Anal. Chim. Acta 275, 147-162 (1993). HPTLC of the anabolic residues in biological samples on silica with seven solvent systems in one or in two dimensions. Detection under UV 366 nm. Also, HPLC and GC/MS. Discussion of the influence of the matrix and the applied methods on the results.

Anal. Biochem. 239, 115-117 (1996). TLC of sterols on silica gel with hexane - ethyl ether - acetic acid 80:15:1. Dried plates were rehydrated for 30 sec in PBS and then incubated for 30 min in filipin suspension at 37°C in the dark. Afterwards washed 2 x 2 min in water. Spots visualized at 365 nm. Detection limit 5 ng cholesterol.

J. Chromatogr. A 1026 (1-2), 301-304 (2004) A new and simplified method for extraction of ergosterol (ergosta-5,7,22-trien-3beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94-100 % depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaking extraction. Ergosterol was detected by TLC. Detection by fluorescence measurement, quantification limit was 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng.

Collaborative study. J. Chromatogr. 452, 399-408 (1988). Comparison of quantitative analysis of bile acids in biological samples with other methods. Discussion of the specificity, sensitivity, accuracy and simplicity of each. TLC gives reliable results for biliary bile acids except for differentiation between conjugates dihydroxycholanoic acids. The method compared were enzymatic analysis, radioimmunoassay, GC, HPLC and GC/MS.

J. Liq. Chrom. & Rel. Technol. 26, 2741-2750 (2003). HPTLC of bile acids (cholic, chenodeoxycholic, deoxycholic, lithocholic, glycocholic, glycodeoxycholic, and glycolithocholic acid) on RP-18 with mixtures of methanol - water. The methanol content was varied by 5% volumes from 60% to 100%. Detection after drying at room temperature by spraying with a 10% solution of sulfuric acid in water and heating at 120°C for 20 min. The retention parameters RMW may be used as a measure of lipophilicity of the investigated bile acids.

J. Chromatogr. 318, 373-377 (1985). TLC of various ecdysteroids on silica with chloroform - ethanol 4:1, on C18 -bonded silica with methanol - water 65:35 and on paraffin-coated silica plates with methanol - water 5:5. Detection by UV 254 nm. Rf values of 26 compounds are reported. Best results obtained with C18 bonded RP-HPTLC plates. (See also: I. Wilson et al., J. Chromatogr. 212, 211 (1981).)

J. Liquid Chromatogr. 18, 837-848 (1995). Description of the essential steps of displacement TLC and its applications for the separation of ecdysteroids. Discussion of the conditions of displacement TLC for plant ecdysteroids including the optimization of mobile phase composition, mobile phase flow rate, and preelution before displacement development.

Neighbouring group participation in hypobromous acid addition to 19-substituted 5 alpha-cholest-1-enes and in acid cleavage of their epoxy analogs. Coll. czechoslov. chem. comm. 47, 3062-3076 (1982). TLC of cholestane derivatives on silica with a) heptane-ether 4:1, b) petrol ether - ether - acetone 90:5:5, c) benzene - ether 1:1, 4:1 and 7:3. Quantification after elution.