J. Liq. Chrom. & Rel. Technol. 25, 1615-1621 (2002). HPTLC of lipids, mainly free fatty acids and free sterols, on silica gel with petroleum ether - diethyl ether - acetic acid 80:20:1 and detection with phosphomolybdic acid. Separation of phospholipids, mainly phosphatidylcholine and phosphatidylethanolamine, on silica gel with a mobile phase of chloroform - methanol - water 65:25:4. Detection by spraying with a 10% cupric sulfate in 8% phosphoric acid and heating at 140°C for 15 min. Quantitation by densitometry at 370 nm. Reexamination of a 35 year-old publication.

Mini review and chromatographic-computional analysis. Acta Chrom. 17, 9-19 (2006). Comparative correlative TLC analysis conducted on whole-tissue homogenate of control and in-vitro-incubated samples of mammalian myocardia and bullfrog cardiac muscle and thigh skeletal muscle. The focus in the study was on TLC-densitometric analysis of the endogenous cardiolipin of control samples and their respective incubated samples to identify the products, e. g. lysocardiolipin, formed during in-vitro incubation. TLC of the chloroform-methanol extracts (2:1) obtained after the incubation from the freeze-dried tissues homogenate on silica gel with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18. Visualization with thionine reagent, densitometry at 600 nm.

Microbiol. Res. 166, 255-267 (2011). TLC of lipopeptides (produced by Pseudomonas species in cultures of Pleurotus ostreatus; Pseudomonas reactans was used as a reference) on silica gel with chloroform – methanol – ammonia 80:25:4. Detection by spraying with 0.1 % bromothymol blue in ethanol, followed by heating.

Rapid Commun. Mass Spectrom. 29, 1288-1296 (2015). HPTLC of sphingomyelins (1), phosphatidylcholines (2) and phosphatidylethanolamines (3) on silica gel with chloroform - methanol - water - acetic acid 30:15:2:4 for the first step and acetone - acetonitrile - chloroform 5:4:2 for the second step. Detection by spraying with 0.5 % amido black 10B stain in 1 M NaCl. The method was coupled with matrix-assisted laser desorption/ionisation mass spectrometry for the analysis of (1) and (2) extracted from 5,6-dimethylxanthenone-4-acetic acid-treated xenograft tumours.

Anal. Biochem. 225, 24-27 (1995). Glycosphingolipids separated by TLC on silica with chloroform - methanol - 0.2% CaCl2 60:35:8 were transferred to a polyvinylidene difluoride membrane by TLC blotting. Analysis by SIMS. Detection limit about 1 µg. The major advantage of this method is that glycosphingolipids can be analyzed structurally without purification by repeated column chromatography.

Anal. Chem. 75, 118-125 (2003). OPLC of acidic glycolipids (e.g. GM3, GD3, GT1b, chondroitin sulfate disaccharides, heparan sulfate tetrasaccharide) on silica gel. Under optimized conditions, a sensitivity of 5 pmol of glycosphingolipid was demonstrated for TLC/ESI-MS and 20 pmol for TLC/ESI-MS/M production scanning to derive the saccharide sequence and long chain base/fatty acid composition of the ceramide. Initial preconditioning of TLC plates is necessary to achieve high sensitivity detection by reducing chemical background noise. Plates can be used repeatedly (at least 10 times) for analysis. Conventional HPTLC on silica gel with chloroform - methanol - water 60:35:8. Detection by nondestructive staining or UV absorption or fluorescence.

J. of Chromatogr. A 1218 (19), 2668-2675 (2011). Use of the changes in emission of berberine cation, induced by non-covalent interactions with lipids on silica gel for detection and quantification of lipids using fluorescence densitometry in HPTLC/AMD. Three different HPTLC/AMD gradients were developed for the separation of 1) neutral lipid families and steryl glycosides, 2) different sphingolipids, and 3) sphingosine–sphinganine mixtures. Rationalization of fluorescent molar responses of studied lipids, and differences in response among different lipid families in the light of a previously proposed model of FDIC response, which is based on ion-induced dipole interactions between the fluorophore and the analyte, likewise, application of computational calculations using molecular mechanics as a complementary useful tool to explain high FDIC responses of cholesteryl and steryl-derivatives, and moderate responses of sphingolipids. Proposal of an explanation for the high FDIC response of cholesterol, whose limit of detection is 5 ng.

Neurochem. Intern. 4, 397-403(1982). Two-dimensional HPTLC of 20 gangliosides (9 identified) on silica with 1) chloroform - methanol - 0.2 % CaCl2 solution 50:40:10, 2) propanol - 17M NH4OH-water 6:2:1. Detection by spraying with p-dimethylaminobenzaldehyde reagent and heating at 120 °C for 15 minutes. Densitometry.