Planta med. 65, 455-457 (1999). Preparative TLC on silica gel plates of 2-(4-hydroxyphenyl)ethyl 1-dodecyl-octadecanoate on silica gel with hexane - ethyl acetate 7:3 and of ursolic acid with chloroform - ethyl acetate 5:2. Detection under UV 254 nm.

J. Planar Chromatogr. 15, 463-465 (2002). HPTLC and TLC of 3,5-dihydroxybenzoic, vanillic, p-coumaric, p-hydroxybenzoic, gentisic, caffeic, syringic, sinapic, ferulic, protocatechuic, and 2,4-dihydroxybenzoic acid on silica gel and RP-18. Detection under UV 254 and 366 nm and by derivatization with bis-diazotized sulfanilamide. One-dimensional HPTLC was performed on RP-18 with methanol - water 2:3 and on silica gel with toluene - dioxane - formic acid 7:2:1 and a gradient of decreasing polarity and formic acid concentration. Documentation by densitometry. 2D-TLC on silica gel with toluene - dioxane - formic acid 7:2:1 in the first direction and, after drying, with a three step gradient elution program in the second direction. TLC on RP-18 was used in the first direction with methanol - water 2:3 After the first development the phenolic acids were transferred to the second silica gel plate with 70% methanol. TLC in the perpendicular direction was then performed with a three-step gradient elution program. The combined methods resulted in separation of all compounds investigated.

CBS 86, 4-5 (2001) HPTLC of 3-methoxy-4-amino azo meta sulphonic acid (MAMASA) on silica gel with n-butanol - diethylamine - ammonia - methanol 9:5:5:2 with chamber saturation for 5 min followed by drying at 40 °C. Quantitative determination by absorbance measurement at 254 nm.

J. Planar Chromatogr. 19, 200-203 (2006). TLC of 18 phenolic acids (salicylic, m-hydroxybenzoic, p-hydroxybenzoic, protocatechuic, alpha-resorcylic, beta-resorcylic, gallic, vanillic, syringic, gentisic, veratric, cinnamic, o-coumaric, m-coumaric, p-coumaric, caffeic, ferulic, and sinapic acid) an aminopropyl silica gel, prewashed with methanol and acetone, in a horizontal DS-chamber with mobile phases comprising mixtures of diisopropyl ether and acetic acid with toluene, petroleum ether, or heptane, partly with two developments. The best separations were obtained with heptane - diisopropyl ether - acetic acid 4:5:1, or petroleum ether - diisopropylether - acetic acid 6:3:1. Detection under UV light at 254 or 366 nm.

Chem.) 34, 707-711 (l985). (Japanese) (Determination of higher fatty acids in phospholipid subfractions of human platelets using thin-layer chromatography and gas chromatography.) TLC of phospholipid subfractions on silica with chloroform - methanol acetic acid - formic acid 50:30:4.5:6.5. Detection by spraying with 8-anilinonaphtalenesulfonic acid ammonium salt. Quantification by GC after extraction and methylation. Phospholipids were extracted from silica with chloroform - methanol 1:4 once and methanol twice. Methylation was done with boron trifluoride and methanol. The method is highly reliable.

J. Liq. Chromatogr. Relat. Technol. 30, 2267-2285 (2007). Analytical and preparative TLC of lipid classes, their fatty acid profiles, and the triacylglycerol and sterol composition on silica gel and modified silica gel (impregnated with silver nitrate for Ag-TLC or dimethyldichlorosilane for RP-TLC). TLC of lipid reference mixture on silica gel with hexane - acetone 25:4. Detection by spraying with 50 % ethanolic sulfuric acid and heating at 200 °C. Preparative TLC for isolation and quantification, followed by detection under UV light, spraying the edges with 2’,7’-dichlorofluorescein, scraping off, elution with diethyl ether and weighting. Quantitative Ag-TLC (impregnated by dipping into 0.5 % or 2 % methanolic solution of silver nitrate) followed by detection with bromine and sulfuryl chloride vapor for 30 min each, followed by heating at 180-200 °C. Preparative Ag-TLC with 4 different mobile phases. Quantitative RP-TLC on Kieselguhr treated for 6 h with vapors of dimethyldichlorosilane and washed with methanol using acetone - acetonitrile - water. Quantitative determination by absorbance measurement at 450 nm.

J. Planar Chromatogr. 26, 202-208 (2013). HPTLC of egg yolk lipid fractions on silica gel with hexane - diethyl ether - formic acid 40:10:1. Detection by srpaying with 10 % copper(II) sulfate in 8 % phosphoric acid. Quantitation by scanning with a flatbed scanner and a gel analysis software. Intermediate/interday/intra-day precision was below 10 % CV (n=6).

Marine Drugs 11 (2), 363-376 (2013). Lipid A fraction was isolated from Escherichia coli strain W3110 and from its plasmid-transformed derivatives HW000, HW001 and HW002 through an adapted three-step version of the Bligh and Dyer’s method. To control the transformation, TLC of the lipid extract, dissolved in chloroform – methanol 4:1, on silica gel with chloroform – methanol – water – ammonia 40:25:4:2. Detection by spraying with 10 % sulfuric acid in ethanol and heating at 175 °C; lipids were visualized as dark zones. Lipid A (hRf 40) was well separated from monophosphoryl lipid A (MPLA) (hRf 60) and from pentaacylated MPLA (hRf 50)._x000D_