Pharmagnostic identification and determination of organic constituents.) (Chinese). J. Chinese Herb Med. (Zhongcaoyao) 19, 554-557 (1988). TLC of oleanolic acid on silica with toluene - ethyl acetate - acetic acid 24:8:1. Detection by spraying with 5% phosphomolybdate reagent and heating at 110°C for 10 min. Quantification by densitometry at 625 nm. TLC of ecdysterone on silica with ethyl acetate - ethanol 4:1. Detection by heating at 125°C for 3 min. and spraying with 20% sulfanilic acid reagent and vanillin - acetic acid - sulfuric acid 0. 5:50:1. Quantification by densitometry, at 435 nm.

J. Planar Chromatogr. 4, 267-269 (1991). HPTLC of testosterone on silica with benzene – hexane – ethanol. Detection by spraying with 0.005 m 1,4-dihydrazinophthalazine in sulfuric acid – ethanol 1:1. Quantification by densitometry (absorbance at 400 nm). Detection limit 0.125 µg% plasma testosterone and 1 µg/1000 mL 24 h urinary testosterone. Simple, sensitive, selective, rapid TLC procedure.

J. Planar Chromatogr. 6, 341-345 (1993). Use of color slide films for the selection of appropriate filters and exposure data. Color prints are then made with color negative film using the previously optimized condition. With this method the number of photographs can be limited to one of each chromatogram. TLC of steroids (hydrocortisone, prednisolone, mesylate, hydrocortisone acetate, and spironolactone, trenbolone acetate, hydroxyprogesterone caproate, pregnadienolone acetate oxime ) with benzene - ethyl acetate 1:1. Visualization by spraying with a 10% ethanolic solution of sulfuric acid, followed by heating at 100 °C for 2 to 4 min.

J. Agric. Food Chem. 48, 231-234 (2000). TLC of the unsaponifiables (hydrocarbons, triterpene alcohols, sterols) on silica gel, bandwise applied, with hexane - ethyl acetate 6:1. Detection after spraying with 0.5% Rhodamine 6G solution and observation under UV. After elution and extraction silanation of sterols and triterpene alcohols with BSTFA for GC investigation.

J. Chromatogr. 329, 171-177 (1985). Two-dimensional TLC of unconjugated metabolites of neutral steroids on silica with 1) 1,2-dichloroethane - methyl acetate 8:2 and 2) hexane -1-hexanol 65:35. Detection with Liebermann-Burchard reagent.

J. Planar Chromatogr. 2, 33-38 (1989). Two-dimensional TLC of anabolics on silica with 5 different solvent systems. Reference mixtures contained 2 - 20 ng of the steroids (zeranol, zearalenone, a-nortestosterone, medroxyprogesterone acetate, trenbolone, trebolone acetate). Alternatively a „4x4“ developing mode could be used. In this mode 4 samples are developed in two dimensions on one HPTLC plate. For inducing fluorescence reaction, the plates were dipped into a 5% sulfuric acid - ethanol solution for 30 s and then incubated at 95°C for 10 min. The fluorescence before and after heating was observed under UV 366 nm. The described method permit the routine detection of various anabolic residues in bovine urine at levels of 0.5 - 10 ppb. The recoveries are high (70 - 90%). Standard deviation were between 2 and 8%.

CBS 93, 10-12 (2004). HPTLC of progesterone on silica gel prewashed by development in AMD2 first with chloroform - methanol 1:1 and then with the mobile phase, followed by drying at 80 °C for 15 min. Development in AMD2 with toluene - 2-propanol 10:1 without preconditioning over 60 mm. Quantitative determination by absorbance measurement at 252 nm followed by spectra recording from 200 to 360 nm. The linear working range is 25.7-154.5 ng/zone. Repeatability (standard deviation calculated from the amounts of seven simulated progesterone samples determined on the same plate at three concentration levels in the lower, middle and upper range) is 0.26-1.29 %. Recovery is 99.88-100.97 %. Reproducibility was performed with recycled HPTLC plates.

J. Planar Chromatogr. 30, 423-426 (2017). 2D-HPTLC of phytoestrogenic active compounds in the root of Glycyrrhiza glabra on RP-18 with hexane – ethyl acetate – acetone 9:3:2 in the first direction and acetone – water 3:2 in the second direction. Effect-direct analysis by dipping into a yeast suspension followed by incubation at 30 °C for 4 h, drying at 37 °C for 15 min and spraying with the combined reaction buffer C (20 mL reaction buffer C is mixed with 0.2 mL of a freshly prepared solution of 0.05 g/mL 4-methylumbelliferyl-ß-D-galactopyranoside in DMSO or 0.2 mL of a freshly prepared solution of 0.05 g/mL 5-bromo-4-chloro-3-indoxyl-ß-D-galactopyranoside (X-Gal) in DMSO). Fluorescence detection at UV 366 nm._x000D_