J. Planar Chromatogr. 10, 308-310 (1997). TLC of diacylglycerols, cholesterol, free fatty acids and triacylglycerols on silica with hexane - ether - acetic acid 80:20:1. Detection by spraying with three different reagents: I. 3% cupric acetate in 6% aqueous phosphoric acid solution, followed by heating at 100°C for 10 min; II. 35% aqueous sulfuric acid solution, followed by heating at 140°C for 10 min; III. procedure I. followed by procedure II. Densitometry at 430 nm (absorbance). Also published in Proc. 9th Internat. Symp. Instr. Chromatogr., Interlaken, April 9.-11., 331-335 (1997).

J. Lipid Res. 38, 1482-1488 (1997). TLC of phospholipids and neutral lipids on EDTA-impregnated silica gel and after pre-concentration with chloroform - methanol - water 60:40:10 with five step-wise developments: i) chloroform - methanol - water 65:40:5 to 2 cm, ii) ethyl acetate - 2-propanol - ethanol - chloroform - methanol - 0.25% KCl 35:5:20:22:15:9 to 5 cm, iii) toluene -diethyl ether - ethanol 60:40:3 to 7.5 cm, iv) n-heptane - diethyl ether 94:8 to 10.5 cm, v) pure n-heptane to 12.5 cm. Charring by dipping in a solution of 10% cupric sulfate in 8% phosphoric acid for 10 s and heating at 200°C for 2 min. Quantitative determination with an image analyzer in transmission mode.

J. Lipid Res. 41, 142-147 (2000). 2-D TLC of lysophospholipids (lysophosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylinositol, lysophosphatidylserine, sphingosylphosphorylcholine, sphingosine-1-phosphate) on silica gel in the first direction with 1) chloroform - methanol - formic acid - water 60:30:7:3 and in the second direction with 2) chloroform - methanol - 28% NH3 - water 25:29:4:1. To separate lysophospholipids from neutral lipids on the same plate, a third run was performed with ether in the opposite direction to the second run.

J. Planar Chromatogr. 16, 405-407 (2003). HPTLC of neutral lipids (e.g. cholesteryl oleate, methyl oleate, triolein, oleic acid, and cholesterol) on silica gel (with prescored lanes), after prewashing with dichloromethane - methanol 1:1, for the methyl ester, triacylglycerol, free fatty acids, and free sterol content with petroleum ether - ether - acetic acid 80:20:1 and for cholesteryl esters with hexane - petroleum ether - ether - acetic acid 50:25:5:1. Development of plates in a presaturated chamber at 22°C and a humidity of 50%. Visualization by spraying with a solution of 5 g phosphomolybdic acid in 100 mL absolute ethanol and heating for 10 min at 115°C. TLC of polar lipids (cholesterol, phosphatidylethanolamine, phosphatidylcholine, and lysophosphatidylcholine) on silica gel with chloroform - methanol - water 65:25:4. Visualization by spraying with a 10% cupric sulfate solution (prepared by dissolving 100 g CuSO4 and 100 mL phosphoric acid in water and making up to 1 L), followed by heating for 10 min at 140°C. Quantitative densitometry at 610 nm for neutral lipids and at 370 nm for polar lipids.

J. Liq. Chromatogr. Relat. Technol. 28, 2571-2580 (2005). TLC of lipids, triacylglycerol, diacylglycerol, and sphingosine on silica gel with hexane - diethyl ether - formic acid 40:10:1 for neutral lipids, and with chloroform - methanol - water 65:25:4 for glycolipids. Phospholipids were separated by two dimensional development with chloroform - methanol - 28 % ammonium hydroxide 65:25:4 in the first direction, followed by drying and development with chloroform - acetone - methanol - acetic acid - water 30:40:10:10:1 in the second direction. Detection of neutral lipids by spraying with charring or phosphomolybdic acid reagent; detection of glycolipids by spraying with orcinol reagent (orcinol in 70 % sulfuric acid), and of phospholipids by spraying with molybdenum blue reagent for phosphate groups or ninhydrin reagent for phospholipids containing free amino groups.

J. Planar Chromatogr. 2, 24-27 (1989). HPTLC of glycerylmonostearate on silica with hexane - ethyl acetate - methanol 7:2:1. Postchromatographic derivatization by dipping into 0.05% methanolic solution of 6-(p-toluidino)-2-naphtalene-sulfonic acid (potassium salt) and then under UV 365 nm. HPTLC of glyceryl monodiricinolate on silica with ethyl acetate - dichloromethane - methanol - formic acid 13:6:1:0.1. Post-chromatographic derivatization by dipping into 3% ethanolic solution of molybdato phosphoric acid and heating to 120°C for 15 min.

J. Agric. Food Chem. 64, 1245-1255 (2016). Preparative HPTLC of gangliosides in the frontal lobe of piglets on silica gel with chloroform – methanol – calcium chloride 50:42:11. Fractions were subjected to a series of reactions to obtain sialic acid, glucose, galactose, N-acetyl-galactosamine, and fatty acid derivatives.

Anal. Biochem. 223, 232-238 (1994). Description of a method for purifying glycosphingolipids and phospholipids by using TLC blotting. Separation of glycosphingolipids by two-dimensional TLC and detection with primuline reagent. Transfer of the separated spots by TLC blotting to a polyvinylidene difluoride membrane, and extraction; monitoring by TLC.