J. Planar Chromatogr. 26, 375-378 (2013). TLC of estrone, estradiol, and estriol on silica gel with chloroform - ethyl acetate - acetone 6:2:1. Detecion by dipping into (1) 10 % solution of phosphomolybdic acid (PMA) in methanol, followed by heating at 100 ºC for 10 min; (2) 0.2 % ceric ammonium sulfate in phosphoric acid, followed by heating at 110 ºC for 10 min; (3) 0.2 g manganese(II) chloride in 30 mL water, 30 mL methanol and 2 mL concentrated sulfuric acid, followed by heating at 100-120 ºC for 10-15 min; and (4) 1 g of vanillin in 25 mL of ethanol, 25 mL of distilled water, and 35 mL of ortho-phosphoric acid (85 %), followed by heating at 120-160 ºC for 5-15 min. The lowest detection limit for estrone (75 ng/zone) was achieved using (1) and (3), whereas for estradiol and estriol (both 4.7 ng per zone) by using (2).

J. Planar Chromatogr. 2, 33-38 (1989). Two-dimensional TLC of anabolics on silica with 5 different solvent systems. Reference mixtures contained 2 - 20 ng of the steroids (zeranol, zearalenone, a-nortestosterone, medroxyprogesterone acetate, trenbolone, trebolone acetate). Alternatively a „4x4“ developing mode could be used. In this mode 4 samples are developed in two dimensions on one HPTLC plate. For inducing fluorescence reaction, the plates were dipped into a 5% sulfuric acid - ethanol solution for 30 s and then incubated at 95°C for 10 min. The fluorescence before and after heating was observed under UV 366 nm. The described method permit the routine detection of various anabolic residues in bovine urine at levels of 0.5 - 10 ppb. The recoveries are high (70 - 90%). Standard deviation were between 2 and 8%.

J. Liq. Chromatogr. Relat. Technol. 30, 2329-2335 (2007). HPTLC of sterols and fatty acids on silica gel (prewashed with methanol) with petroleum ether (36-60 °C) - diethyl ether - acetic acid 80:20:1 in a twin-trough chamber with chamber saturation. Detection by spraying with a 5 % ethanolic phosphomolybdic acid solution followed by heating at 110 °C for 10 min. Quantitative determination by absorbance measurement in the visible range.

J. Planar Chromatogr. 30, 495-501 (2017). HPTLC of stigmasterol in Lasiurus scindicus on silica gel with toluene ‒ methanol ‒ formic acid 90:10:3. Detection by spraying with anisaldehyde ‒ sulfuric acid reagent, followed by heating at 150 °C for 5 min. Quantitative determination by absorbance measurement at 530 nm. The hRF value for stigmasterol was 27. Linearity was between 2 and 12 ng/zone. LOD and LOQ were 0.07 and 0.2 ng/zone. Average recovery was 99.3 %.

V. Preparation and investigation of steroid-17-spiro-1',3',2'-oxazophospholidines and -1',3',2'-dioxaphospholanes. Acta Chimica 116, 125-129 (1984). TLC of the title compounds on alumina with chloroform - ethyl acetate 3:1. Detection with sulfuric acid and heating at 110 °C.

J. Microchem. 37, 174-180 (1988). TLC of cholesterol, cholesteryl formate, cholesteryl acetate, cholesteryl propianate on silica with 5 solvent systems (e.g. hexane - chloroform - ether - methanol - acetic acid 2:15: 60:5:2 or hexane - chloroform - ether - methanol - acetic acid - 1-propanol 920:15:6:2:2:3. Detection by spraying with 30 g copper acetate in 80 mL H3PO4 made up to 1 L and heating at 130°C for 5 min.

J. Chromatogr. 532, 449-452 (1990). TLC on silica with petrol ether - ethyl acetate - acetic acid 80:20:1. Detection by spraying with vanillin - sulfuric acid 1:100 (w/v) and heating at 85 °C for 6 min. Quantification by densitometry at 525 nm using external standards.

J. Liquid Chromatogr. 18, 527-535 (1995). HPTLC of cholesterol esters, triacylglycerols, free fatty acids, and cholesterol on silica with petrol ether (37.5 - 52 °) - ether - acetic acid 80:20:2, and hexane - petrol ether - ethyl ether - acetic acid 50:20:2:1. Detection by spraying with 5% phosphomolybdic acid in ethanol and heating at 110-120 °C for 5-10 min. Quantification by densitometry at 700 nm.