Phytochemistry 23, 73-82 (1984). TLC of Zea mays sterols on silica or silica containing 10 % of silver nitrate with a) chloroform - ether 49:1, b) chloroform - ether 97:3. Detection by UV after spraying with 0.005 % berberine HCl in ethanol. Characterization by HPLC and GC/MS.

(Japanese). Bunseki 9, 647-656 (1988). A review with 12 references.

J. Planar Chromatogr. 4, 255-257 (1991). HPTLC of progesterone, testosterone, testosterone hydrogen sulfate sodium salt, 20ß-hydroxy-4-pregnene-3-one, 2-methoxy estrone, coprostane, hydrocortisone on silica with a gradient consisting of methanol – ethyl acetate – chloroform – methylene chloride (first inverse gradient program) and of methanol – chloroform (second inverse gradient program). Detection under UV 254 nm. Quantification by densitometry.

J. Chromatogr. 674, 247-353 (1994). HPTLC of anabolic steroids on silica with chloroform - acetone 9:1 in one direction and cyclohexane - ethyl acetate - methanol 117:78:11 in the opposite direction. Detection by spraying with 10% sulfuric acid in methanol and heating for 10 min. at 95 °C. Observation in daylight and under UV 366 nm. Confirmation of the identities by GC-MS.

Chromatographia 63 (7-8), 383-388 (2006). Specific and rapid determination of free cortisol and cortisone in human urine has been achieved by concentration of the urine samples, liquid-liquid extraction of the concentrated samples, TLC of ethanolic extracts on silica gel, location of the steroids under UV light, elution of cortisol and cortisone from sections of the plates with phosphate buffer, and measurement by competitive protein-binding assay. Chicken serum was used as the source of corticosteroid binding globulin, because it binds cortisol and cortisone with similar high affinity. The method combining TLC and competitive protein-binding assay is easy to perform, specific, sensitive, and quite rapid. Free cortisol and cortisone were measured in the urine of male individuals who abstained from water intake for 2 h or who ingested 1 L of water. The results show, for the first time, that short-term water diuresis markedly increases urinary free cortisone excretion, supporting our previous hypothesis that its excretion is positively correlated with urine volume, i.e. with the volume of 24-h urine samples.

Acta Biologica Hungarica 36, 45-70 (1985). Sexual steroids extracted from plasma samples with ether. TLC of testosterone, progesterone, l7 beta-estradiol on silica with dichloromethane - ethyl acetate 8:2 for testosterone, chloroform - ethyl acetate 1:50 for progesterone and chloroform - ethanol 9:1 for 17 beta -estradiol. Detection by UV for testosterone, with 2,4-dinitrophenylhydrazine for progesterone and 1:1 mixture of FeCl3 and K3Fe(Cl)6 for estradiol.

J. Planar Chromatogr. 3, 189-190 (1990). HPTLC of clioquinol, hydrocortisone and hydrocortisone acetate on silica with hexane - ethyl acetate - acetic acid 20:30:1 after washing the layers with dichloromethane - methanol 1:1 prior to sample application. Quantification by densitometry.

J. Chromatogr. A 1530, 185-191 (2017). Development of a new spray-on method for applying yeast cells to HPTLC plates, leading to a much higher sensitivity of the planar yeast estrogen screen (p-YES), which can serve as a highly valuable and sensitive screening tool for the detection of estrogenic compounds in various sample matrices such as water and wastewater, personal care products and foodstuff. HPTLC of sample constituents and direct detection of estrogenic compounds by spraying with yeast cells. This resulted in much sharper signals compared to those in previous publications. Satisfying results were achieved using cultures with cell densities of 1000 FAU with reduced signal broadening, thus lower LOQ for estrogenic compounds, e.g. estrone 2 pg/zone, 17β-estradiol 0.5 pg/zone, 17α-ethinylestradiol 0.5 pg/zone and estriol 20 pg/zone. Demonstration of the possibility of the method to characterize profiles of estrogenic activity of wastewater samples with high quality and reproducibility by using native samples from wastewater or even surface water directly applied on HPTLC plates without the need for prior sample treatment.