Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.

      Optimized determination of caffeine, equol, and artemisinin by High-Performance Thin-Layer Chromatography–direct analysis in real time–time of flight–mass spectrometry
      F. EICHNER, B. SPANGENBERG* (*University of Applied Sciences Offenburg, Department of Process Engineering, Badstrasse 24, 77652 Offenburg, Germany, Spangenberg@HS-Offenburg.de)

      J. Planar Chromatogr. 32, 197-203 (2019). HPTLC of equol (1), artemisinin (2) and caffeine (3) on silica gel with methyl t-butyl ether - cyclohexane 1:1 for (1), cyclohexane - ethyl acetate 7:3 for (2) and ethyl acetate - acetone 7:3 for (3). The plate was scanned with a DART–TOF MS system to optimize the measurement conditions. The hRF values for (1) to (3) were 71, 63 and 53, respectively. LOD and LOQ were 1.2 and 1.8 µg/zone for (1), 225 and 315 ng/zone for (2) and 270 and 490 ng/zone for (3), respectivley.

      Classification: 3f
      100 013
      New coupling of planar chromatography with direct analysis in real time mass spectrometry
      Gertrud MORLOCK*, Y. UEDA (*Institute of Food Chemistry, University of Hohenheim, Garbenstrasse 28, D-70599 Stuttgart, Germany)

      J. Chromatogr. A 1043 (1-2), 243-251 (2007). Presentation of the coupling of planar chromatography with direct analysis in real time time-of-flight mass spectrometry (DART-TOF-MS) for the first time. By cutting the plate within a track led to substance zones positioned on the plate edge, the interested zones were directly introduced into the DART gas stream to obtain the mass signals instantaneously within seconds, giving the detectability in the very low ng/zone-range on the example of isopropylthioxanthone. The coupling was perfectly suited for identification and qualitative purposes, but for quantification of results the analytical response and the repeatability were strongly dependent from proper manual positioning of the HPTLC plate into the excited-state gas stream of the ion source. By using stable isotope-labeled standards the drawback can be overcome demonstrated with the example of caffeine, and the analytical response (r2 of 0.9892) and repeatability (RSD < ±5.4%, n = 6) were improved to a high extent. The spatial resolution by an in-house-built plate holder system was shown to be better than 3 mm; the decay of the signal was observed. Comparison of the efficacy of this new coupling to a plunger-based extraction device for HPTLC/electrospray ionization–MS. The detectability of latter showed to be down to the pg/zone-range, e.g. the limit of quantification for isopropylthioxanthone to be 100 pg/zone. The repeatability was comparable (RSD ± 6.7%), however, without the need of internal standard correction, and the analytical response slightly better (r2 of 0.9983). The spatial resolution was 2 mm or 4 mm depending on the plunger head used.

      Classification: 3f
      116 015
      A simple device for the solute elution from thin-layer
      chromatographic plates to high-performance liquid chromatographic or mass spectrometric instrument
      Anna TUREK*, P. ZYCHON, A. CHOMICKI, T. DZIDO (*Department of Physical
      Chemistry, Chair of Chemistry, Medical University, Lublin, Poland, anna.klimek@am.lublin.pl)

      J. Planar Chromatogr. 28, 402-406 (2015). Design of low-cost and relatively simple TLC-HPLC-MS set-up for sample elution from TLC plate to the HPLC or MS system. A prototype eluting device with a tubing for direct solute zone collection was constructed. The tubing consisted of a stainless steel capillary into which a Teflon capillary was placed. At the lower bottom of the stainless steel capillary the tip of the Teflon capillary formed a 3 mm deep dimple and the capillary walls were sharpened. The stainless steel capillary was disconnected from the prototype and by pressing the sharpened end on blotting paper a disc was resected which was located directly on the Teflon tip. Then the capillary was pressed against the adsorbent layer with the analyte zone. An advantage of the prototype was that this yould be done directly under the UV lamp without the need to mark the analyte zones on the layer. Like this a chip with the adsorbent layer was introduced into the capillary dimple. Then, the chip was covered with another disc of blotting paper (cut using the sharpened capillary). Subsequently, the stainless steel capillary was connected with the prototype, the injection valve was switched and the eluent flow was directed through the stainless steel capillary to the UV–VIS or MS detector. After the elution, the injection valve was switched again, the capillary was disassembled from the prototype and the remnant of the adsorbent layer was removed so that the capillary was ready for the next elution. The results obtained using the prototype device were compared to those obtained with the commercial TLC-MS Interface manufactured by CAMAG. Regardless of the device used the peak areas of the eluted analytes were correlated to their concentrations. However, the average peak area obtained with the TLC-MS Interface was considerably higher than that obtained with the prototype because the diameter of the tip of the stainless steel capillary was only 1 mm compared to the oval elution head (2x4 mm) of the TLC-MS Interface.

      Keywords:
      Classification: 3f, 4e
      58 025
      Quantitation of thin-layer chromatograms with an Apple II computer-based video densitometer
      T.S. FORD-HOLEVINSKI, N.S. RADIN

      Anal. Biochem. 150, 359-363 (1985). Description of a high-speed densitometer consisting of an Apple II computer, a black-and-white video camera, and an image-digitizing board. Measurement of a spot in 30 -40 sec. by supplementing the computer with a very fast coprocessor. R.S.D. for multiple readings of a 500 ng spot of charred lipid 0.5 % ..

      Keywords:
      Classification: 3f
      63 011
      Scanning TLC plates in the fluorescence mode
      E.W. BERKHAN, (D-6940 Weinheim, FRG)

      J. Planar Chromatogr. 1, 81-85 (1988). Discussion of the effects of instrumental parameters and properties of TLC plates on the sensitivity and reproducibility of fluorescence scanning detection, and of the effect of some noninstrumental parameters including fluorescent indicators and the fluorescent background from derivatization and textile fibers, etc.

      Keywords: densitometry
      Classification: 3f
      65 028
      Quantitative analysis of anabolics on thin-layer chromatographic plates using image analysis techniques
      E.H.J.M. JANSEN*, D. VAN DEN BOSCH, R.W. STEPHANY, L.J. VAN LOOK, C. VAN PATEGHEM, (*Nat. Inst. Pub. Health & Environ. Protection, P.O. Box 1, 3720 BA Bilthoven, The Netherlands)

      J. Chromatogr. 489, 205-212 (1989). Introduction of a new detection system for the quantitative TLC analysis based on a relatively simple image analysis system. Applications for a number of samples containing anabolic compounds.

      Keywords:
      Classification: 3f
      68 046
      Sources of error in the densitometric evaluation of thin-layer separation with special regard to nonlinear problems
      V.A. POLLAK, (Dep. Electr. Eng., Univ. Saskatchewam, Saskatoon, SK Canada)

      Adv. Chromatogr. (N.Y.) 30, 201-222 (1989). A review with 15 references on the error sources in densitometric evaluation of thin-layer chromatograms and electrophorograms, one- and two-dimensional versions, turbid and transparent media, comparison of slit scanning and spot scanning. Discussion of baseline, low concentration separations and gain correction.

      Keywords: review
      Classification: 3f
      74 026
      Image analysis of photochemically derivatized and charge-coupled device-detected phenothiazines separated by thin-layer chromatography
      T.G. SANCHEZ*, A.N. DIAZ, M.R. CORREA, (*Dept. Anal. Chem., Fac. Sci., Univ. Malaga, 29071 Malaga, Spain)

      J. Chromatogr. 655, 31-38, (1994). TLC of phenothiazines on silica with methanol - acetic acid 955. Detection by photochemical derivatization. Identification and quantification of propiomazine, acetopromazine and chlorpromazine by image analysis with a CCD camera, using both pseudocolor red-greenblue and grey-scale tones.

      Classification: 3f