J. Planar Chromatogr. 24, 206-210 (2011). HPTLC of free sterols, free fatty acids, triacylglycerols, methyl esters, and steryl esters in larvae samples on silica gel with concentration zone, with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 in a twin-trough chamber saturated for 20 min at 21 +/- 1 °C and a relative humidity of 25 %. Detection by spraying with 5 % ethanolic phosphomolybdic acid solution. Quantitative determination by densitometry at 610 nm.

PLoS ONE 9(1), e1003109 (2013). In order to understand how Borrelia burgdorferi acquires cholesterol from its host, five experiments were performed. HPTLC on silica gel with chloroform – methanol 17:3, the lipids were extracted through the Bligh and Dyer method: (1) from the spirochete incubated 4 h in a medium containing 4 mg/L BODIPY-cholesterol (fluorescing only in hydrophobic environment), (2) from the spirochete incubated 2 h in a medium containing 10 µCi/L tritiated cholesterol, (3) from HeLa cells incubated with the spirochete itself charged with tritiated cholesterol (after removal of all spirochetes). The same analysis was applied (4) to the purified outer membrane vesicles of the spirochete previously charged with BODIPY-cholesterol. In cases (1) and (4), UV detection showed that the BODIPY-cholesterol is incorporated into the cholesterol glycolipids of B. burgdorferi, whereas without incubation it has the same hRf as cholesterol. In cases (1), (2) and (4), iodine staining made free cholesterol and cholesterol-glycolipids (galactopyranosides) visible, but no other (cholesterol-free) lipids. In case (2), the chromatogram was sprayed with EN3HANCE Spray (DuPont), exposed using BioMax MR Film (Kodak) for 4 and 14 days at −80 °C and developed using a Medical Film Processor Model SRX-101A (Konica); the radiolabelled cholesterol was shown incorporated into the cholesterol-glycolipids. In cases (2) and (3), the radiolabelled zones on the layer were scraped off and analyzed by liquid scintillation counting; cholesterol-glycolipids were found (more than free cholesterol) transferred from the spirochete to the HeLa plasmalemma.

J. Chromatogr. Sci. 56 (1), 92-98 (2018). TLC for direct enantiomeric resolution of the racemic β-adrenolytics bisoprolol (1), atenolol (2), propranolol (3), salbutamol (4) and carvedilol (5) by TLC using bovine serum albumin (BSA) as chiral additive in the stationary phase and with different compositions of simple organic solvents without buffer or inorganic ions. Systematic investigation of the effect of variation in pH, temperature, amount of BSA as the additive, and composition of mobile phase on resolution. Detection by exposure to iodine vapors. The five entantiomers were separated in the elution order (1), (2), (3), (4), and (5). The LOD was 0.7, 1.2, 0.8, 1.6 and 0.9 μg/zone for (1), (2), (3), (4), and (5), respectively.

Lipids 17, 107-110 (1982). TLC of glycolipids (glucocerebromide, lactosyl ceramide, globotriosyl ceramide, gangliotriosyl ceramide, globoside I, asialo-GM1) on silica with chloroform methanol - 0.2 % aq. CaCl2 55:45:10. Detection with anthrone-sulfuric acid reagent.

J. Chromatogr. 323, 462-464 (1985). TLC of radioactive phosphoinositides on silica with chloroform - methanol 3.3 N NH3 75:60:19. The chromatogram is first sprayed with water, then scrolled off with a razor blade and quantificated by scintillation.

Anal. Biochem. 150, 111-116 (1985). HPTLC of non-polar lipids, glycosphingo-lipids on silica with 3 or 4 consecutive developing solvent systems. Separation of more than 20 lipid subclasses with good reproducibility.

J. Chromatogr. 413, 257-263 (1967). Circular TLC of phospholipids on silica with 1) chloroform - methanol acetic acid - water 70:30:4:3, 2) chloroform - methanol - ethanol - acetic acid - water 60:30:10:4:3. Detection by adding 2,5-bis-(5-tert.-butyl-benzoxazolyl(2'))thiophene to the solvent system, and visualizing in UV at 366 nm. Quantification by fluorodensitometry at 366 nm. Detection limit was in ng level; C.V. 0.3-3.1 %.

Anal. Biochem. 168, 115-120 (1988). Separation of glycolipids and gangliosides by TLC. Analysis by fast-atom-bombardment MS after elution, permethylation and purification. Sensitivity, 1-5 µg.