Acta Biologica Hungarica 36, 45-70 (1985). Sexual steroids extracted from plasma samples with ether. TLC of testosterone, progesterone, l7 beta-estradiol on silica with dichloromethane - ethyl acetate 8:2 for testosterone, chloroform - ethyl acetate 1:50 for progesterone and chloroform - ethanol 9:1 for 17 beta -estradiol. Detection by UV for testosterone, with 2,4-dinitrophenylhydrazine for progesterone and 1:1 mixture of FeCl3 and K3Fe(Cl)6 for estradiol.

J. Planar Chromatogr. 3, 189-190 (1990). HPTLC of clioquinol, hydrocortisone and hydrocortisone acetate on silica with hexane - ethyl acetate - acetic acid 20:30:1 after washing the layers with dichloromethane - methanol 1:1 prior to sample application. Quantification by densitometry.

J. Chromatogr. A 1530, 185-191 (2017). Development of a new spray-on method for applying yeast cells to HPTLC plates, leading to a much higher sensitivity of the planar yeast estrogen screen (p-YES), which can serve as a highly valuable and sensitive screening tool for the detection of estrogenic compounds in various sample matrices such as water and wastewater, personal care products and foodstuff. HPTLC of sample constituents and direct detection of estrogenic compounds by spraying with yeast cells. This resulted in much sharper signals compared to those in previous publications. Satisfying results were achieved using cultures with cell densities of 1000 FAU with reduced signal broadening, thus lower LOQ for estrogenic compounds, e.g. estrone 2 pg/zone, 17β-estradiol 0.5 pg/zone, 17α-ethinylestradiol 0.5 pg/zone and estriol 20 pg/zone. Demonstration of the possibility of the method to characterize profiles of estrogenic activity of wastewater samples with high quality and reproducibility by using native samples from wastewater or even surface water directly applied on HPTLC plates without the need for prior sample treatment.

Food Chem. 221, 1232-1244 (2017). Review of novel analytical methods for the detection of milk adulterants, including the use of RP-TLC for the determination of vegetable oils as adulterant of fat content. In this methodology, vegetable oil content was quantified by monitoring the structure of sterols by using β-sitosterol as a marker.

J. Planar Chromatogr. 31, 332-336 (2018). HPTLC of β-sitosterol (1) and lupeol (2) in Saussurea lappa and Saussurea auriculata on silica gel with toluene ‒ ethyl acetate ‒ glacial acetic acid 29:9:2. Detection by spraying with anisaldehyde–sulfuric acid reagent. Quantitative determination by absorbance measurement at 525 nm. The hRf values for (1) and (2) were 71 and 88, respectively. Linearity was between 100 and 400 ng/zone. LOD and LOQ were 7 and 23 ng for (1), and 6 and 17 ng for (2), respectively. The intermediate precision was <2 %. Average recovery was 99.9 % for (1) and 100.0 % for (2).

J. A.O.A.C. 70, 499-501 (1987). TLC determination of coprostanol and cholesterol on silica, impregnated with 5 % alcoholic phosphomolybdic acid solution, with ether - heptane 55:45. Detection by heating for 20 min at 120 °C.

J. Liquid Chromatogr. 12, 3151-3161 (1989). TLC of sterols and other lipids on silica with isopropyl ether – acetic acid 96:4, followed by hexane – ether – acetic acid 90:10:1. Detection of lipids by spraying with 5% ethanolic phosphomolybdic acid and heating for 5-10 min at 100-120°C. Semiquantification by comparison of the zone sizes and intensities of standards and samples. Quantification of cholesterol by densitometry after development with chloroform – ethyl acetate 96:4, and dipping in cupric acetate – phosphoric acid reagent and heating at 100°C. Quantification of triacylglycerols and free fatty acids by densitometry after development with hexane – ether – acetic acid 80:20:1, followed by spraying with cupric acetate – phosphoric acid reagent. Also GC.

Anal. Chim. Acta 275, 177-182 (1993). HPTLC of corticosteroids on silica with chloroform - methanol 98:2. Detection by spraying with a mixed solution of 1% sulfuric acid in acetic acid, and heating for 10 min. at 95 °C. Examination in daylight and under UV 366 nm.