J. Planar Chromatogr. 31, 72-78 (2018). HPTLC of equol in ethanolic cattle manure extract on RP-18 with n-hexane – ethyl – acetate – acetone 9:3:2. Detection by planar yeast estrogen screening (pYES) by dipping into a yeast suspension, followed by incubation at 30 °C for 4 h. After incubation, the plate was dried in a 37 °C incubator for 15 min and dipped into the combined reaction buffer followed by incubation at 37 °C for 60 min and 90 % relative humidity. The combined reaction buffer was prepared by mixing 20 mL of buffer C (5.3 g of sodium phosphate dibasic and 0.4 g of potassium chloride were dissolved in about 490 mL water, the solution was adjusted with sodium hydroxide to pH 13, 0.5 g of benzalkonium chloride were added and the mixture was filled up to 500 mL) and 0.2 mL of a freshly prepared X-Gal solution (0.05 g/mL X-Gal in DMSO). Fluorescence evaluation under UV 366 nm. The hRF value for equol was 47.

J. Chromatogr. Sci. 34, 330-333 (1996). Two-dimensional TLC on silica with 1) chloroform - acetone 9:1, 2) cyclohexane - ethyl acetate - ethanol 31:8:1 for both directions. Detection by spraying with 95% ethanol concentrated sulfuric acid, heating at 110°C and visualization under UV. Quantification by HPLC.

J. Planar Chromatogr. 28, 152-156 (2015). HPTLC of cholesterol from lung tissues on silica gel with petroleum ether - diethyl ether - acetic acid 90:10:1 in an unsaturated chamber. Detection by dipping in phosphomolybdic acid. Quantitative determination by absorbance measurement at 580 nm. Comparable results were obtained by HPLC analyses of the same samples.

J. Sep. Sci. 41, 4304-4314 (2018). Review of TLC methods for the analysis of ecdysteroids with particular emphasis on extracts made of aerial parts of Maral root. TLC fingerprinting and TLC-coupled methods of detection of ecdysteroids were also described, including 2D-TLC procedures in NP and RP modes.

Biochemical Systematics and Ecology 13,. 167-168 (1985). TLC on silica with benzene -ether 1:1. Separation of an aliquot of the crude sterol fraction from H. disticha and elution of the bands Rf.=0.65 (cholesta-5,23-diene-3 beta, 25-diol) Rf=0.55 (cholesta-5,25-diene-3 beta, 24-diol).

Phytochemistry 28, 465-468 (1989). TLC of 4,4-dimethyl, 4+-methyl and 4-dimethyl sterols on silica with chloroform - methanol - acetic acid 272:3:3 and butanol - benzene 1:9. Visualization under UV and with 0.05% berberine in 95% ethanol.

J. Liquid Chromatogr. 15, 2967-2980 (1992). TLC of triacylglycerols on silica and AgNO3-impregnated silica and silica with isopropanol - chloroform 3:197. TLC on RP-18 silica with acetonitrile - MEK - chloroform 10:7:3. Detection by spraying with 5% phosphomolybdic acid in ethanol for plain silica and RP-18 silica, and with 50% sulfuric acid for silver-loaded silica. Comparison of systems and detection.

Biochemical Systematics and Ecology 26, 365-366 (1998). TLC of sterols on silica gel with chloroform -methanol 2:1 or chloroform - butyl acetate 3:1.