II. Multidevelopment technique. J. Planar Chromatogr. 4, 91-92 (1991). HPTLC of 21 different lipid components on silica with the following mobile phases: 1) propanol – methyl acetate – methanol – ethanol – 43 mM KCl 23:23:29:10:12; 2) hexane – acetone – toluene – chloroform 300:54:25:25; 3) tetrachloromethane. Detection with phosphomolybdic acid reagent (for polar lipids), and CuSO4 (for neutral lipid compounds, glycerides and fatty acids). 1. Publication: L. KOVACS, J. PICK, J. PUCSIK, J. Planar Chromatogr. 2, 389-391 (1989).

Biomed. Chromatogr. 10, 233-236 (1996). TLC on silica with butanol - ethanol - water 4:3:3. Detection by spraying with Dragendorff's reagent. Discussion of the TLC behavior of the compounds.

Anal. Chem. 72, 1759-1766 (2000). TLC of n-alkanes and of components with partially aliphatic structure (e.g. fatty acids, cholesterol, prostaglandins, ceramides, geraniol, and propylene glycol) on berberine-impregnated silica with n-hexane. Densitometry in the fluorescence mode at 356 nm. Proposal of an explanation for the alkane-mediated enhancement of berberin fluorescence: The alkanes create an apolar microenvironment for the excited berberine cation, which lowers the dielectric constant around berberine and hinders alternative relaxation mechanisms, thus favoring fluorescence emission. This interaction of the berberine cation is much stronger than in the case of uncharged p-acceptor molecules like caffeine and tetranitrofluorenone. Analytes with a partially aliphatic structure give also an increase in fluorescence signal.

J. AOAC Int. 91, 1191-1195 (2008). HPTLC of ketorolac tromethamine ((+/-)-5-(benzoyl)-2,3-dihydro-1N-pyrrolizine-1-carboxylic acid tris hydroxymethylaminomethane) on silica gel prewashed with methanol with n-butanol - chloroform - acetic acid - ammonium hydroxide - water 9:3:5:1:2 in a horizontal developing chamber. Quantitative determination by absorbance measurement at 323 nm.

Abstract No. 9226, IHCB (2009). HPTLC of oleanolic acid (in extracts of Ocimum sanctum collected from different geographical sources) on silica gel with toluene - ethyl acetate - glacial acetic acid 55:45:2. Detection by spraying with Liebermann-Burchard’s reagent. Quantitative determination by absorbance measurement at 600 nm. Linearity was in the range of 1-4 µg, recovery was 100.4-102.1 %. Hydro alcoholic extracts of the plant contained high amounts of oleanolic acid, maximum contents were found in plants collected in the Kerala region.

International Journal of ChemTech Research 2(1), 89-96 (2010). A micellar thin layer chromatographic method has been reported for identification of ketoprofen in drug formulation and spiked urine samples, followed by quantification of the drug by HPLC both in formulation and spiked urine samples. TLC on amino layer with a micellar mobile phase of 0.5 % aqueous solution of sodium dodecyl sulphate -Triton X100 - acetone 16:10:3. The drug was extracted from spiked urine samples with ethylene dichloride containing 10 % each of isomyl alcohol and diethyl ether, then the organic phase was evaporated and the residue was taken up in acetonitrile. Derivatization by exposure to iodine vapor and by spraying with Dragendorffs reagent. The hRf value of ketoprofen was 50.

J. Chromatogr. Sci. 48(10), 825-829 (2010). TLC of ibuprofen and its impurities in pharmaceutical preparations on silica gel with toluene - ethyl acetate - glacial acetic acid 17:13:1. Quantification by densitometry. The limit of detection and quantification ranges from 0.13-0.27 µg/zone. The recovery is 96.8-99.0 % for the individual constituents. The method is suitable for routine quality-control analysis of pharmaceuticals containing ibuprofen.

62nd Indian Pharmaceutical Congress Abstract No. F-246 (2010). TLC of atorvastatin calcium, ezetimibe and fenofibrate on silica gel with toluene – methanol – chloroform 6:3:4. Quantitative determination by absorbance measurement at 280 nm. The method was found to be linear in the range of 100-600 ng/band for atorvastatin calcium and ezetimibe and 50-300 ng/band for fenofibrate.