J. Planar Chromatogr. B, 155-158 (1999). HPTLC of neutral lipids (cholesteryl oleate, methyl oleate, triolein, oleic acid, and cholesterol) on silica gel with petroleum ether - diethyl ether - acetic acid - 40:10:1. To test for the presence of cholesteryl esters, plates were developed with n-hexane - petroleum ether - diethyl ether - acetic acid 50:20:5:1. All developments were performed at 20°C and 60% relative humidity in a vapor-equilibrated, paper-lined chamber. Neutral lipids were detected as blue zones on a yellow background by spraying with 5% ethanolic phosphomolybdic acid and drying in an oven at 110°C for 15 min. Densitometry of neutral lipid zones at 700 nm. TLC of phospholipids on silica gel with chloroform - methanol - water 65:25:4; detection as black zones on a white background by spraying with 10% copper(II) sulfate in 8% phosphoric acid reagent and heating at 140°C for 15 min. Quantitation by densitometry at 370 nm.

J. Planar Chromatogr. 13, 375-381 (2000). HPTLC of 19 lipids on silica gel with petroleum ether (40-608C) - ether - acetic acid in the proportions 15:5:3; 80:20:3; 40:10:1; 40:10:3; 20:5:2. Visualization by exposure to iodine vapor or by use of a charring reagent comprising 1 g manganese chloride, 150 mL methanol, and 10 mL concentrated sulfuric acid in 150 mL of deionized water, followed by heating at 110°C for precisely 45 min. Quantitation by densitometry.

Proc. Intern. Symp. on Planar Separations Plan. Chrom. 267-273 (2003). TLC of phospholipids (i.a. phosphatidylinositols, -cholines, -serines, -ethanolamines, -glycerines, neutral lipids) on silica gel with chloroform - methanol - water 65:25:4 and chloroform - methanol - acetic acid 65:25:8. After drying 5% phosphomolybdic acid was used for spraying or immersion; also iodine vapor, Dragendorff, butanolic ninhydrin solution and ammoniacal silver nitrate solution. Quantitation by densitometry. Effective quantitative analysis.

J. Liq. Chromatogr. & Relat. Technol. 29, 2167-2180 (2006). HPTLC of neutral lipids on prewashed silica gel with petroleum ether - diethyl ether - glacial acetic acid 80:20:1; and of methyl and steryl esters with hexane - petroleum ether (35-60°C) - diethyl ether - glacial acetic acid 50:25:5:1 in a twin trough chamber saturated for 20 min. Detection by spraying with a 50 % solution of phosphomolybdic acid in ethanol, followed by heating at 115°C for 10 min. Polar lipids were separated with chloroform - methanol - water 65:25:4. Detection by spraying with 10 % cupric sulfate solution, followed by heating at 140 °C for 10 min. Quantitative determination by absorbance measurement at 610 nm for neutral lipids and at 370 nm for polar lipids.

I. Separation and identification of prostaglandin A1 from the common onion, Allium cepa.) (Chinese). J. Chinese Herb. Med. 19, 146-147 (1988) (Zhongcaoyao). Preparative TLC on silica with chloroform - THF - acetic acid 10:2:1. Elution with chloroform - methanol 1:1. Identification by UV, IR, NMR, MS.

Biochem. Biophys. Res. Commun. 470, 168-174 (2016). HPTLC of glycosphingolipid during the evaluation of enzyme activities of human Gb3/CD77 synthase supplemented with UDP-[14C]Gal on silica gel with chloroform – methanol – water 11:9:2. Detection of radiolabeled reaction products by exposure to phosphor screens for 8 weeks in -80 °C, followed by scanning. In parallel staining was used to facilitate identification of bands on the phosphor screens by spraying the plates with orcinol (0.2 %) in 3 M aqueous sulfuric acid, followed by heating at 110 °C for 10 min.

(German). Fat Sci. Technol. 97, 229-235 (1995). TLC of membrane phosphatides (sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, cardiolipin) on silica with chloroform - methanol - acetic acid - water 30:15:4:2. Detection by spraying with 0.05% rhodamin 6G in acetone. TLC of acetylated diglycerides on silica impregnated with 10% aqueous silver nitrate solution and chloroform - methanol 99:1 or chloroform - acetic acid 19:1. In addition TLC on RP-18 with acetone - acetonitrile - chloroform 5:4:2. Densitometry.

CBS 105, 10-12 (2010). HPTLC of stratum corneum lipids (ceramides, cholesterol, phosphatidylcholine, squalene, sphingomyelin etc.) on silica gel by automated multiple development with a 8-step gradient from methanol to hexane in the AMD2 with pre-conditioning with 4M acetic acid before step 6. Detection by immersion in copper(II)sulfate reagent followed by heating at 170 °C for 8 min. Quantitative determination by absorbance measurement at 600 nm. Phosphatidylcholine and sphingomyelin remain at the start position, all other substances are separated.