J. Sliwiok (ed.): Acta Chromatographica 10, 183-189 (2000). HPTLC of triacylglycerols, free sterols, cholesteryl ester, phosphatidylethanolamine, phosphatidylcholine, and free fatty acids in the digestive gland-gonad complex of Cerithidia californica snails infected with Euhaplorchis californiensis, Cloacitrema michiganensis, and Mesostephanus appendiculatus on silica gel with either petroleum ether - diethyl ether - acetic acid 80:20:1 or (esp. for cholesterol esters) n-hexane - petroleum ether - diethyl ether - glacial acetic acid 50:20:5:1 or (esp. for phospholipids) with chloroform - methanol - water 65:25:4. Quantification of neutral lipids at 700 nm after derivatization with phosphomolybdic acid and of phospholipids at 370 nm after derivatization with cupric sulfate reagent.

An in vitro study. J. Planar Chromatogr. 15, 396-403 (2002). One- and two-dimensional TLC of cardiolipin, monolysocardiolipin, phosphatidyl ethanolamine, and phosphatidyl choline on silica gel, sequentially washed with chloroform - methanol 2:1 and acetone, in a twin-trough chamber after saturation for a minimum of 1 h. 2D-TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 or 0.05 mL of 14 m NH3 added to 20 mL of the a.m. mobile phase in the first direction and hexane - ether 4:1 in the second dimension, after a hydrolysis step (1% HCl), followed by reaction with Schiff leucofuchsin reagent. Differential staining of the different phospholipids in the one-dimensional chromatogram was performed as described by F. M. Helmy and M. H. Hack, J. Chromatogr. 374, (1986) 61-72. Densitometry at 600 or 560 nm.

J. Liq. Chromatogr. Relat. Technol. 28, 2597-2606 (2005). HPTLC of free sterol, and free fatty acids (cholesterol, triacylglycerol and methyl esters) on silica gel (prewashed with dichloromethane - methanol 1:1) with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 in a twin-trough chamber saturated for 15 min. Determination of steryl esters with n-hexane - petroleum ether - diethyl ether - glacial acetic acid 50:25:5:1. Detection by spraying with 5 % ethanolic phosphomolybdic acid solution and heating for 10 min at 115 °C. Determination of polar lipids (cholesterol, phosphatidyl ethanolamine, phosphatidylcholine, lysophosphatidylcholine) with chloroform - methanol - water 65:25:4. Detection by spraying with a 10 % cupric sulfate solution and heating at 140 °C for 10 min. Quantitation by densitometry at 610 nm (for neutral lipids) and 370 nm (for polar lipids).

Pharm. Res. 33, 1587-1601 (2016). HPTLC study of the cleavability of the disulfide bond in a conjugate siRNA-S-S-PE (incubated in 10 mM glutathione at 37 °C for 4 h) on silica gel with chloroform – methanol 4:1. Detection by spraying with molybdenum blue dye for the cleaved phospholipids. This test confirmed the potential utility of this system for siRNA delivery in vivo.

J. Food Comp. Anal. 44, 45-55 (2015). HPTLC of gangliosides based on the degree of sialylation in the milk fat globule membrane of whole milk on silica gel with chloroform - methanol - 28 % ammonia - water 60:35:7:3. Bands containing gangliosides were excised and glangliosides extracted for LC-MS analysis.

Fat Sci. Technol. 97, 453-454 (1995). Analytical TLC of fatty acids (i. a. cyclopropenoic acid, vernolic acid) on silica with ether - hexane 2:8; 3:7. In addition preparative TLC of mixed fatty acids for separation into oxygenated and nonoxygenated fractions. Detection by spraying with dichlorofluorescein and visualization under UV.

Anal. Chem 75, 6728-6731 (2003). Fluorometric method for the determination of quantities of gangliosides ranging from pico- to nanomoles. HPTLC of sugars (D-(+)-glucose, D(+)-galactose, D(+)-frucose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-neuramic acid) and gangliosides (GM1, asialoGM1, GD1a, GT1b) on silica gel, prewashed with methanol - chloroform 1:1, with chloroform - methanol - 0.2% CaCl2 (aq) 60:35:8 as solvent system in a well-saturated TLC chamber. Detection by spraying with 18% hydrochloric acid thoroughly drying at 40°C in an drying oven and heating for 12 min at various temperatures (from 50 to 180°C). The fluorescence (UV 365 nm) of each sample was greatly dependent on the heating temperature. Calibration curves for gangliosides were obtained by HPTLC and an image-analyzing system equipped with a CCD camera and they showed a high linearity in a wide range from 47 pmol to 4.5 nmol. New and simple procedure.

Parasitol. Res. 107, 947-953 (2010). HPTLC of neutral lipids on silica gel (prewashed by development with dichloromethane - methanol 1:1 and dried for 30 min at 120 °C) with petroleum ether – diethyl ether – glacial acetic acid 80:20:1, detection by spraying with 5 % ethanolic phosphomolybdic acid reagent and heating at 115 °C for 10 min. HPTLC of polar lipids (phosphatidylcholine, phosphatidylethanolamine, sphingomyelin) with chloroform – methanol – deionized water 65:25:4, detection by spraying with 10 % cupric sulfate in 8 % phosphoric acid and heating at 140 °C for 30 min.