J. Chromatogr. A 1530, 197-203 (2017). Evaluation of the phenolic and stigmasterol content and comparison of the antioxidant and antidiabetic activities by HPTLC combined with DPPH* free radical method and α-amylase assay towards ethanol and ethyl acetate extracts of 10 marine macroalgae species (3 chlorophyta, 4 phaeophyta and 3 rhodophyta). HPTLC on silica gel with n-hexane – ethyl acetate – acetic acid 20:9:1 over 80 mm. Detection either by dipping into 0.4 % DPPH* solution followed by storing in the dark for 30 min before evaluation, or by dipping into anisaldehyde – sulfuric acid reagent or neutralized ferric chloride solution (prepared by adding dilute sodium hydroxide to freshly prepared 2 % ferric chloride in methanol until precipitation occurs, followed by filtering), both followed by heating at 100 °C for 10 min. Quantitative evaluation by absorbance measurement at 470 nm for detection of α-amylase inhibition and at 550 nm for stigmasterol. The results showed higher antioxidant activity in the ethyl acetate extracts than in ethanol extracts, and higher amounts of fucoxanthin, stigmasterol and α-amylase inhibitory activities in ethyl acetate extracts. The results proved the relation of higher α-amylase inhibition in ethyl acetate extracts due to the presence of triterpenes and sterols, and no contribution of fucoxanthin to it.

Phytochemistry 25, 2779-2781 (1986). TLC of acetylated sterols on silver nitrate - silica gel 1:8 by developing the plates with freshly distilled chloroform. Detection with berberine. For best resolution of steryl acetates, 0.5 % carboxmethyl cellulose was mixed with the silica gel.

J. Chromatogr. 452, 543-547 (1988). TLC on silica with heptane - ethyl acetate 6:4 after oxidation with peracetic acid. Detection by spraying with 5% solution of phosphomolybdic acid in 2-propanol or 50% sulfuric acid . Quantification by densitometry. Also TLC/FID method.

Biochemical Systematics and Ecology 21, 467-473 (1993). TLC of cholesterol, campesterol, stigmasterol, ß-sitosterol, D5-avenasterol D7-stigmasterol and D7-avenasterol on silica with hexane - ethyl acetate 6:3. Steroids were silylated and the TMS-derivatives separated GLC.

J. Chinese Herb Med. (Zhongcaoyao) 30, 412-413 (1999). TLC on silica gel with petroleum ether (60-90°C) - ethyl ether 7:3. Detection by exposure to iodine vapor. Identification of b-amyrenonol, citrostadienol, b-sitosterol by comparison with the standards. Also GC and HPLC methods.

Planta med. 68, 980-985 (2002). Preparative TLC of pipersintenamide, piperarboricoline, sintenpyridone, ß-sitosterol, ß-sitostenone, and stigmasta-4,22-diene-3-one on silica gel with n-hexane - ethyl acetate 10:1, dichloromethane, dichloromethane - ethyl acetate 10:1, 20:1 and 30:1, chloroform, chloroform - methanol 30:1, and chloroform - acetone 15:1 and 20:1.

(TLC chromatographic analysis for the quantitative estimation of the fecal bile acid composition). TLC of cholic acid, chenodesoxy cholic acid, desoxy cholic acid, lithocholic acid and ursodesoxy cholic acid on silica with isooctane - isopropanol - acetic acid 30:10:1 and/or isooctane - ethyl acetate - acetic acid 10:10:2. Derivatization with 2 % ethanolic solution of 2,7-dichlorofluoresceine. Densitometric quantification by fluorescence. Fiber rich polysaccharides (dietary fibers) elevate fecal bile acids and their metabolites. The possible role of this diet in relation to fecal bile acid concentration and large bowel cancer is discussed.

J. Planar Chromatogr 7, 278-285 (1994). Study of the influence of the temperature, ionic strength, and apparent pH of the eluent to find an improved protocol for the analysis of conjugated bile acid. Comparison of the performance of different silica layers and variations of eluents at different temperatures. TLC at low temperature, the use of RP plates and the substitution of acetic acid with tetrahydrofuran leads to improvement of band shape, intensity and resolution.