J. Sliwiok (ed.): Acta Chromatographica 10, 183-189 (2000). HPTLC of triacylglycerols, free sterols, cholesteryl ester, phosphatidylethanolamine, phosphatidylcholine, and free fatty acids in the digestive gland-gonad complex of Cerithidia californica snails infected with Euhaplorchis californiensis, Cloacitrema michiganensis, and Mesostephanus appendiculatus on silica gel with either petroleum ether - diethyl ether - acetic acid 80:20:1 or (esp. for cholesterol esters) n-hexane - petroleum ether - diethyl ether - glacial acetic acid 50:20:5:1 or (esp. for phospholipids) with chloroform - methanol - water 65:25:4. Quantification of neutral lipids at 700 nm after derivatization with phosphomolybdic acid and of phospholipids at 370 nm after derivatization with cupric sulfate reagent.

J. Planar Chromatogr. 15, 177-182 (2002). HPTLC of 12 fatty acids, hydroxy fatty acids and methyl esters (oleic acid, elaidic acid, palmitic acid, stearic acid, ricinoleic acid, methyl ricinoleate, a-hydroxypalmitic acid, methyl a-hydroxypalmitate, 12-hydroxystearic acid, methyl 12-hydroxystearate, 9,10-dihydroxystearic acid, methyl 9,10-dihydroxystearate) on RP-18 with methanol and methanol - water 19:1 and 9:1. previously saturated for 30 min. Visualization by exposure to iodine vapor. The most accurate prediction of the RM values of the acids and esters, for all the mobile phases investigated, was achieved by use of four-parameter equations relating the dipole moments of the mobile phases to three other parameters.

J. Planar Chromatogr. 16, 227-229 (2003). HPTLC of 8 methyl esters of higher fatty acids (methyl laureate, methyl myristate, methyl palmitate, methyl stearate, methyl 12-hydroxystearate, methyl 9,10-dihydroxystearate, methyl ricinoleate, methyl a-hydroxypalmitate) on RP-18 with methanol, ethanol, and mixtures of methanol and ethanol in the volume proportions 19:1, 9:1, 17:3, 4:1, and 3:1. Visualization by exposure to iodine vapor. Relationships between RF values and mobile phase composition were determined for the esters investigated.

J. Liq. Chrom. Rel. Technol. 27, 1967-1980 (2004). Part of a study originally devoted to investigating lateral interactions of monocarboxylic and a,?-dicarboxylic acids analyzed on a low-activity stationary phase (cellulose powder) with low-polarity monocomponent mobile phases (decalin for monocarboxylic acids and 1,4-dioxan for a,?-dicarboxylic acids). TLC of lauric, myristic, palmitic, 3-phenylpropionic, 2-phenylbutyric, and 4-phenylbutyric acid with decalin and of succinic, adipic, and suberic acid with 1,4-dioxan on cellulose. Detection with an ethanolic solution of bromocresol green. Quantitative determination at 625 nm.

J. Liq. Chromatogr. & Relat. Technol. 29, 2787-2794 (2006). HPTLC of acids (from octanoic to octadecanoic) on RP-18 with methanol - water 9:1 and ethanol - water 19:1. Detection with iodine vapor. The characterisation by high values of determination coefficients suggest the possibility of using them to calculate and predict the values of solubilities in water of acids investigated.

J. Chromatogr. 394, 353-362 (1987). Purification of prostaglandins El, E2 and E3 by 1-D and 2-D TLC on silica with diethyl ether - methanol for the former, and diethyl ether - acetic acid 100:2 and chloroform - methanol - acetic acid 90:10:2 for the latter. Quantification by HPLC. Verification by MS after preparative TLC on silica with diethyl ether - methanol 98:2.

Anal. Bioanal. Chem. 392, 849-860 (2008). HPTLC of cell lipids on silica gel with chloroform - ethanol - water - triethylamine 5:5:1:5. Detection under UV 366 nm after spraying with primuline (Direct Yellow) reagent. Coupling with MALDI-TOF-MS which is traditionally used for proteomics, but is also a useful tool for lipid analysis. Depending on the applied matrix, however, some lipid classes are more sensitively detected than others and this may even lead to suppression effects if complex mixtures are analyzed. Therefore, a previous separation into the individual lipid classes is necessary. Using artificial lipid mixtures or easily available tissue extracts, it has already been shown that lipids can be conveniently analyzed by MALDI-TOF MS directly on the TLC plate. An initial TLC-MALDI-TOF-MS study of the lipid composition of ovine mesenchymal stem cells is presented. Due to the complex composition of these cells, data are also compared to lipids extracted from human erythrocytes. Even very minor lipid classes can be easily detected and with much higher sensitivity than by common staining protocols.

Trends in Chromatography 8, 1-6 (2013). HPTLC of neutral lipids and a neutral lipid standard (consisting of 20 % each of cholesterol, oleic acid, triolein, methyl oleate, and cholesteryl oleate) on silica gel (HLF plates with 19 scored channels of 9 mm width) prewashed with dichloromethane - methanol 1:1 and heated for 30 min at 120 °C, with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 with chamber saturation. Detection by spraying with 5 % ethanolic phosphomolybdic acid reagent and heating at 120 °C for 5 min. Neutral lipids appeared as blue zones on a yellow background. The fractions of free sterols, free fatty acids, triacylglycerols, methyl esters, and steryl esters were identified by comparison with the standard mixture. Quantification by absorption measurement at 610 nm via linear calibration (peak area).