J. Sep. Sci. 30, 2786-2793 (2007). HPTLC of unsaponifiable constituents of rice bran oil on silica gel in two stage separation: First separation with benzene – chloroform 12:1 for sterols, oryzanols, and tocols. Quantitative determination by absorbance measurement at 206 nm for sterols (1), 325 nm for oryzanols (2), and 297 nm for tocols (3). Second separation with petroleum ether – diethyl ether 50:1 for steryl esters (4), wax (5), and squalene (6). Detection by dipping in 5 % methanolic sulphuric acid followed by heating at 110 °C for 1 hour. Quantitative determination by absorbance measurement at 439 nm. The hRf values were 12 for (1), 21 for (2), 39 for (3), 36 for (4), 46 for (5), and 74 for (6). Linearity was between 150 and 1200 ng/zone for the first separation and between 400 and 1200 ng/zone the second separation. The limits of detection and quantification were 6 and 20 ng/zone for (1), 1 and 4 ng/zone for (2), 11 and 38 ng/zone for (3), 22 and 73 ng/zone for (4), 19 and 65 ng/zone for (5), and 3 and 10 ng/zone for (6), respectively. Intra-assay precision was between 0.52 and 1.94 % and inter-assay precision was between 0.87 and 2.27 %. Recoveries ranged from 93.5 to 101.9 %.

J. Planar Chromatogr. 30, 175-180 (2017). HPTLC of β-amyrin (1) and β-sitosterol (2) in the aerial parts of Tinospora cordifolia and Calotropis gigantia on silica gel with n-hexane – ethyl acetate 3:1. Detection by spraying with p-anisaldehyde reagent. Quantitative determination by absorbance measurement at 530 nm. The hRF values for (1) and (2) were 39 and 26, respectively. Linearity was between 100 and 1400 ng/zone for (1) and (2). LOD and LOQ were 18 and 55 ng/zone for (1) and 30 and 91 ng/zone for (2). The intermediate precision was <2 % (n=6). Recovery rate ranged from 98.4 to 99.3 % for (1) and 98.3 to 99.9 % for (2).

Lipids 17, 477-482 (1982). Preparative TLC of microbially produced metabolites of lithocholic acid and lithocholic acid-3 alpha-sulfate on silica with dichloromethane-methanol 95:5. Detection by spraying with anisaldehyde, followed by heating. The following metabolites were isolated: isolithocholic acid, 5 beta-cholanic acid, 3-keto-lithocholic acid, 5 alpha -cholestone and delta 3-cholenic acid.

J. Liquid Chrom. 10, 1277-1290 (1987). TLC on silica, Ag+ impregnated silica with e.g. isopropyl ether - acetic acid 96:4, then with petrol ether - acetic acid 90:10:1. Also chromatography on RP-18 silica. Derivatization with phosphomolybdic acid reagent, for quantification with copper acetate - H3PO4 reagent. Quantification by densitometry.

Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 49-55 (1991). Separation of ceramides and cholesterol on silica, using a four-step 2-D developing technique: first direction with chloroform - acetone - methanol 90:5:5 over 1 cm, second direction with the same solvent over 8.5 cm, then in the first direction again with chloroform - acetone - methanol - NH3 60:30:8:2, and finally with chloroform - acetone - methanol - acetic acid - water 78:16:4:2:1 in the second direction. Derivatization by dipping in phosphoric acid - acetic acid - sulfuric acid - water 10:10:1:180 followed by heating at 200 °C for 10 min. Method for routine analysis of ceramides.

Chinese J. Herb Med. (Zhongcaoyao) 24, 128-130 (1993). TLC of palmityl-ß-sitosterol, lupenone, 24-methylene-cycloartenol, and ß-sitosterol on silica with chloroform - benzene 9:1. Detection by spraying with 5% phosphomolybdic acid. Quantification by densitometry (absorbance) at 625 nm.

J. Planar Chromatogr. 13, 437-442 (2000). TLC of cholesterol and oxysterols (7-ketosterol, 25-hydroxycholesterol, 7b-hydroxycholesterol, cholesterol-5a,6a-epoxide, cholestane-3b,5a,6b-triol) on silica gel with chloroform-acetone 9:1. After spraying with Liebermann-Burchard reagent the spots were quantified by densitometry in reflectance mode at 360 nm. Also TLC of silylated oxysterols on silanized silica gel with n-hexane - ether - methanol 38:2:1. Simple, sensitive, and reproducible method.

J. Sep. Sci. 33, 3110-3118 (2010). HPTLC of bile acids and their derivatives on 1) RP-18, 2) RP-18W and 3) cyano phase with methanol - water mixtures. The volume fraction of organic solvent in the mobile phase ranged from 70-90 % for (1), 50-70 % for (2) and 45-65 % for (3). Detection by spraying with a solution of manganese chloride in sulfuric acid, followed by heating at 100-120 °C for 10 min. Quantification by absorbance measurement at 254 nm and 365 nm.