I. Separation of human brain lipids. J. Liqu. Chromatogr. 25, 1255-1269 (2002). HPTLC of lipids (glycerophospholipids, sphingomyelin, glycosphingolipids, cholesterol) on silica gel with chloroform - methanol - 0.2% aqueous calcium chloride solution 15:8:1; 90:12:1. Detection by spraying with orcinol reagent and Dittmer's reagent.

J. Liq. Chrom. Rel. Technol. 27, 2039-2045 (2004). HPTLC of cholesteryl oleate, methyl oleate, triolein, oleic acid, and cholesterol on pre-washed silica gel with petroleum ether - diethyl ether - glacial acetic acid 80:20:1 in a twin-trough chamber with saturation. Detection with phosphomolybdic acid reagent. Quantitative determination at 610 nm.

Anal. Chem. 76, 6484-6491 (2004). TLC on silica gel with chloroform - methanol - 0.2 % calcium chloride 11:9:2 or chloroform - 2-propanol - 50 mM potassium chloride 10:67:23 for separation of glycolipids, oligosaccharides, lipids, and compounds of environmental and pharmaceutical interest with coupling to an external ion source MALDI-Fourier transform (FT) MS instrument without compromising mass accuracy and resolution of the spectra. Furthermore, when the FTMS, with >70 000 resolving power, has a vibrationally cooled MALDI ion source, fragile glycolipids can be desorbed from TLC plates without fragmentation, even to the point that desorption of intact molecules from “hot” matrixes such as a-cyano-4-hydroxycinnamic acid is possible. TLC of whole brain gangliosides derivatized with orcinol-sulfuric acid reagent. The positions of the fractions on a parallel developed plate were determined; the TLC plates were attached directly to the MALDI target using double-sided adhesive tape or the strip cut from TLC plate. Different matrices were tested.

Composition and structures of tri-glycerides of human milk and some base components for infant milk formulas. Fat Sci. Technol. 96, 147-154 (1994). TLC of triglycerides, 1,2-, 1,3-,2,3-diglycerides, monoglyceride, and stearic acid on silica impregnated with boric acid solution (50g/L in ethanol) with acetone - water 3:2 and hexane - tert butyl-methyl-ether - acetic acid 40:60:1. Detection with 2',7'-dichlorofluorescein (200 mg/100 mL in ethanol). GC after extraction and esterification.

J. Planar Chromatogr. 9, 254-259 (1996). HPTLC of phospholipids - lysophosphatidylcholine, phosphatidylcholine, phosphatidylglycerine, phosphatidic acid, cholesterol, sphingomyelin, lysophosphatidylethanolamine, phosphatidylinositol, phosphatidylethanolamine - on silica with methyl acetate - chloroform - 1-propanol - methanol - 0.25% aqueous potassium chloride solution 25:25:25:10:9. Visualization under UV 254 and 366 nm and by immersion in an acidic solution of cupric acetate monohydrate. Quantification by densitometry at 350 nm. Detection limit of phospholipids approx. 10 - 1000 ng. Also separation of neutral lipids, cholesterol, and phospholipids with petrol ether - ether - acetic acid 40:40:1.

CBS 113, 13-15 (2014). HPTLC of glycosylceramide Glc-d18:2 h16:0 from wheat germ and standards squalene, cholesteryl oleate, glyceryl trioleate, linoleic acid, ß-sitosterol, and ß-sitosterol glucoside on silica gel in the AMD 2 with a 18-step gradient modified from Opitz et al. (Chromatographia 73 (2011) 559), methanol replaced ethanol, and the mobile phase composition was changed slightly (pre-conditioning with 4 M acetic acid before each step, drying time 1.5 min, development duration 3 h and solvent consumption 200 mL). Detection by dipping in copper sulfate phosphoric acid reagent for 20 s and heating at 130 °C for 15 min revealed grey-brown bands. Densitometry evaluation by absorbance measurement at 546 nm. For Glc-d18:2 h16:0, regression analysis showed a polynomial relationship with coefficients of determination (R2) from 0.995 to 0.999 (n=3, 50 - 1000 ng/band). LOD (S/N 3) and LOQ (S/N 10) of Glc-d18:2_x000D_ h16:0 were 10 ng/band and 50 ng/band, respectively (n = 6).

J. Chromatogr. Sci. 19, 530-535 (1983). TLC of fatty acid methylesters, triglycerides, fatty acids, diglycerides on silica with petrol ether -ether - acetic acid 90:10:1.

J. Anal. Chem. UdSSR 33, 830-832 (1983). TLC of trans-trans-2,4-hexadienoic acid, crotonylidene acetone, crotonic and acetic acids and diketene on silica with hexane - acetone 2:1. Detection with 0.15 N KMnO4.