Chem. Anal. (N.Y.) 108, 157-166 (1990). Description of the method of TLC/FTIR spectrometry employing mass transfer from the TLC plate to an IR transmittant carrier. Demonstration of the advantages of the method using a standard mixture of phospholipids.

J. Planar Chromatogr. 3, 126-132 (1990). HPTLC separation of lipids on silica with hexane - ether 6:4 and after intermediate drying, with chloroform - acetone - methanol 19:1:5 to 20 - 25 mm, then again after drying, with chloroform - acetone - methanol - formic acid 85:10:5:0.6 to 90 mm in a presaturated chamber. After chlorination of the specific NH-groups, quantification by transmittance scanning densitometry. Subsequent detection with a chromogenic benzidine reagent; identification of the CM classes using a standard mixture of spingomyelin derived ceramides. Quantification levels as low as 100 ng per individual class.

J. Planar Chromatogr. 4, 397-401 (1991). TLC of triglycerides (i.a. trilinolein, triolein, dipalmitoolein, palmitostearoolein) on silica impregnated with 0.5% methanolic silver nitrate solution or on RP-2 silica. Eluent for argentation TLC was petrol ether - acetone 20:9. After development the plates were dried for at 110°C, then treated consecutively with bromine vapor and sulfuryl chloride vapor, followed by charring at 180-200°C. Densitometry.

J. Liquid Chromatogr. 16, 1819-1831 (1993). TLC of major phospholipid classes on silica with chloroform - methanol - ethyl acetate 40% methylamine 2:2:2:1. Detection by heating at 180 °C for 3 min., spraying with 10% H3PO4, and charring at 180 °C for 20 min. Quantification by densitometry at 400 nm.

Anal. Biochem. 223, 232-238 (1994). Description of a method for purifying glycosphingolipids and phospholipids by using TLC blotting. Separation of glycosphingolipids by two-dimensional TLC and detection with primuline reagent. Transfer of the separated spots by TLC blotting to a polyvinylidene difluoride membrane, and extraction; monitoring by TLC.

J. Agric. Food Chem. 46, 1836-1843 (1998). Combination of different chromatographic techniques. TLC of triglycerides on silica gel incubated overnight with 20% silver nitrate solution. After drying activation at 100°C for 30 min. The mobile phase for the triglycerides (incl. triolein and tristearin as standards) was chloroform. Visualization by spraying with a 0.15% ethanolic solution of 2',7'-dichlorofluorescein and observation under UV. Quantitation after elution by GC.

J. Planar Chromatogr. 12, 397-399 (1999). HPTLC of neutral lipids (e.g. cholesteryl oleate, methyl oleate, triolein, oleic acid, cholesterol) on silica gel with petroleum ether - ether - acetic acid 40:10:1, after precleaning of the plates with dichloromethane - methanol 2:1. Detection as blue zones against a yellow background by spraying with a 50 % ethanolic phosphomolybdic acid and heating at 110°C for 15 min. Quantitation by densitometry at 700 nm.

J. Liq. Chrom. & Rel. Technol. 24, 1479-1490 (2001). Description of a portable system for performing rapid TLC screening of tubercolosis pharmaceuticals (ethambutol hydrochloride, isoniazid, pyrazinamide, rifampin, streptomycin sulfate and combinations thereof) using a polyethylene bag as the developing chamber with the iodine detection method designed for use in field-type operations. TLC on silica gel coated plastic sheets containing a fluorescent indicator F 254. Mobile phases used are methanol - conc. NH3 25:0.38, methanol - acetone - conc. NH3 13:17:1, or ethyl acetate - acetic acid - conc. NH3 - water 3:3:1:1. Detection under UV 254 nm or by dipping into a iodine - potassium iodide detection reagent solution. Quick, economical, and reliable TLC method.