J. Chinese Herb Med. (Zhongcaoyao) 26, 258-260 (1995). TLC of oleanolic acid on silica with chloroform - methanol 20:1. Detection by spraying with 10% sulfuric acid in ethanol. Quantification by densitometry at 530 nm.

Chromatographia 45, 195-198 (1997). 2D-TLC of amino acids, sugars, oligosaccharides, and organic acids on silica and cellulose with different solvents. Detection with suitable spray reagents. Application of the technique for diagnosis of inherited metabolic diseases with some pathological examples.

Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 231-235. Validated HPTLC on silica gel with ethyl acetate - methanol - acetic acid 80:19:1 for ca. 15 min. Quantitation by absorbance measurement at 300 nm (polynomial regression). Recovery was found to be between 99,0 and 101,3 %, repeatability was 0,1 % for aspirin and 2,2% for salicylic acid (n=2). HPTLC method is preferred to HPLC method because of its speed (14 samples in 30 min).

J. Planar Chromatogr. 15, 4-10, (2002). HPTLC of flavones and flavanones (myricetin, luteolin, apigenin, acacetin, hyperoside, quercetin, rutin, quercitrin, astragalin, kaempferol, isoquercitrin, naringenin, naringin, hesperitin, hesperidin) and phenolic acids (caffeic, ferulic, and chlorogenic acid) on CN-(cyanopropyl)-modified silica gel with e.g. 60% acetone in n-hexane for the development in the first direction and 50% methanol in water for the development in the second direction. Visualization with poly(ethylene glycol) 400 and 2-(diphenylboryoxo)ethylamine. Documentation at 366 nm.

Part I. Comparison of separations of fatty acids on RP-18 plates with and without a concentrating zone. J. Planar Chromatogr. 15, 332-340 (2002). TLC separation of 19 on RP 18 with and without concentrating zone with methanol - water 9:1, 19:1 and methanol in a chamber saturated for 30 min. Visualization by exposure to iodine vapor. The visual inspection of the chromatograms revealed that better separation was achieved on plates with concentrating zones because compact chromatographic bands were obtained. The resolution values RS calculated confirmed these observations.

J. Planar Chromatogr. 17, 51-53 (2004). TLC of gallic acid, salicylic acid and tannic acid on silica gel with chloroform - ethyl acetate - formic acid 5:4:1. Detection with the iron(III) - 2,2’-dipyridyl system (iron ammonium sulfate and sulfuric acid in water and 2,2’-dipyridyl in hydrochloric acid). Quantitative determination at 280 nm. Limit of detection 0.1 µg per spot.

Retention behaviour on polar adsorbents (silica, alumina, polyamide) with non-aqueous mobile phases. Acta Chrom. 9, 38-54 (1999). TLC of benzoic and cinnamic acid derivatives on silica gel 60 H compared with that on alumina oxide 60 G and polyamide layers with non-aqueous ternary mobile phases comprising a non-polar diluent (n-heptane), a polar modifier (2-propanol, dioxane, tetrahydrofuran and ethyl acetate) and 2 % acetic acid in horizontal chambers. Visualization under UV 254 nm, and by spraying with diazotized sulphanilic acid in 10 % sodium bicarbonate solution, or with a 2 % solution of ferric chloride. Chromatogram parameters and the correlation diagrams were used for comparison of separation selectivity.

J. Agric. Food Chem. 34, 688-694 (1986). Determination of alfaprostol and metabolites on silica with benzene - dioxane - acetic acid 20:20:1. Detection by spraying with phosphomolybdic acid and heating. Detection of radioactive zones with a radiochromatogram spark chamber.