dipping in manganese chloride - sulfuric acid reagent and heating at 150 °C for 50 sec. Quantification by densitometry at 546 nm.

J. Chromatogr. 446, 259-266 (1988). Reverse-phase TLC on silanized Kieselguhr G with acetone - acetonitrile - water in different proportions. Densitometry at 450 nm. Investigation of the relationship between partition numbers and the maximum-to-maximum distances of the corresponding peaks. Identification by their partition numbers. Presentation of an example concerning sunflower oil triglycerides.

Detection by spraying with 2',7'-dichlorofluorescein, followed by heating to 100°C for 5 min. and viewing under UV 366 nm. Spray reagent: 50 mg dichlorofluorescein and 100 mg aluminum chloride dissolved in 100 mL methanol.

J. Planar Chromatogr. 2, 19-23 (1989). Separation of phospholipids into classes and individual compounds by HPTLC on silica impregnated with boric acid in ethanol. A new fluoregenic reagent 4-(N,N-dihexadecyl)amino-7-nitrobenz-2-oxa-1,3-diazole (NDB dihexadecylamine) was added to the 6-component (!) developing solvent. Densitometry by fluorescence 366/>420 nm. Determination limit about 300 ng. RSD 3-9%.

J. Planar Chromatogr. 3, 55-60 (1990). A strategy for the optimization of chromatographic conditions in TLC-separation of the lipid classes in lecithins of egg yolk, rapeseed, and soybean origin was developed. This approach utilizes factorial design in sequential blocks to screen the variables (in this work, 15 in number), principal component analysis to map the experimental domain, and partial least squares regression for the final optimization. Chromatography on silica, postchromatographic derivatization with 3% cupric acetate in 8% o-phosphoric acid heated at 180°C for 8 min, substances appear as brownish spots. Scanning by absorbance at 350 nm.

Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 301-309 (1991).HPTLC on silica with chloroform - methanol - 0.025% aqueous CaCl2 60:40:9. Covered with a polyvinylidendifluoride (PVDF) membrane negatively loaded gangliosides were electrotransfered to the PVDF-membrane. On the membrane visualization (with resorcinol reagent) or immunoblotting.

Phytochemistry 31, 1961-1967 (1992). Separation of different lipid classes by two-dimensional TLC on silica with 1) chloroform – methanol – water 65:25:4 and 2) chloroform – acetone – methanol – acetic acid 10:4:2:1. Detection by spraying with 10% primuline in aqueous 80% acetone and under UV.