Thin Layer Chromatographic and densitometric analysis. J. Planar Chromatogr. 20, 209-215 (2007). TLC of phospholipids (with cardiolipin, phosphatidyl ethanolamine plasmologen and phosphatidyl cholin plasmologen as standards) on silica gel, prewashed with chloroform - methanol 2:1 and acetone, using one-dimensional TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 and two-dimensional TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 in the first direction and hexane - diethyl ether 1:1 in the second direction after hydrolysis with 1 % hydrochloric acid to reveal alkenylphospholipids. Detection by staining with thionine reagent resp. with leucofuchsin reagent. Densitometric scanning at 600 nm (for thionine) and at 560 nm (for leucofuchsin).

J. Liq. Chromatogr. Relat. Technol. 33, 1005-1012 (2010). HPTLC of lipids (free sterols, free fatty acids, triacylglycerols, methyl esters, and steryl esters) on silica gel (plates with concentration zone) with petroleum ether - diethylether - glacial acetic acid 80:20:1. Detection by spraying with 5 % ethanolic phosphomolybdic acid reagent and heating for 10 min at 110 °C. Quantitative densitometric analysis was performed at 610 nm.

J. Sep. Sci. 37, 2062-2068 (2014). HPTLC of (1) phospholipids phosphatidylcholine, (2) phosphatidylethanolamine, (3) phosphatidylinositol, (4) phosphatidylserine and (5) sphingomyelin in Phyrrhocoris apterus on silica gel with chloroform - methanol - acetic acid - water 60:35:2:1 over a path of 9 cm, and chloroform - methanol - acetic acid - water 25:15:4:2 over a path of 6 cm. Detection by exposure to iodine vapor, followed by detection at UV 205 nm. The hRF values of (1) to (5) were 91, 68, 82, 88 and 94, respectively. The spots were subsequently scrapped from the plates. Quantification by conversion into inorganic phosphate (as phosphomolybdate complex) followed by absorbance measuremet at 820 nm.

J. Agric. Food Chem. 64, 8838-8847 (2016). HPTLC of free fatty acids produced after treatment of vegetable oils with crude lipase extracts from germinated seeds of Adansonia suarezensis, Adansonia grandidieri, Moringa oleifera, Moringa drouhardii, Jatropha mahafalensis, and Jatropha curcas seeds on silica gel with hexane – diethyl ether – acetic acid 70:30:1. The hRF value of free fatty acid was 43.

J. Agric. Food Chem. 32, 797-800 (1984). Development of a HPTLC screening method of lipoidal and polar component extracts on silica with hexane - ether - acetic acid 90:10:1 for the chloroform extract and benzene - acetic acid - water - nitromethane 6:4:1:3 for the aqueous methanol extract.

Budapest Chromatography Symposium, June 13, 1985. Separation of serum neutral lipids and cholesterol ester on silica with toluene - CCl4 1:3 and toluene. Separation of phospholipids with chloroform - methanol - acetic acid - acetone - water 30:9:4:2.5:2.

J. Chromatogr. 342, 385-392 (1985). TLC of neutral glycolipids and peracetylated neutral glycolipids on silica with a) chloroform - methanol - water 65:24:4, b) dichloromethane - methanol 98:2, c) chloroform - methanol water - NH3 (28 %) 40:10:0.9:0.15. Comparison of the results obtained with or without peracetylation, on silica with or without borate impregnation.

Acta Alimentaria 16, 111-127 (1987). TLC of extracted lipids on silica with petrol ether - diethyl ether - acetic acid 80:20:2. Detection after spraying with either saturated SbCl3 solution in chloroform or 1 % solution of vanillin in alcohol containing 10 % sulfuric acid, and heating the plates to maximum color intensity at about 70 °C.