J. Planar Chromatogr. 13, 228-231 (2000). HPTLC of neutral lipids (triacylglycerols, cholesteryl esters, free fatty acids and methyl esters) on silica gel using the Mangold system (petroleum ether - diethyl ether - acetic acid 80:20:1). Visualization by spraying with 5 % phosphomolybdic acid in ethanol and heating at 1108C for 15 min. Quantitation by densitometry at 700 nm.

Comparison of the hydrophobicity between Mycoplasma fermentans and human-brain lipids. J. Liq. Chromatogr. & Rel. Technol. 26, 1135-1147 (2003). HPTLC of glycolipids and phospholipids (phosphatidylcholine, sphingomyelin, phosphatidylethanolamine, lysophosphatidylethanolamine, lysophosphatidylcholine, cholesterol, cerebrosides) on silica gel with a mixture of chloroform, methanol, and 0.2% aqueous calcium chloride solution 15:8:1 and 11:9:2. Orcinal reagent and Dittmer's reagent were used for the detection.

J. Liq. Chromatogr. & Relat. Technol. 29, 2111-2127 (2006). TLC of phospho-lipids on silica gel with chloroform - methanol - water - formic acid 65:35:2:0.04. Detection by spraying with 2’,7’- dichlorofluorescein and under UV light. Phosphatidylserine and lyso-phosphatidylserine were detected by spraying with ethanolic ninhydrin reagent. TLC as screening method prior to the application to TLC-FID by the Iatroscan Chromarod system.

J. Agric. Food Chem. 33, 1093-1096 (1985). Two-dimensional TLC of radioactive total polar lipids on silica using chloroform - methanol - water 65:25:4 and chloroform - methanol - NH3 65:25:5 as eluents. Visualization with Dragendorff and ninhydrin reagents, also radioscanning.

PLoS ONE 8(3), e57908 (2013). Feline SD (Sandhoff disease) fibroblasts GM2 SV3 and human TSD (Tay-Sachs disease) glial cells were transfected to express hybrid subunits H1 and H2 of beta-hexosaminidase A (Hex) and grown for 18 h in medium containing 4.7 µg/mL NBD-GM2 (2-nitro1,3-benzoxadiazol fluorescent derivative of GM2 ganglioside, substrate of Hex) and 50 µM CBE (conduritol-B-epoxide). To check the transfection, HPTLC of glycosphingolipids, extracted according to Folch’s method into acidic and neutral glycolipids, on silica gel first with chloroform – acetone 1:1, followed by chloroform – methanol – 0.2% CaCl2 55:45:10; the separated gangliosides (NBD-GM2 and its breakdown NBD-products) were quantified by a fluor image analyzer (Storm Imager). Total ganglioside fractions obtained through ion-exchange chromatography from transfected SD lysates were incubated with NBD-GM2 and separated on HPTLC, with the same first mobile phase followed by chloroform – methanol – water 65:25:4; the reaction product NBD-GM3 appeared only when GM2 activator protein was added during the incubation.

J. Planar Chromatogr. 8, 362 - 365 (1995). HPTLC of macrophage and brain gangliosides on silica with chloroform - methanol- 0.02% aqueous calcium chloride 60:40:9. Visualization by spraying with resorcinol hydrochloride and heating at 110°C for 15 min. After the above described chromatographic separation the gangliosides were transferred from the silica gel layer to the PVDF membrane with the ganglioside electrotransfer technique. This enables easy determination of ganglioside structure by the application of specific antibodies.

Ghica Voda 41A, Iassy, Romania): Immunoassay detection of gangliosides by specific antibodies. CBS 94, 11-13 (2005). HPTLC of gangliosides extracted from tissues on silica gel with chloroform - methanol - 0.2% aqueous CaCl2 11:9:2 over 60 mm. Immunoassay detection by dipping in polyisobutylmethacrylate solution and bovine serum albumine solution, followed by immersion in anti-body containing supernatant or patient’s sera at 4° C overnight. After washing with phosphate-buffered saline detection of Mab binding by stepwise incubation with biotinylated chain-specific anti-mouse immunoglobulin, followed by steptavidin-horsradish peroxidase complex. Visualisation with chloro-4-naphtol reagent. Immuno detection is better than chemical derivatization with resorcinol-HCl reagent and has advantages over detection on ELISA microtiter plates.

CBS 105, 10-12 (2010). HPTLC of stratum corneum lipids (ceramides, cholesterol, phosphatidylcholine, squalene, sphingomyelin etc.) on silica gel by automated multiple development with a 8-step gradient from methanol to hexane in the AMD2 with pre-conditioning with 4M acetic acid before step 6. Detection by immersion in copper(II)sulfate reagent followed by heating at 170 °C for 8 min. Quantitative determination by absorbance measurement at 600 nm. Phosphatidylcholine and sphingomyelin remain at the start position, all other substances are separated.