Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      128 076
      A validated quantification of triclosan in toothpaste using high‑performance thin‑layer chromatography and a 48‑bit flatbed scanner
      B. ANDERS, S. DOLL, B. SPANGENBERG* (*Institute of Process Engineering, Offenburg University of Applied Sciences: Hochschule Offenburg, Badstrasse 24, 77652 Offenburg, Germany, spangenberg@HS-Offenburg.de)

      J. Planar Chromatogr. 34, 203-209 (2021). HPTLC of triclosan in toothpaste on silica gel with n-heptane - methyl tert-butyl ether - acetic acid 920:80:1. Detection by spraying with 2,6-dichloroquinone-4-chloroimide in 50 mL methanol, followed by spraying with an aqueous sodium carbonate solution (1 g/10 mL). Plates were scanned using a flatbed scanner. The hRF value for triclosan was 22. Linearity was between 100 and 1000 ng/zone. The LOD and LOQ were 46 and 91 ng/zone, respectively. Average recovery was 93.2 %.

      Classification: 28a
      127 038
      Chromatographic methods for the determination of aminexil, pyridoxine, and niacinamide in a novel cosmetic hair preparation
      M. REZK, H. ESSAM, E. AMER, D. YOUSSIF (*National Organization for Drug Control and Research, Wezaret El-Zeraa st., Dokki, Giza, Egypt, dmsy1982@yahoo.com)

      J. AOAC Int. 103, 1167-1172 (2020). HPTLC of aminexil (1), niacinamide (2) and pyridoxine HCl (3) on silica gel with propanol - toluene - 33 % ammonia solution
      20:30:1. Quantitative determination by absorbance measurement at 270 nm. Linearity was between 0.25 and 1.25 µg/zone for (1), 2 and 7 µg/zone for (2) and 3 and 7 µg/zone for (3). Intermediate precision was below 2 % (n=3). The LOD and LOQ were 20 and 60 ng/zone for (1), 180 and 540 ng/zone for (2) and 140 and 430 ng/zone for (3), respectively. Average recovery was 100.1 % for (1) and (2) and 101.1 % for (3). 

      Classification: 23
      123 050
      Direct bioautography hyphenated to direct analysis in real time mass spectrometry: Chromatographic separation, bioassay and mass spectra, all in the same sample run
      T. T. HÄBE, Maryam JAMSHIDI-AIDJI, Jennifer MACHO, Gertrud E. MORLOCK* (*Chair of Food Sci., Inst. of Nutrit. Sci., and Interdiscipl. Res. Center (iFZ), Justus Liebig Univ. Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany, Gertrud.Morlock@uni-giessen.de)

      J. Chromatogr. A 1568, 188-196 (2018). Application of an advantageous combination, the desorption-based direct analysis in real time mass spectrometry (DART-MS) immediately after direct bioautography (DB), i.e., in the presence of microorganisms, bioassay medium and substrate reagent. The method offers a straightforward and efficient mass spectrometric detection of bioactive analytes within the bioautogram. It discriminated microorganism cells and highly polar bioassay medium ingredients which could otherwise stress the MS system. Investigation of DB-DART-MS for bioactive compounds in cosmetics using the Bacillus subtilis and Aliivibrio fischeri bioassays for detection of Gram-positive and Gram-negative antimicrobials, respectively, and the planar yeast estrogen screen for detection of estrogen-effective compounds. Study of the influences of three different bioassay matrices on the analyte response and DB-DART-MS performance on different layers (NP and RP) on the example of parabens in hand creams. Ion suppression was enhanced with increasing culture medium complexity. The mass spectrometric quantification by DB-DART-MS at the ng-level in situ each different bioautogram was verified by comparison to HPTLC-DART-MS. The total paraben content of hand creams 1 and 2 was 0.17–0.20% and 0.30–0.34%, respectively, depending on the method used. It proved that DB-DART-MS is a reliable qantitative bioanalytical hyphenation.

      Keywords: cosmetics HPTLC
      Classification: 4e
      108 105
      Development and validation of HPTLC densitometric method for identification and quantification of geraniol in Palmarosa oil
      A. LEHRI, J. BARTHWAL, A. NIRANJAN*, D.V. AMLA (*Central Instrumentation Facility, National Botanical Research Institute, Council of Scientifc and Industrial Research, Lucknow-226 001, India; abishek_niranjan@yahoo.co.in)

      J. Planar Chromatogr. 24, 316-319 (2011). HPTLC of Palmarosa oil extracts in toluene and geraniol on silica gel with toluene - ethyl acetate 37:3 in a twin-trough chamber saturated for 30 min at 25 +/- 2 °C. Detection by spraying with 3 % vanillin in ethanol - sulfuric acid 49:1 followed by heating at 100 °C for 5 min. Quantitative determination by densitometry in absorption mode at 400 nm. The instrumental precision and the repeatability (n = 6), was 0.3 and 3.2 %, respectively. LOD and LOQ was 1.4 and 2.8 µg/mL, respectively. The intra-day recovery was 99.8 % and the inter-day recovery 99.4 %. The hRf value for geraniol was 36.

      Classification: 32e
      117 071
      Propolis based lipid nanoparticles as carriers of cosmetic actives – Development, characterization and safety evaluation
      Y. CORREA, D. PAILLIE, A. VALENZUELA, M. ROJAS, Claudia MORA* (*National University of Colombia, Faculty of Sciences, Department of Pharmacy, Bogota, Colombia, cemorah@unal.edu.co)

      Rev. Colomb. Cienc. Quim. Farm. 45, 147-168 (2016). HPTLC of propolis based lipid nanoparticles containing tea tree (Melaleuca alternifolia) on silica gel with toluene – ethyl acetate 93:7. Qualitative determination under UV light at 365 nm. Detection by spraying with anisaldehyde-sulfuric acid reagent (0.5 mL anisaldehyde was mixed with 10 mL glacial acetic acid, followed by 85 mL methanol and 5 mL concentrated sulfuric acid).

      Classification: 11c
      77 084
      Determination of allantoin in biological, cosmetic, and pharmaceutical samples
      X.B. CHEN, W. MATUSZEWSKI, J. KOWALZYK, (Rowett Research Inst., Bucksburn, Aberdeen AB2 9SB, UK)

      J. AOAC Int. 79, 628-635 (1996). TLC of allantoin in pharmaceuticals on silica with e.g. methanol - acetone - formic acid - water 40:2:1:6 and in urine on cellulose with butanol - acetic acid - water 2:1:1. Detection with pDMAB (Ehrlich's reagent). Quantification by densitometry at 445 nm. TLC as fast and inexpensive semiquantitative method for estimation of allantoin in natural samples.

      Classification: 23e
      108 133
      Normal-phase TLC analysis of UV filter avobenzone and octocrylene in sunscreen preparations
      Anna SOBANSKA*, E. BRZEZINSKA (*Department of Analytical Chemistry, Medical University of Lodz, UL. Muszynskiego 1, 90-151 Lodz, Poland; a.sob@poczta.onet.pl)

      J. Planar Chromatogr. 24, 154-159 (2011). TLC of avobenzone (methoxydibenzoylmethane), octocrylene, Uvinul T 150 (ethyl hexyl triazone, ET), and ethylparaben on silica gel with cyclohexane - diethyl ether 1:1 (method A), or with cyclohexane - piperidine 15:1 (method B), in a chamber lined with filter paper and saturated for 20 min. Quantitative determination by densitometry at 380 nm for avobenzene and at 300 nm for octocrylene. LOD and LOQ were 170 and 510 ng/zone for avobenzone (method A) and 180 and 537 ng/zone for octocrylene (method B), respectively. The linearity range was 200-1800 ng/zone for avobenzone and octocrylene.

      Classification: 32a
      117 076
      Analysis of plant glycosylceramides by automated multiple development
      M. REISBERG*, R. NEUBERT, Birgit DRAEGER (*Institute of Pharmacy, Martin-Luther-
      University Halle-Wittenberg, 06099 Halle, Germany, mathias.reisberg@pharmazie.uni-halle.de)

      CBS 113, 13-15 (2014). HPTLC of glycosylceramide Glc-d18:2 h16:0 from wheat germ and standards squalene, cholesteryl oleate, glyceryl trioleate, linoleic acid, ß-sitosterol, and ß-sitosterol glucoside on silica gel in the AMD 2 with a 18-step gradient modified from Opitz et al. (Chromatographia 73 (2011) 559), methanol replaced ethanol, and the mobile phase composition was changed slightly (pre-conditioning with 4 M acetic acid before each step, drying time 1.5 min, development duration 3 h and solvent consumption 200 mL). Detection by dipping in copper sulfate phosphoric acid reagent for 20 s and heating at 130 °C for 15 min revealed grey-brown bands. Densitometry evaluation by absorbance measurement at 546 nm. For Glc-d18:2 h16:0, regression analysis showed a polynomial relationship with coefficients of determination (R2) from 0.995 to 0.999 (n=3, 50 - 1000 ng/band). LOD (S/N 3) and LOQ (S/N 10) of Glc-d18:2_x000D_ h16:0 were 10 ng/band and 50 ng/band, respectively (n = 6).

      Classification: 11