J. Planar Chromatogr. 17, 164-168 (2004). HPTLC of flupirtine maleate on silica gel with ethyl acetate - methanol - 25 % ammonia 17:2:1 in a saturated developing chamber. Then the developed plate must be dipped for 2 s in 1:3 paraffin - hexane. Dipping increases the fluorescence tenfold and preserves the fluorescence stability for hours. Presentation of a new formula for transforming fluorescence measurements in accordance with Kubelka-Munk theory. The fluorescence signals, the absorption signals, and data from a selected reference are combined in one expression. Only diode-array techniques can measure all the required data simultaneously. The fluorescence calibration curve was linear over the range 300 to 5000 ng per spot.

Phytochem. Anal. 24, 303-308 (2013). HPTLC of chicoric acid (1), chlorogenic acid (2) and echinacoside (3) in commercial Echinacea products on silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:17. Qualitative identification under UV 366 nm. Images were quantified and transformed into chromatograms, as signal intensities (peaks) versus hRf (retention factor) for a artificial neural network (ANN) correlation. LOD were 19, 46 and 29 ng/band, whereas LOQ were 63, 154 and 98 ng/band for (1), (2) and (3), respectively.

R.E. Kaiser (Ed) "Proc. of the 3rd Int. Symp. on Instr. HPTLC, Wuerzburg" IfC, Bad Duerkheim 1985, 373-380.Description of the method,, which can be used for detection, identification and quantification in both absorbance and fluorescence. Application example from drug analysis (anthraquinone type, laxative).

Anal. Sci. 3, 11-15 (1987). Description of a photoacoustic densitometry technique by laser beam scanning on a photoacoustic cell without chopping or modulation. Conversion and processing of the photoacoustic signals by microcomputer. The reproducibility of measurement for 0.03 to 0.25 µg Malachite Green was 1.7-4.5% RSD.

Realised in the systems containing binary and ternary mobile phases. Annales Universitatis Mariae Curie- Skodowska XLI 11, 120-127 (1986). Description of a graphical method for the evaluation and comparison of the resolution obtained with binary and ternary solvent mixtures. It is based on position, shape and size of two neighbouring spots and serves for the optimization of the solvent ratio. The method is demonstrated by TLC of aromatic hydrocarbons on silica with mixtures of carbon tetrachloride, benzene, chloroform, dioxane and acetone.

Chem. Anal. (N.Y.) 108, 185-196 (1990). Studies of the measurement of two-dimensional gel electrophoretic and HPTLC patterns. Presentation of two qualitative mapping techniques. Examination of quantitative analysis of partial spots using calibration curves.

Anal. Chem. 64, 3176-3179 (1992). Description of a square-wave anodic stripping voltametric method for ppm determination of heavy metals. Separation by TLC on carboxymethyl cellulose using a cellulose dialysis membrane - covered mercury film electrode placed directly on the TLC plate surface in a thin-film of supporting electrolyte solution. Presentation of the results for a mixture of Pb(II), Cd(II), and Zn(II). Calibration curves for Pb(II).

Chromatogram evaluation using video systems. BioTec 2, 28-30 (1997). State of modern video evaluation is given. The same dyestuff chromatogram is evaluated in UV and white light once by a conventional scanner and once by an electronical video system. The results are comparable. Advantages of video systems are: fast evaluation, low additional cost (if an video documentation system is already available) and later quantification of formerly documented chromatograms. Advantages of conventional scanners are: spectral selectivity, use of the whole UV range up to 190 nm, spectra recording and often a slightly better precision. (Note: The paper is not correctly reproduced by the journal - please order it from the authors directly.)