coli by TLC with thionine as spot-test reagent. Recognition of cardiolipin, phosphatidylglycerol, phosphatidylethanolamine, and N-acyl phosphatidylethanolamine. J. Planar Chromatogr. 15, 23-27 (2002). One-and two-dimensional TLC of phospholipids (phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, and N-acyl phosphatidylethanolamine) on silica gel with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 for one-dimensional separation. Visualization by treatment for 20 min with aqueous thionine and differential staining with 0.05 M sulfurous acid. Additional phospholipid-profile information was obtained by separation on aluminium oxide with chloroform - methanol - 2-propanol - water 100:25:2:2 and staining with biebrich scarlet. 2D-TLC with 1-propanol - chloroform - ethyl acetate - methanol - water 50:50:50:21:18 in the first direction, treatment (in-situ) with 1% HCl and development with hexane - diethyl ether 5:1 in the second direction. Visualization with Schiff's leukofuchsin reagent.

CBS 81, 2-5 (1998). The performance and reliability of result of both procedures is comparable - HPTLC being slightly better than HPLC. For the assay of phospholipids in pure substances, pharmaceutical products and lecithin HPTLC is more cost efficient than HPLC by a factor of 2.5.

Anal. Chem. 75, 118-125 (2003). Online TLC separation and electrospray mass spectrometry (TLC/ESI-MS) by direct linking of a commercial overpressure TLC instrument, OPLC 50, and a Q-TOF mass spectrometer. Separation on silica gel with dichlormethane - methanol - water 60:35:8. A sensitivity of 5 pmol of glycosphingolipid was readily demonstrated for TLC/ESI-MS and 20 pmol for TLC/ESI-MS/MS production scanning to derive the saccharide sequence and long chain base/fatty acid composition of the ceramide. Initial preconditioning of TLC plates is necessary to achieve high sensitivity detection by reducing chemical background noise. Plates can be used repeatedly (at least 10 times) for analysis, although this may result in a minor reduction in TLC resolution. Following solvent development, separated components on the TLC plates can be detected in the conventional way by nondestructive staining or UV absorption or fluorescence and can be stored for on-line TLC/ESI-MS analysis at a later stage without reduction in mass spectrometric detection sensitivity and chromatographic resolution. Aspects for further improvement of OPLC instrumentation include use of narrower TLC plate dimensions and refined design of the eluate exit system.

J. Chromatogr. A 1572, 137-144 (2018). Development of a method for the analysis of anthocyanes within the foodstuffs of plant origin by TLC on RP-18 phase (which ensures mixed-mode retention mechanism with the localized adsorption on the non-bonded silanols) with acetic acid as the mobile phase component, using two anthocyanins (cyanin and keracyanin) and two anthocyanidins (pelargonidin and delphinidin) as phytochemical standards. By triple development, distinct and symmetrically shaped chromatographic zones were obtained. Further analysis of the products of hydrolytic degradation of the test anthocyanins by MS. Identification of the hydrolytically split fractions by using the p-aminobenzoic acid reagent. Investigation of the calibration curves for cyanin, keracyanin, pelargonidin and delphinidin, and the respective LOD and LOQ values. Application of the method to identify and quantify cyanin, keracyanin, pelargonidin and delphinidin in selected alimentary products (syrups, juices and herbal infusions).

J. Chromatogr. A 720, 3-25 (1996). A review with 234 references on the current improvement of TLC of gangliosides over the past decade, including basic general techniques and advice for separation of glycosphingolipids, new approaches concerning continuos and multiple development and preparative TLC and related techniques in practical applications.

CBS 114, 5-7 (2015). HPTLC of biodiesel (B100), biodiesel blended with diesel fuel in different proportions (BX, with X_x000D_ being the volume percent of B100 in the mixture) and standard monoacylglyceride on silica gel with the AMD 2 system in a 3-step development starting with 100 % t-butyl methyl ether (up to 40 mm), followed by dichloromethane – n-heptane 4:1 up to_x000D_ 60 mm and then 3:2 up to 90 mm as final migration distance. Detection by dipping in a 0.02 % methanolic solution of primuline. Quantitative determination of monoacylglycerides via the 1-oleyl glycerol standard by fluorescence measurement at 366/>400 nm. Elution of target zones with methanol, flow rate 0.2 mL/_x000D_min, via the TLC-MS Interface (oval elution head 4 x 2 mm) into an ion-trap MS operating in positive ESI mode. The final chromatogram showed a migration distance of 15-20 mm for the monoacylglyceride of BX, other lipids detected were diacylglycerides,_x000D_ triacylglycerides, fatty acids (20–60 mm) and fatty acid methyl esters (60–83 mm), the preponderant chemical class. The sample peak at 86 mm corresponded to the diesel component in the blend.

4. (Hungarian) (Margarine.) Determination of preserving matters content.) TLC of benzoic and sorbic acids on silica with benzene - dibutyl ether - acetic acid - formic acid 80:35:3:3. Detection by UV 254 nm.

Clin. Chem. 29, 250-255 (1983). TLC of lecithins with fatty acids of different chain length with chloroform - isopropanol - triethylamine -methanol -water 30:25:25:9:7. Detection with Cu2+/ H3PO4 spray. Densitometry.