Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
- Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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Anal. Chim. Acta 488, 203-209 (2003). Dansylation of aromatic carboxyl compounds (i.e. aspirin), aromatic primary amines, and aliphatic carboxyl compounds in 1 M Na2CO3 buffer at pH 11. TLC on silica gel of fluorescent labeled analytes using methylene chloride, ethyl acetate, acetone, or desired mixture of the solvents. Methylene chloride was superior to ethyl acetate or acetone. Fluorescent analytes were observed under UV lamp. Limits of detection for dansylated carboxyl compounds was 1-5 µg.
Part IV. Separation on RP 18 plates with ternary mobile phases. J. Planar Chromatogr. 18, 228-233 (2005). HPTLC of heptanoic to eicosanoic acids on RP-18 (with and without concentrating zone). The best chromatographic conditions for separation of the fatty acids were RP-18 plates without concentrating zone and methanol - ethanol - water 9:9:2, and RP 18 plates with concentrating zone and methanol - ethanol - water or methanol -n-propanol - water 9:9:2. Detection by exposure to iodine vapour. Separation of acids from methanoic to butanoic and from tetracosanoic to triacontanoic acid was not possible.
Thin Layer Chromatographic and densitometric analysis. J. Planar Chromatogr. 20, 209-215 (2007). TLC of phospholipids (with cardiolipin, phosphatidyl ethanolamine plasmologen and phosphatidyl cholin plasmologen as standards) on silica gel, prewashed with chloroform - methanol 2:1 and acetone, using one-dimensional TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 and two-dimensional TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 in the first direction and hexane - diethyl ether 1:1 in the second direction after hydrolysis with 1 % hydrochloric acid to reveal alkenylphospholipids. Detection by staining with thionine reagent resp. with leucofuchsin reagent. Densitometric scanning at 600 nm (for thionine) and at 560 nm (for leucofuchsin).
J. Liq. Chromatogr. Relat. Technol. 33, 1005-1012 (2010). HPTLC of lipids (free sterols, free fatty acids, triacylglycerols, methyl esters, and steryl esters) on silica gel (plates with concentration zone) with petroleum ether - diethylether - glacial acetic acid 80:20:1. Detection by spraying with 5 % ethanolic phosphomolybdic acid reagent and heating for 10 min at 110 °C. Quantitative densitometric analysis was performed at 610 nm.
separation techniques for the identification and quantification of phospholipids in
complex biological matrices
J. Sep. Sci. 37, 2062-2068 (2014). HPTLC of (1) phospholipids phosphatidylcholine, (2) phosphatidylethanolamine, (3) phosphatidylinositol, (4) phosphatidylserine and (5) sphingomyelin in Phyrrhocoris apterus on silica gel with chloroform - methanol - acetic acid - water 60:35:2:1 over a path of 9 cm, and chloroform - methanol - acetic acid - water 25:15:4:2 over a path of 6 cm. Detection by exposure to iodine vapor, followed by detection at UV 205 nm. The hRF values of (1) to (5) were 91, 68, 82, 88 and 94, respectively. The spots were subsequently scrapped from the plates. Quantification by conversion into inorganic phosphate (as phosphomolybdate complex) followed by absorbance measuremet at 820 nm.
J. Agric. Food Chem. 64, 8838-8847 (2016). HPTLC of free fatty acids produced after treatment of vegetable oils with crude lipase extracts from germinated seeds of Adansonia suarezensis, Adansonia grandidieri, Moringa oleifera, Moringa drouhardii, Jatropha mahafalensis, and Jatropha curcas seeds on silica gel with hexane – diethyl ether – acetic acid 70:30:1. The hRF value of free fatty acid was 43.
J. Agric. Food Chem. 32, 797-800 (1984). Development of a HPTLC screening method of lipoidal and polar component extracts on silica with hexane - ether - acetic acid 90:10:1 for the chloroform extract and benzene - acetic acid - water - nitromethane 6:4:1:3 for the aqueous methanol extract.
Budapest Chromatography Symposium, June 13, 1985. Separation of serum neutral lipids and cholesterol ester on silica with toluene - CCl4 1:3 and toluene. Separation of phospholipids with chloroform - methanol - acetic acid - acetone - water 30:9:4:2.5:2.